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Cellular Signaling

Measure cellular responses occurring through a wide range of signaling pathways

Cellular signaling and cellular responses

Cellular signaling allows cells to respond to their environment as well as to communicate with other cells. Proteins located on the cell surface can receive signals from the surroundings and transmit information into the cell via a series of protein interactions and biochemical reactions that comprise a signaling pathway. Multicellular organisms rely upon an extensive array of signaling pathways to coordinate the proper growth, regulation, and functioning of cells and tissues. If signaling between or within cells is dysregulated, inappropriate cellular responses may lead to cancer and other diseases.

Many tools have been developed to measure cellular responses occurring through a wide range of signaling pathways. G protein-coupled receptor (GPCR) signaling can be studied using assays ranging from calcium flux, which can be monitored using fluorescence dyes, to changes in downstream effector molecules assessed by TR-FRET. Fluorescent molecules have been harnessed to reveal changes in the activity of kinases that play key roles in signaling pathways. Luciferase reporter gene assays utilizing luminescent reactions have become a versatile tool for studying gene regulation and cell signaling in both basic research and drug discovery.

Relevant applications and assays

Here, we present a variety of cell signaling applications that can be run using microplate readers with fluorescence, TR-FRET, fluorescence polarization, and luminescence detection modes.

Resources of Cellular Signaling