Complex event analysis for neurotoxic profiling of compound effects on human iPSC-derived neural spheroid 3D cultures
We describe a method for the complex analysis of calcium oscillations using the FLIPR® Penta High-Throughput Cellular Screening System that allows detection and multi-parametric characterization of oscillation peaks. ScreenWorks® Peak Pro 2 analysis software analyzes calcium oscillation rate, peak width and amplitude, characterization of secondary peaks, waveform irregularities, and several other read-outs.
High-throughput assessment of compound-induced pro-arrhythmic effects in human iPSC-derived cardiomyocytes
Development of biologically relevant and predictive cell-based assays for compound screening and toxicity assessment is a major challenge in drug discovery. The focus of this study was to establish high-throughput, compatible cardiotoxicity assays using human induced pluripotent stem cell (iPSC)-derived cardiomyocytes.