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Hybridoma Workflow for Antibody Discovery

Workflow for hybridoma generation and screening of large antibody libraries

Hybridoma technology is a method for mass-producing antibodies in a hybrid cell line generated from the fusion of antibody-producing B-cells with an immortalized myeloma cell line, now called a hybridoma cell. Because every B-cell produces a unique antibody, single-cell cloning of hybridomas can be used to generate a diverse library of unique monoclonal antibodies at a large scale, which are very frequently used in the prevention, diagnosis, and treatment of disease.

Workflow: Generation and screening of antibody libraries using hybridomas

Step 1: Fusion 

The process of fusing B cells, expressing unique antibodies, with myeloma cells creating a hybrid cell line is called a hybridoma.
 

Step 2: Clone screening 

The identification of clonally-derived hybridoma cell lines which are producing high amounts of monoclonal antibodies.
 

Step 3: Cell growth 

Cell growth is determined by monitoring cell divisions over a given period of time using label-free imaging.
 

Step 4: Specificity and cross reactivity  

The process of analyzing the ability of an antibody to bind to only one target antigen.
 

Step 5: Binding affinity and internalization 

Binding affinity is the strength of the binding interaction between a single biomolecule (e.g. protein or DNA) to its ligand/binding partner (e.g. drug or inhibitor). Internalization is the process of monitoring the ability of a given particle to enter into the cell.
 

 

Systems to accelerate your COVID-19 research

Get up and running rapidly with proven technologies

Molecular Devices is able to support your research needs by offering technology and solutions rapidly with express processing, shipping and customized finance solutions if needed on microplate readers, biopharma and cellular imaging systems.

AquaMax Microplate Washer

Our washers provide speed and configurability options—from single-tube dispensing to washing full 96- and 384-well plates.

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SpectraMax i3x Multi Mode Microplate Reader

Our SpectraMax® series of microplate readers measure absorbance, fluorescence, and luminescence plus additional detection modes with available upgrades for western blot, cell imaging, and fast kinetics with injectors. GMP/GLP labs can meet FDA guidelines with our SoftMax Pro GxP compliance and validation solution.

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Softmax Pro Gxp Software

SoftMax® Pro 7.1.1 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. Every step is optimized to simplify analysis and reporting to support our microplate readers.

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QPix 420 Microbial Colony Pickers

The QPix™ 400 Series Microbial Colony Pickers combine intelligent image analysis with precise automation for fast and efficient screening of large libraries. Capable of picking up to 3000 colonies per hour, it will streamline your workflow.

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ClonePix 2 Mammalian Colony Picker

The ClonePix™ 2 Mammalian Colony Picker is a fully automated system for the selection of high-value clones used in antibody discovery and cell line development.

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fsight

The CloneSelect™ Single-Cell Printer™ Series deposits single cells gently and with high efficiency using a patented, inkjet-like disposable, one-way dispensing cartridge.

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CloneSelect Imager

The CloneSelect™ Imager is a high-throughput automated solution for imaging and analyzing mammalian cells.

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ImageXpress Pico Automated Cell Imaging System

Our ImageXpress systems offer an end-to-end solution for high-content screening and analysis. All our systems support a wide range of applications, increased throughput, and streamlined workflows.

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Resources related to COVID-19 and viral research applications

Learn more about how our technology solutions can help support your research of COVID-19 cellular responses and vaccine development.

Videos and Demos

Hybridoma Selection using HAT Medium

Hybridoma selection using HAT medium

Preparing and Plating Cells in Semi Solid Medium

Preparing and Plating Cells in Semi-Solid Medium

XP Media and CloneMedia for Mouse Hybridoma Generation

XP Media and CloneMedia for Mouse Hybridoma Generation

Systems to accelerate your COVID-19 cellular response and vaccine development

We have validated and compliant laboratory solutions including microplate readers, microplate washers, biopharma and cellular imaging systems to meet your research needs.