Capture True 3D Cell Culture Insights

What you will find in this eBook

Development of more complex, biologically relevant, and predictive cell-based assays for compound screening is a primary challenge in drug discovery. The integration of three-dimensional (3D) assay models is becoming more widespread to drive translational biology. Specifically, 3D cultures offer the advantage of closely recapitulating aspects of human tissues including the architecture, cell organization, cell-cell and cell-matrix interactions, and more physiologically-relevant diffusion characteristics.

This ebook highlights the 3D cell culture imaging and analysis advantage and includes a few 3D cell model applications as well as tips and techniques for optimizing your 3D assay workflow.

Applications
Physiologically-relevant tissue models using high-throughput organ-on-a-chip platform
Functional evaluation of neurotoxic and neuroactive compound effects in iPSC-derived 3D neural co-cultures
High-content assay for morphological characterization of neuronal development in 3D matrix
Assessment of drug effects on cardiomyocyte physiology using human iPSC-derived cardiac spheroids
Using spheroid models to develop relevant highthroughput assays with quantitative endpoints

Tips and Techniques
Automated 3D cell culture and screening by imaging and flow cytometry
Automation of 3D spheroid cultures in ultra-low attachment plates
Multi-parameter imaging assay for measuring toxicity in a tumor model
Measuring mitochondrial shape and distribution
Increase sensitivity in no-wash assays using confocal imaging

Sample pages

3D Imaging Advantage

Organ-on-a-chip Platform

Neural Co-culture Platform

3D Hydrogel

Automated 3D Cell Culture and Screening

SIMPLIFY 3D CELL
CULTURE IMAGING AND
ANALYSIS

Capture True 3D Cell Culture Insights

Whatever your imaging requirement, we have a solution
that will meet the needs of your lab and your budget.

3D Cellular Imaging Systems

Our ImageXpress portfolio of cellular imaging systems and analysis software offers innovative capabilities for evaluating complex and emerging 3D cell culture-based models such as:

Organoids
Spheroids
Whole organisms
Induced Pluripotent Stem Cell (iPSC)-derived cell models
Organ-on-a-chip technologies

	ImageXpress Pico Automated Cell Imaging System	ImageXpress Nano Automated Imaging System	ImageXpress Micro 4 High-Content Imaging System	ImageXpress Micro Confocal High-Content Imaging System
CAMERA	5 megaPixel CMOS	4.7 megaPixel CMOS	>4 megaPixel CMOS	>4 megaPixel CMOS
FIELD OF VIEW 10X	1.93 mm²	1.85 mm²	1.96 mm²	1.96 mm²
OBJECTIVES	4x-63x Air	2x-60x Air	Air (1x-100x), Oil (40x, 60x, 100x)	Air (1x-100x), Oil (40x, 60x, 100x), and Water (20x, 40x, 60x)
FILTERS CHANGER	5 position filter cube changer	5 position filter cube changer	5 position filter cube changer	8 position emission wheel and 5 position dichroic filter wheel
AUTOFOCUS	LED+ image autofocus	Laser+image autofocus	Laser+image autofocus	Laser+image autofocus
PLATES	Up to 384-well plates	Up to 1536-well plates	Up to 1536-well plates	Up to 1536-well plates
TEMPERATURE CONTROL OPTION