APPLICATION NOTE
Run and analyze fast kinetic enzyme assays
Capture fast reactions before early timepoints are lost. See how automated injection, rapid reads, and SoftMax Pro analysis streamline enzyme kinetics from setup to results.
Keep up with reactions that will not wait
Run and analyze a fast kinetic enzyme assay with injectors on SpectraMax iD Series Multi-Mode Microplate Readers
When an enzyme reaction happens in seconds, manual pipetting and plate transfers can introduce variability and cost you early timepoints. Download this step-by-step application note to see how SpectraMax® iD Series readers with injectors and SoftMax® Pro software streamline a fast kinetic protease assay from setup to analysis.
Discover how to:
- Standardize reagent addition with SmartInject™ technology, which combines simultaneous injection and plate shaking for reagent mixing.
- Follow fast reactions with kinetic measurements every 2 seconds in the demonstrated assay.
- Automate initial velocity calculations, curve fitting, and data analysis in SoftMax Pro software.
- Reduce reagent waste with an injector system dead volume of less than 10 µL.
- Save acquisition and analysis settings as a reusable protocol for future experiments.
The assay was demonstrated on the SpectraMax iD5 reader; the workflow is also compatible with iD3, iD3s, and iD5e readers equipped with injectors.
Thank you for your interest.
We’re excited to share with you our latest learnings – from new product innovations to improved methods for automating complex biology workflows – our experts look forward to connecting with you and exploring ways to advance scientific research, together.