Neurite Outgrowth Analysis

  • Gain valuable insight for interpreting neurotoxicity
  • Increase efficiency with real-time acquisition and analysis
  • Simplify characterization of neurons with preconfigured Neurite Tracing analysis protocol
  • Assess data quickly using integrated visualization tools
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Simplify characterization of neurons using automated cell imaging and preconfigured neurite outgrowth analysis protocol

  • Analyze results for measurements of interest

    ImageXpress Pico System with CellReporterXpress Software - Heat Map with Measurements

    View data using a heatmap with selected measurement values including cell count, total outgrowth, average processes, and average branches.

    Pre-select wells of interest during acquisition set-up

    ImageXpress Pico System with CellReporterXpress Software - Neuro2a cells at 10X

    Two wells of interest (a control vs. treated well) were selected to view at the same time during set-up of acquisition. Images were acquired using the ImageXpress Pico Automated Cell Imaging System. 

  • Quickly view trends across the plate

    ImageXpress Pico System with CellReporterXpress Software - Thumbnail View of Neurite Outgrowth

    During acquisition, CellReporterXpress software allows users to quickly view trends across the plate using the thumbnail view. 

    Sort values to view your data of interest

    ImageXpress Pico System with CellReporterXpress Software - Table Analysis

    The table analysis format can be used to analyze results for measurements of interest without having to look within individual wells, as you may need to in a heatmap. The table analysis format also allows users to apply the heatmap independently to each column of values.

  • Visualize analysis of two wells of interest

    ImageXpress Pico System with CellReporterXpress Software - Mask Overlay

    Visualize the difference between the number and length of outgrowths with CellReporterXpress software using a mask overlay to compare between stimulated and unstimulated cells.

    Visualize trends for measurements of interest

    ImageXpress Pico System with CellReporterXpress Software - Scatter Plot Analysis

    The scatter plot analysis format allows users to identify trends for measurements of interest against specific wells or other measurements. In this example, we see that the valproic acid treatment induces differentiation of neuroblastoma cells and that the control Neuro2a cells have fewer processes than D7 deficient Neuro2a cells in both treatments.

Latest Resources

Ananda Neurite Untangled

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Ananda Neurite Untangled

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Neurite outgrowth analysis in 3D reconstructed human brain micro-tissues

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Neurite outgrowth analysis in 3D reconstructed human brain micro-tissues

Organotypic 3D cultures resemble native 3D tissue architecture and are believed more representative of real tissues than their 2D (monolayer) counterparts, thus…
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Quantitate Neuron Potential

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Quantitate Neuron Potential

Gain valuable insights into neurobiology, from evaluation of ion channels to analysis of 3D neuron organoids.

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Neurite Tracing Application Module

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Neurite Tracing Application Module

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Development and Characterization of Human iPSC-derived Neurons for Drug Discovery Applications

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Simplified Imaging for Quantifying Biological Complexity of iPSC-Derived Cardiomyocytes and Neurons

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Simplified Imaging for Quantifying Biological Complexity of iPSC-Derived Cardiomyocytes and Neurons

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Accelerating Your High Content Imaging: A Case Study Using Neuronal Toxicity Screening

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Resources of Neurite Outgrowth

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Videos & Webinars

Ananda Neurite Untangled

Ananda Neurite Untangled

Simplified Imaging for Quantifying Biological Complexity of iPSC-Derived Cardiomyocytes and Neurons

Simplified Imaging for Quantifying Biological Complexity of iPSC-Derived Cardiomyocytes and Neurons

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