Application Note

Cell-based measurement of ERK1/2 phosphorylation with THUNDER TR-FRET assay

  • Highly robust homogeneous (no-wash) assay with simple and fast assay workflow
  • Highly sensitive assay generating well-defined, complete concentration-response curves without the need to serum starve the cells
  • EC50 and IC50 values in agreement with published data
  • Suitable for both academic and drug discovery applications