Reporter Genes

Reporter genes (or simply reporters) are genes that are typically attached to other genes of interest to monitor cellular events associated with gene expression, gene regulation, and signal transduction. Reporter gene expression is directly correlated to expression of the gene of interest. Certain reporters are commonly used as markers because they are easy to measure and offer high sensitivity.

Firefly luciferase, one of the most commonly used reporters, produces light by catalyzing a bioluminescence reaction that oxidizes D-luciferin in the presence of oxygen and ATP.

Luciferase from the sea pansy Renilla reniformis is often used in multiplexed luciferase assays, where it serves as a second reporter against which the firefly signal is normalized. Under the control of a constitutive promoter, Renilla luciferase signal provides a readout for cell number and transfection efficiency.


Application Notes



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*Dual luciferase assay kit only.
Readers must be equipped with injectors to use with either reporter gene assay kit.