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Application Note

Optimized settings for Transcreener TR-FRET assays on SpectraMax iD5 and i3x readers

  • Single-addition, mix-and-read assay format for high-throughput screening
  • Minimal interference from fluorescent compounds with red tracer and time-resolved readout
  • Z’ factor > 0.7 at 10% conversion of 10 μM ATP