MultiWash III

The MultiWash III Microplate Washer is a low-cost, 96-well microplate washer that represents the perfect balance between features and affordability

The MultiWash III Washer can be used to perform a wide range of assays including enzyme-linked immunosorbent assay (ELISA), enzyme immuno assay (EIA), fluorescent immuno assay (FIA), luminescent immuno assay (LIA), cytotoxicity assays, and agglutination tests in flat, round, or V-bottom microplates. The system is completely self-contained with no external pumps, and can be assembled and fully operational in just minutes. Once operational, the MultiWash III Washer can perform a 300 µL three-cycle wash on a 96-well plate in less than two minutes. The unique coaxial manifold design combines the dispense and aspiration channels in one probe. This allows the user to wash with greater speed than typical designs that have separated aspiration and dispense channels. The modular manifold design also allows the user to wash in either column or row format depending on the manifold installed.

User-Changeable Wash Manifold for Different Assays

  • There are two main manifold options, the innovative Coaxial Manifold and the Standard Manifold with separated aspiration and dispense pins
  • Each manifold type is available in 8 channels, 12 channels, and 8 x 2 channels
  • The coaxial manifolds allow for bottom-washing and overflow washing for Biochemical and Cell-Based Assays
  • Manifolds can be changed in minutes
  • Buy the manifolds you need now and add additional manifold as your needs change
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Figure 1Coaxial needle
multiwashIII-overflow-washing.jpg
Figure 2. Bottom and overflow washing

Simple and Flexible Operation

  • Color coded quick connect fittings on bottles allow users to easily change solutions.
  • The soft-touch keys on the display panel are used to create and store up to 50 user-defined programs.
  • Wash 1 or more strips, or entire plate
  • Dispense volumes from a range of 50-1990 µL in row, column, or double-column format depending on Manifold installed.
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Figure 3. Color-coded fittings
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Figure 4. Front panel display pad

Dependable Operation

  • Reliable positive-displacement dispense pump that assures accurate and consistent dispensing
  • Aspiration from the microplate wells is facilitated by the use of a built-in vacuum pump, eliminating the need for additional equipment
  • To ensure reliability, the instrument performs a maintenance rinse after every eight hours of non-usage to prevent wash solutions from drying up and leaving salt deposits between uses
  • The waste reservoir is fitted with a liquid level sensor to indicate if the reservoir is full
  • Only the tips of the aspiration needles make contact with liquid in the microplate wells, so cross-contamination and carry-over are minimized

The MultiWash III Washer is geared to users that have lower throughput applications, but still want the benefits of a microplate washer. Any assay that needs washing as a primary, intermediary, or final step before quantitation is amenable to be processed on the MultiWash III Washer. Some examples are

  • ELISA (Enzyme-Linked ImmunoSorbent Assay)
  • FIA (Fluorescent Immuno Assays)
  • LIA (Luminescent Immuno Assay)
  • Cytotoxicity assays
  • Agglutination assays
  • Cell-based assays

The MultiWash III Washer is programmed using the easy to use front display panel. No external computer or software is necessary. The soft-touch keys on the display panel (Figure 2) are used to create and store up to 50 user-defined programs. All functions including single-button cleaning are accessed from the front panel.  Precise dispense and aspiration control allows both fast processing of robust biochemical assays and gentle processing of cell-based assays utilizing either the bottom wash or top wash settings.

multiwashIII-front-panel.jpg

Performance Specifications

Resolution
10 µL
Accuracy
±3% @ 300 µL
Precision
3% CV @ 300 µL
Processing time (full plate, 3 cycles, 300 µL/well)
8-port manifold:  180 sec
12-port manifold:  120 sec
2- to 8-port manifold:  90 sec
Dispense volume per well
8-port manifold:  50-1990 µL
12-port manifold:  50-1650 µL
2- to 8-port manifold:  50-1250 µL
Residual aspiration volume
<3 µL per well
Maximum soak time
999 sec.
Maximum wash cycles
10
Liquid contact materials
Delrin®, Glass, Nylon, Polypropylene Tygon®, Teflon®, Polyethylene, Santoprene, and Stainless Steel

General Specifications

Dimensions (in.)
9.5 (H) x 11 (W) x 14.5 (D)
Dimensions (cm)
24 (H) x 28 (W) x 37 (D)
Weight (instrument)
12 lbs.    5.4 kg
Power requirements
99-132 Vac/198-264 Vac, 50-60 Hz
Power consumption
30 watts max.
Operating temperature
10-40°C
Storage temperature
1-40°C
Fluid reservoir capacity
2 L each for Wash, Rinse, and Waste
MultiWash III Microplate Washer
 

Product

Part Number

MULTIWASH III SYSTEM WITH 8-PORT CO-AXIAL MANIFOLD
9000-0923
MULTIWASH III SYSTEM WITHOUT MANIFOLD
9000-1076
8-PORT STANDARD MANIFOLD FOR MULTIWASH III
9000-1077
12-PORT STANDARD MANIFOLD FOR MULTIWASH III
9000-1006
(2) 8-PORT STANDARD MANIFOLD FOR MULTIWASH III
9000-1078
8-PORT CO-AXIAL MANIFOLD FOR MULTIWASH III
9000-0926
12-PORT CO-AXIAL MANIFOLD FOR MULTIWASH III
9000-0927
(2) 8-PORT CO-AXIAL MANIFOLD FOR MULTIWASH III
9000-0928
Accessories
 
MAINTENANCE KIT AND SYRINGE FOR MULTIWASH III
9000-0929
REPLACEMENT SYRINGE FOR MULTI WASH III (#945)
9000-0930
IN-LINE FLUID FILTERS FOR MULTIWASH III (10/PK) (#509)
9000-0504
AEROSOL SHIELD FOR MULTIWASH III
9000-0128