APPLICATION NOTE
Reliable Thrombin Generation on the SpectraMax® i3x Multi-Mode Microplate Reader
Perform standardized thrombin generation assays using Technothrombin® TGA reagents on the SpectraMax® i3x microplate reader with validated fluorescence detection and data analysis workflows.
Standardized and reproducible thrombin generation measurement
Standardized thrombin generation measurement
Use Technothrombin® TGA reagents with defined trigger conditions to generate reproducible thrombin generation curves across plasma samples.
Validated performance on the SpectraMax® i3x reader
Achieve precise fluorescence‑based kinetic measurements with demonstrated intra‑ and inter‑assay CVs below 10% using validated reader settings.
Time‑resolved coagulation analysis
Calculate key thrombin generation parameters—including lag time, peak thrombin, velocity index, and area under the curve—to characterize the full coagulation process.
Flexible assay configuration for multiple research applications
Apply different TGA trigger reagents to study thrombophilia, hemophilia, anticoagulation therapy, and thrombogenic activity using a single platform.
Integrated data acquisition and analysis workflow
Combine SoftMax® Pro Software with the Technothrombin TGA evaluation file to streamline data analysis and reuse a single calibration curve per substrate lot.
