
APPLICATION NOTE
Improved cell viability for multi-day cell-based microplate reader assays
Maintain optimal cell health and assay reliability over extended times with SpectraMax® iD3s/iD5e readers and the SpectraMax® aer Gas Mixer—ideal for long-term live-cell workflows.

Enhanced Cell Viability for Multi-Day Assays Using SpectraMax® Readers with Gas Control
- Improved Cell Viability Over Time
Achieve 3-day viability comparable to standard incubators using controlled CO₂ and O₂ environments. - Integrated Gas Control
SpectraMax® aer Gas Mixer enables precise environmental tuning (0.1–15% CO₂, 1–21% O₂) directly within the reader chamber. - Streamlined Workflow
SoftMax® Pro Software automates kinetic data collection with preconfigured protocols—no manual intervention required. - Flexible Assay Compatibility
Supports both RealTime-Glo™ and CellTiter-Glo® assays for continuous or endpoint viability measurements. - Consistent, Reproducible Results
Reduced variability and evaporation with sealed plates and edge-well buffering ensure reliable long-term data. - Ideal for Drug Screening & Toxicology
Enables extended monitoring of cell response to treatments in pharmaceutical, environmental, and toxicology research.
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Product family
Microplate Reader
Product primary application
SpectraMax iD3 and iD5 Multi-Mode Microplate Readers
CMP
701Rn00000gMJTeIAO
Title
Improved cell viability for multi-day cell-based microplate reader assays
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