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High-throughput screening of 3D cell cultures with multiple high density scaffold-free spheroids for cancer toxicity studies

Here, we demonstrate the use of the Corning® Elplasia® 96-well plates with a 3D culture workflow that includes spheroid generation, compound treatment, cytotoxicity assay, 3D imaging on the ImageXpress® Micro Confocal High-Content Imaging System, and 3D image analysis using MetaXpress® High-Content Image Acquisition and Analysis Software.

The ability to easily generate multiple spheroids and to seamlessly integrate the workflow with high-content imaging will have significant applications in drug discovery and compound toxicology

  • Easily increase the number of spheroids per experimental condition
  • Grow, stain, and image large numbers of spheroids simultaneously
  • Use high-content imaging for simultaneous 3D analysis of multiple spheroids or organoids

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