Simplify characterization of neurons using automated cell imaging and preconfigured neurite outgrowth analysis protocol
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Analyze results for measurements of interest
View data using a heatmap with selected measurement values including cell count, total outgrowth, average processes, and average branches.
Pre-select wells of interest during acquisition set-up
Two wells of interest (a control vs. treated well) were selected to view at the same time during set-up of acquisition. Images were acquired using the ImageXpress Pico Automated Cell Imaging System.
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Quickly view trends across the plate
During acquisition, CellReporterXpress software allows users to quickly view trends across the plate using the thumbnail view.
Sort values to view your data of interest
The table analysis format can be used to analyze results for measurements of interest without having to look within individual wells, as you may need to in a heatmap. The table analysis format also allows users to apply the heatmap independently to each column of values.
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Visualize analysis of two wells of interest
Visualize the difference between the number and length of outgrowths with CellReporterXpress software using a mask overlay to compare between stimulated and unstimulated cells.
Visualize trends for measurements of interest
The scatter plot analysis format allows users to identify trends for measurements of interest against specific wells or other measurements. In this example, we see that the valproic acid treatment induces differentiation of neuroblastoma cells and that the control Neuro2a cells have fewer processes than D7 deficient Neuro2a cells in both treatments.
Latest Resources
Resources of Neurite Outgrowth
Application Note
Neurite outgrowth analysis in 3D reconstructed human brain micro-tissues
Neurite outgrowth analysis in 3D reconstructed human brain micro-tissues
Organotypic 3D cultures resemble native 3D tissue architecture and are believed more representative of real tissues than their 2D (monolayer) counterparts, thus providing higher in vivo…
Blog
7 tips for optimizing your 3D cell imaging and analysis workflow
7 tips for optimizing your 3D cell imaging and analysis workflow
As the field of cell biology moves toward increasing complexity of assays, 3D cell systems have proven to provide more physiologically relevant information as compared to…
eBook
Quantitate Neuron Potential
Quantitate Neuron Potential
Gain valuable insights into neurobiology, from evaluation of ion channels to analysis of 3D neuron organoids.
Scientific Poster
Neurite Tracing Application Module
Neurite Tracing Application Module
New in MetaMorph® NX Software 2.5, the Neurite Tracing Application Module simplifies the meticulous tasks associated with neuronal research. For simple analyses, quickly segment, then measur…
Scientific Poster
Development and Characterization of Human iPSC-derived Neurons for Drug Discovery Applications
Development and Characterization of Human iPSC-derived Neurons for Drug Discovery Applications
The human brain represents a complex organ that has consistently been proven difficult to model in vitro. Current models including primary rodent tissue and immortalized cell lines have serv…
Scientific Poster
Accelerating Your High Content Imaging: A Case Study Using Neuronal Toxicity Screening
Accelerating Your High Content Imaging: A Case Study Using Neuronal Toxicity Screening
By pairing induced pluripotent stem cells (iPSCs) of human origin with an automated high content imaging system, endpoint and live-cell assays can be used to determine whether pharmaceutical…
Scientific Poster
High Content Neuronal Toxicity Assays Using iPSC Derived Neurons
High Content Neuronal Toxicity Assays Using iPSC Derived Neurons
The nervous system is a target organ for the toxic effects of chemical compounds, environmental agents and some naturally occurring substances. Neurotoxicity can cause temporary or permanent…
Application Note
Measuring neurite outgrowth with the SpectraMax MiniMax cytometer and MetaMorph software
Measuring neurite outgrowth with the SpectraMax MiniMax cytometer and MetaMorph software
Neurons create connections via extensions of their cellular body called dendrites or “processes”. This biological phenomena is referred to as neurite outgrowth and is regulated by complex…
Application Note
High-content screening of neuronal toxicity using iPSC-derived human neurons
High-content screening of neuronal toxicity using iPSC-derived human neurons
The nervous system is sensitive to the toxic effects of many chemical compounds, environmental agents and certain naturally occurring substances. Neurotoxicity can cause temporary or…
Application Note
Intelligent time-lapse imaging to enhance live cell assay development
Intelligent time-lapse imaging to enhance live cell assay development
The ability to monitor responses in living cells over a specific period of time offers cell biologists several key advantages for assay development. For routine cellbased screening, time-…
Flyer
Neurite Outgrowth Application Module
Neurite Outgrowth Application Module
Explore the Neurite Outgrowth Application Module for MetaXpress software, which is designed for the analysis of neurite outgrowth assays
Scientific Poster
In Vitro Cardiotoxicity and Neurotoxicity Assessment of Environmental Chemicals Using Organotypic Human Induced Pluripotent Stem Cell-Derived Models
In Vitro Cardiotoxicity and Neurotoxicity Assessment of Environmental Chemicals Using Organotypic Human Induced Pluripotent Stem Cell-Derived Models
An increased prevalence of neurological and cardiovascular disorders, possibly attributed to environmental toxicant exposures, has generated an interest in developing reliable and efficient…
Scientific Poster
High-Throughput High-Content Screening for Developmental Neurotoxicity in Human iPSC-Derived Neurons
High-Throughput High-Content Screening for Developmental Neurotoxicity in Human iPSC-Derived Neurons
There is an increased need to develop reliable and efficient screening tools to identify chemicals with the potential to adversely affect neurological development. Human iPSC-derived neurons…
Scientific Poster
High-Content Assay for Morphological Characterization of Neuronal Development in 3D Matrix Using Human iPSC-Derived Neuronal Cultures
High-Content Assay for Morphological Characterization of Neuronal Development in 3D Matrix Using Human iPSC-Derived Neuronal Cultures
Development of more complex, biologically relevant, and predictive cell-based assays for compound screening is one of the main challenges in drug discovery. There is an increasing interest i…