Measure live cells migrating over time with cell migration assays using automated imaging
The movement or migration of cells is often measured in vitro to elucidate the mechanisms of various physiological activities such as wound healing or cancer cell metastasis. Cell migration assays may be conducted in a controlled environment using live cell timelapse imaging. A "wound" in a confluent monolayer of cells growing in a microplate is created, either by manually creating a scratch or by utilizing special microplates that provide a uniform and reproducible cell-free zone. Monitor cell proliferation, migration, and spreading using transmitted light or live cell-compatible fluorescence. These medium to high throughput assays may be used to compare the migration between cells treated with either inhibitory or stimulatory compounds.
Use a simple scratch assay, with continuous or discontinuous timelapse acquisition, to evaluate wound healingWound healing can be assessed by acquiring images with a transmitted light or fluorescence microscope and quantifying using a third party analysis software but the throughput is low. For a higher-throughput microplate format, an automated imaging system paired with analysis software is a more efficient solution. Molecular Devices offers different options for automated imagers so that labs can select a system that best fits their research or screening needs.
Resources for Cell Migration Assays
The Custom Module Editor in version 5 of MetaXpress® Software is a flexible, interactive environment where users can create and test a template for image analysis. Galleries of processing…
Effectively image and analyze cell migration using a scratch assay or a dissolvable biocompatible gel
Cell migration is an essential process required for many biological events including embryonic development, wound healing, cancer metastasis, and immunological responses.
Download this document for analysis of the Automatable 3 ‐Dimensional Cell Invasion Assay using ImageXpress Micro Widefield HCS System and MetaXpress and AcuityXpress Software.
Cell migration, the movement of cells from one location to another, is a critical component of both normal and abnormal biological processes. The importance of cell migration in diverse…
Videos and Demos
Make movies from timelapse images
Acquire a discontinuous time-lapse video on ImageXpress Micro
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