Bio-printed 3D liver model and high-content imaging for assessment of compound toxicity effects

Bio-printed 3D liver model

Improve the reproducibility of non-clinical drug safety predictions using three-dimensional (3D) cellular models.

3D bioprinting enables the generation of complex models with spatial control and a variety of matrices allowing the formation of complex tissue structures. Here we describe a method for automated generation of 3D cellular liver models using a multi-tool robotic device with liquid handling, enabling the automated bioprinting and high-content imaging of liver cells within a collagen matrix. This assay was used for compound testing and evaluation of toxicity effects in liver cells. As an example, in an assay using bio-printed structures, we used HepG2 cells resuspended in collagen I with micro-structure printed channels allowing better diffusion of media. Using a printing tool and pipettor, the robot printed Pluronic acid into wells of a multi-well plate to create the 3D structures needed for channel formation then dispensed the cell mix with collagen to create a thick 3D cell layer.

Learn more about the systems behind the methods:

ImageXpress Micro Confocal system

High-content confocal imaging solution with proprietary spinning disk technology and water objective options

ImageXpress Micro 4
  • Ideal for 3D organoid and spheroid imaging
  • Proprietary AgileOptix™ spinning disk technology
  • QuickID reduces image acquisition time and data storage requirements
  • Water immersion objectives enhance resolution, sensitivity and throughput of complex
    3D assays
  • Lasers and deep tissue confocal disk provide deeper penetration into 3D samples
  • 3D volumetric analysis

Learn more

BioAssemblyBot 400

Intelligent, precise, and scalable automation solution for 3D biology

Produce and maintain living tissues, organoids, and spheroids more consistently than ever before. Then analyze them without ever touching the well plate. BAB400 integrates seamlessly with the ImageXpress Confocal to round out your integrated organoid workflow with automated passaging, cell culture monitoring, suspension dispensing, and 3D bioprinting of tissues and organoids.

Learn more

BAB400 Spine

IN Carta Image Analysis Software

Modern machine learning, high-content image analysis software

In Carta

Powerful analytics combined with an intuitive user interface simplify workflows for advanced phenotypic classification and/or 3D image analysis. Advanced features provide the functionality you need to analyze data at scale and deliver real-time insights without the need for complex pre- or post- processing operations. Put machine learning to work for you and perform complex classification automatically with the optional Phenoglyphs module.

Learn more

MetaXpress software for ImageXpress high-content imaging systems

Multi-level analysis tools for a wide range of applications

  • Meet high throughput requirements with a scalable, streamlined workflow
  • Adapt your analysis tools to tackle your toughest problems, including 3D analysis
  • Schedule automatic data transfer between third-party hardware sources and secure database
  • Set up hundreds of routinely used HCS assays using MetaXpress software modules
  • Export data to IN Carta software, leveraging intuitive, modern machine learning

Learn more


More Great Resources