Understanding organoid morphology using 3D image analysis

Organoid Morphology using 3D Image Analysis

This study assesses patient-derived organoid size and cell count from 3D image analysis.

Patient-derived Organoids are the drug discovery platform of the future because of their greater physiological and clinical relevance as compared to existing 2D models used in screening. Importantly, this work demonstrates the principle that morphological parameters can be assessed and quantified in a high-throughput format.

Once key morphometric signatures for clinical efficacy have been defined, 3D imaging has the potential to revolutionize drug discovery, leading to the early selection of hit compounds that will progress through to the clinic. Assays for changes in organoid morphology following drug-treatment have the potential to identify treatments targeted to specific cell-types such as cancer stem cells, that would be missed in the metabolic assays used currently, since there is no bulk cell-killing effect.

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ImageXpress Micro Confocal system

High-content confocal imaging solution with proprietary spinning disk technology and water objective options

ImageXpress Micro 4
  • Ideal for 3D organoid and spheroid imaging
  • Proprietary AgileOptix™ spinning disk technology
  • QuickID reduces image acquisition time and data storage requirements
  • Water immersion objectives enhance resolution, sensitivity and throughput of complex
    3D assays
  • Lasers and deep tissue confocal disk provide deeper penetration into 3D samples
  • 3D volumetric analysis

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MetaXpress software for ImageXpress high-content imaging systems

Multi-level analysis tools for a wide range of applications

Introducing the MetaXpress 3D Analysis Toolkit
  • Meet high throughput requirements with a scalable, streamlined workflow
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