Optimizing animal-free CloneDetect assay to maximize the sensitivity of real-time local detection of human IgG antibody production using ClonePix system

How to screen and select clones of interest with high specificity and high efficiency is known as one of the bottlenecks for therapeutic antibody engineering and cell line development.

In this study, we have explored the usage of recombinant Pro A or Pro G, the two most commonly used proteins for the purification of IgG antibodies in the Biopharma manufacturing process, combined with CloneDetect K8495 to enhance the fluorescent signal for IgG detection/quantification.