Quantify TNFα secretion by THP-1-derived macrophages with an AlphaLISA assay

A major cellular component of the tumor microenvironment, tumor-associated macrophages are often associated with poor prognosis and are under investigation as potential targets for anticancer therapy.

Macrophages are a type of white blood cell that plays a role in detecting and destroying infectious microorganisms, removing dead cells and debris, and in wound healing. 

In this application note, effects of several anti-inflammatory compounds on TNFα secretion by THP-1-derived macrophages were measured using the AlphaLISA High Performance (HP) Human Tumor Necrosis Factor alpha (TNFα) Detection Kit. Using the SpectraMax i3x Multi-Mode Microplate Reader and this homogeneous assay, picogram levels of TNFα were detected in culture medium removed from THP-1 cells activated with PMA and LPS, and further treated with the compounds SB202190, stattic, and resveratrol.