Octet Platform Solution
The group was able to develop a working Fab activity assay on the Octet RED system in less than a week. Relative to the overnight incubation and four-hour assay time of their ELISA protocol, the Octet assay provided an analysis time of only one hour per 96-well microplate, including sample preparation time. This Octet assay was used to monitor Fab activity for all process development studies. The assay was subsequently qualified for use in quality control in less than one month. This document describes the various steps involved in development and initial validation of the Octet assay and presents experimental results from Boehringer Ingelheim that demonstrate the value of Octet systems in process development and QC studies.
Assay Development And Results
An Octet RED instrument and Streptavidin biosensors loaded with a biotinylated small molecule ligand (capture molecule) were used for all experiments. The Fab samples were prepared in a PBS formulation buffer containing BSA and were analyzed in 96-well, black, polypropylene, flat-bottom microplates from Greiner (Part No. 655209). The assay format ensured detection of active Fab molecules only; degraded, inactive or clipped variants of the molecule do not bind to the anti-Fab ligand on the biosensor. Results from the quantitation assay are expressed in terms of percent activity of the Fab molecule, calculated as the ratio of the Fab concentration determined by the Octet assay to the concentration value determined by A280 absorption spectroscopy.
Low Ligand Consumption
The small molecule ligand for the Fab was synthesized with a 1:1 biotin:ligand molar ratio. Loading onto Streptavidin biosensors was optimized for ligand concentration and loading time by incubating a range of concentrations for 800 seconds at 30°C with 1000 RPM shaking of the microplate (Figure 1). Real-time monitoring showed that 1.25 μg/mL of ligand incubated for 60 seconds at 1000 RPM resulted in optimal loading on the biosensor. These parameters were chosen as final conditions for all subsequent studies.
Biosensors Regenerated and Re-used
Many Pall ForteBio biosensors can be regenerated and re-used for multiple tests. Effective re-use of a biosensor requires careful optimization of the regeneration protocol. For the Fab assay, phosphoric acid, citric acid, glycine and sodium hydroxide were screened as regeneration solutions. Sodium hydroxide treatment achieved partial regeneration. Complete regeneration for 10 cycles was seen when 50 mM sodium hydroxide buffer was supplemented with 1% sodium dodecyl sulfate (SDS) (Figure 2).
Linear Dynamic Range Greater than 2 Logs
Purified bulk drug substance was diluted in PBS buffer containing 0.1% BSA. To determine the dynamic range for detection of the Fab molecule, concentrations from 15.6 μg/mL to 2 mg/mL were tested in quadruplicate (Figure 3) using a microplate shake speed of 400 RPM and a 2-minute read time per sample. The two highest concentrations, 1 and 2 mg/mL, saturated the biosensor quickly, resulting in high CVs and unacceptable deviation from linearity. A lower range of Fab concentrations (3.13-400 μg/mL) was then tested in quadruplicate. This range provided acceptable results, with each concentration exhibiting a low CV and a calculated recovery within 10% of the theoretical values (Figure 3). The entire operating range of the standard curve was linear, with an R2= 0.999 (Figure 4). As the linearity, accuracy and precision at each concentration in the dynamic range were optimal, 3.13-400 μg/mL was selected to be the operating range for the Fab activity assay.
|Theoretical (µg/mL)||Octet assay (µg/mL)||%CV||%Accuracy|
|Theoretical (µg/mL)||Octet assay (µg/mL)||%CV||%Accuracy|
Saved Standard Curves are Reliable
In Octet Data Analysis software, standard curves can be saved and applied to future experiments. To validate this approach, a standard curve was generated on Day 0 that was used to quantify the control sample prepared on Day 0 and on two subsequent days (Days 1 and 2). As shown in Figure 5, calculated percent activity values of the control sample for all three days were statistically identical. Control sample at four dilutions, in duplicate, were run with unknowns in all subsequent experiments to monitor the robustness of the assay.
|Control lot||Day||A280 (µg/mL)||Octet assay (µg/mL)||% Activity|
Octet Fab Activity Data Reliable Regardless of the Type of Curve-Fitting
Octet Data Analysis software provides several data-fitting options including linear point-to-point, 4P (weighted or unweighted), and 5P (weighted or unweighted). Four Fab samples of known concentration and four other control samples were each assayed at four dilutions in duplicate, and the results were analyzed using linear point-to-point and four-parameter data fits (Figure 6). The known samples recovered within ±5% of the expected values and the eight replicates were measured with low CVs (<5%), regardless of the curve fitting model.
Specific Detection of Fab Fragments in the Presence of Monoclonal Antibodies
Six different monoclonal antibodies were titrated and tested with the Octet assay. None of the six antibodies produced a binding signal, demonstrating the high specificity of the Octet assay for the Fab molecule (Figure 7).
During Fab production, the culture medium contains many host cell proteins in addition to the Fab. The assay must display high specificity for the intact Fab molecule in the presence of these impurities during the purification process. To test for assay specificity and accuracy, Fab samples were enriched for one of the major host cell contaminants. The purity of the samples was verified by size exclusion chromatography and capillary electrophoresis, and determined to be of 50% and 5% Fab content. The enriched impurity samples were run in the assay and the percent activity directly correlated with percent purity of the sample, i.e., 50% purity yielded 49% activity and 5% purity yielded 4.2% activity (Figure 8). The Octet assay demonstrated excellent accuracy for the Fab molecule over the entire range of impurity levels.
|Sample||Octet assay (µg/mL)||A280 (µg/mL)||%CV||% Activity||% Accuracy|
Octet Assay Complements A280 Spectroscopy for Inprocess Testing
A280 spectroscopy cannot differentiate between the Fab molecule and in-process impurities, instead measuring total protein concentration in the sample. The Octet assay specifically detected the Fab molecule in the presence of impurities. The Fab concentration values reported by the two methods showed that the first purification step resulted in a partially purified sample containing approximately 15% of a major host cell impurity (Figure 9), and was confirmed by a secondary method.
Octet values correlated very well (within 5%) with A280 spectroscopy values for the purified Fab molecule following the first purification step (Figure 9).
|Sample||Octet assay (µg/mL)||A280 (µg/mL)||%CV||% Activity||% Major impurity|
|First purification step||7680||9500||2.6||81||15%|
|First intermediate step||4220||4380||4.4||96||<1%|
|Second intermediate step||7570||7680||1.8||99||<1%|
|Final purification step||67880||65790||7.6||103||<1%|
|Bulk drug substance||50100||49500||5.0||101||<1%|
Octet Fab Assay Displayed Good Precision
Precision (repeatability) was evaluated by analyzing the assay control sample independently diluted eight times in a single analytical run (Figure 10). The Fab activity assay demonstrated acceptable repeatability with a 3.6% CV for the eight independently diluted control samples. Intermediate precision was determined by running the purified Fab control sample on 36 different occasions by two different operators from different laboratories. The average percent activity was 102% with a CV of only 5.3% for all runs (Figure 11).
|Repeatability (n = 8)|
|Avg = 97%|
|%CV = 3.6|
|Level||Number||Mean||Std dev||Std error mean||Lower 95%||Upper 95%|
|n = 36||% Activity||%CV|
The activity assay developed by Boehringer Ingelheim, Fremont, USA for their Fab molecule is currently being used for lot release and stability testing within the QC department. The assay has faster turn around times than ELISA and Biacore systems. The Fab activity assay is accurate and robust, with intermediate and intramediate precision less than 10%. Drug activity measurement using the Octet system has become a critical parameter for their product evaluation, and has resulted in increased Fab drug product consistency and quality. The activity data generated on the Octet system may be submitted to regulatory agencies for evaluation.