APPLICATION NOTE
Sensitive quantitation of plasmid DNA from bacteria cultured in microplates
Sensitive, high-throughput quantitation of plasmid DNA from microplate-cultured bacteria using PicoGreen fluorescence and SpectraMax® Multi-Mode Microplate Readers.
Accelerate your molecular biology workflows with a robust, sensitive, and scalable method for quantifying plasmid DNA directly from bacteria grown in microplates. This application note demonstrates how to streamline DNA prep and analysis using ready-to-use protocols, enabling reliable results for cloning, sequencing, and synthetic biology—all with minimal sample volume and hands-on time.
Key Features:
- Sensitive fluorescent quantitation of DNA down to 50 pg/mL
- Linear dynamic range spanning over four orders of magnitude
- Compatible with 96-well microplate format for high-throughput workflows
- Ready-to-use protocols in SoftMax® Pro Software for streamlined analysis
- Supports both SpectraMax® iD3s and iD5e Multi-Mode Microplate Readers
Benefits:
- Enables accurate plasmid DNA quantitation from small-volume bacterial cultures
- Reduces hands-on time and increases throughput for molecular biology labs
- Facilitates reliable downstream applications such as cloning, PCR, sequencing, and transfection
- Provides reproducible results with automated data analysis and reporting
- Adaptable to a wide range of research and assay development needs
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Product family
Microplate Reader
Product primary application
SpectraMax iD3s and iD5e Multi-Mode Microplate Readers; Nucleic Acid Detection Quantitation Analysis; Microbiology and Contaminant Monitoring; Drug Discovery and Development; Toxicology; Vaccine Development Workflows; Plasmid Production; Quality Control and Safety Testing; Absorbance; Fluorescence
CMP
701Nr00000msC2BIAU
Title
Sensitive quantitation of plasmid DNA from bacteria cultured in microplates
Resource URL
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