New Video: Import and analyze data from any instrument  Watch Video >>

FREE 2-Week SoftMax Pro Software Trial - Try it today.

SoftMax Pro Data Acquisition and Analysis Software

From passions to progress

Transform your brilliant ideas and intriguing questions into data, meaningful analyses, visualization, collaboration, communication, and more. SoftMax® Pro Software is the most published microplate reader control and microplate data analysis software package available. With over 140 ready-to-run protocols for our microplate systems, 19 different curve fit options, analysis algorithms, third-party data import options, a collaborative worldwide user community, a variety of presentation ready data output formats, and built-in validation and compliance solutions, SoftMax Pro Software is there for you at every step, helping you transform your passions into progress.

Set up assays within minutes
  • Use over 140 pre-written protocols with pre-configured assay parameters and analysis settings to read, analyze, and report without having to become an expert
  • Acquire the best data for your assay using several read options including endpoint, kinetic, spectral scan, and well scan
  • Supports traditional and unconventional microplate reader assays and novel modes, including imaging cytometry and Western blots
  • Import data in microplate format into SoftMax Pro Software from any scientific instrument
  • Import XML-based data directly into SoftMax Pro Software for easy automation
Streamline analysis and automation
  • Utilize one of the 19 different curve fit options to fit and graph your data in the best possible way
  • Apply standards across multiple plates to simplify cross-plate analysis and interpolation
  • Perform custom, mathematical calculations with the SoftMax Pro formula system and Syntax Helper
  • Create reports your way with customizable notes sections and even include your own logo
  • Export directly to a .xls (Excel) file or automate data export to a LIMS
  • Easily save as a PDF file

Comprehensive reader validation tools are available for laboratories working under GLP, GMP, 21 CFR Part 11 and other regulated environments.

Combining the all-in-one functionality of SoftMax Pro Data Acquisition & Analysis Software for microplate readers with tools for speeding compliance for GLP and GMP environments.

SoftMax Pro GxP Compliance Software extends Molecular Devices leading data acquisition and analysis solution into regulated laboratories working under GMP, GLP, 21 CFR Part 11, and other similar guidelines for secure electronic records. 


  • Extensive suite of tools available for validation can reduce the cost and time of validation as compared to using multiple platforms to collect and analyze data by 50%
  • Provides end-to-end chain of custody from capture through analysis to validation of data
  • Eliminates extra time and loss of accuracy associated with transfer between software platforms
  • Support from a single vendor that develops both hardware and software offering over 140 specialized protocols for analyzing microplate data. Robust turnkey solution saves up to $200K in method development cost vs alternatives


  • SoftMax Pro 6 GxP is our 3rd generation software with compliance tools
  • Over 100 field and internal staff with full understanding of hardware, applications, and analysis with over a century of combined experience
  • More than 120,000 software licenses sold since 2004
  • Satisfied customers include all 50 of the top 50 global pharmaceutical companies

Secure, traceable electronic record-keeping

  • Controlled user access through a granular permission structure and unique logins
  • Electronic signature support for verification, authorization, and approval
  • Audit trails to document the history of user actions for each data file
  • Local and remote administration of user accounts for straightforward deployment

Compatible with comprehensive reader validation tools

SoftMax Pro Software includes ready-to-use templates for analysis of assays run on Molecular Devices microplate readers, including:

  • ADME-Tox
  • Cardiotoxicity assays
  • Cell adhesion
  • Cell migration
  • Cell viability, proliferation, and cytotoxicity
  • ELISAs and immunoassays
  • Endotoxin detection
  • Enzyme assays
  • Epigenetics assays
  • Fatty acid uptake
  • Imaging cytometry assays
  • Low Volume DNA quantitation
  • Microbial growth
  • Neurotransmitter transporter uptake
  • Nucleic acid quantitation
  • Phosphatases/kinases
  • Protein quantitation
  • Reporter gene assays
  • Western blotting

SoftMax Pro Software version 6 can be installed on a computer with the following system requirements.




Processor Single-core, 2 GHz or faster Quad core or faster
Operating system Window 7, 32-bit or 64-bit (x86 or x64) and .NET Framework 4.0 (.NET Framework is installed automatically by the SoftMax Pro Software installer if necessary) Windows 7, 32-bit or 64-bit (x86 or x64), or Windows 8, 32-bit or 64-bit (x86 orx64)
Data Connection RS-232 serial port or USB 2.0 port (depending on the instrument) USB 2.0 ports; For those that require an RS-232 serial port, you can use a USB 2.0 port with a Keyspan, USB-to-serial adapter. Keyspan USB-to-serial adapters have been field tested and given approval by Molecular Devices.
Memory 2 GB RAM 4GB RAM; If running on a virtual machine, Molecular Devices recommends a minimum of 6 GB RAM. For automation, Molecular Devices recommends a minimm of 8 GB RAM
Hard Disk 500 MB of available space 1 GB of available space or more
Graphics Display Graphics display adapter 1024 x 768 or higher-resolution display 32-bit graphics display with 265 MB video RAM; 1280 x 1024 or higher resolution display
Software installation and activation CD/DVD drive; Internet connection or external USB drive CD/DVD drive; Internet connection

Helpful Tips:

  • To prevent data loss, turn off all sleep and hibernation settings for the hard disk, the CPU, and the USB ports
  • Disable automatic Windows Updates
  • Update WIndows manually when the instrument isn't being used by the software; these options can be enabled in Windows Control Panel

Note: Installing and using the SoftMax Pro Software on the Windows XP operating system is no longer supported. The software is neither tested nor validated on Windows XP.

SoftMax Pro Software Trial Download

Related Videos

Watch the 3-minute "SoftMax Pro 6.4.1 Import Feature" video.
Watch the 7-minute "Getting Started with SoftMax Pro 6.4" webinar.
Watch the Webinar: SoftMax Pro GXP Compliance Software - Compliance without Complications.

Related Collateral



Data Sheets

Related Webinars






Annual Subscription License

Non-Expiring License

SoftMax Pro Standard Software

Windows 7/8 compatible

One year subscription to the latest version of SoftMax Pro 6. Includes one activation key, which expires after one year.  Annual renewal.

Part number: (Click below to purchase/download)

*For verified academic institutions

Non-expiring license for the latest version of SoftMax Pro 6. Includes four activation keys.

Part number: (Click below to purchase/download)

*For verified academic institutions

SoftMax Pro GxP Software

Windows 7/8 compatible


Latest version of SoftMax Pro 6 GxP. Includes tri-fold CD Package with GxP Admin, SoftMax Pro 6 GxP, and Validation Pack CDs, GxP User License certificate, SoftMax Pro 6 GxP Product Activation Key Sheet (three activations pre user), and Compliance certificate.

Software part number:

User license part number:
SMP6 GXP License (3-10)
SMP6 GXP License (11-50)
SMP6 GXP License (>50)

*Requires purchase of any amount of user licenses (minimum three). Tiered pricing is based on number of user licenses purchased. Please select one user license part number when ordering.

SoftMax Pro Importer XLS

Requires SoftMax Pro 6.4.1 or higher

One year subscription to enable import of data into SoftMax Pro from Excel based template.

Includes one activation key, which expires after one year.  Annual renewal.

Part number: (Click below to purchase/download)


SoftMax Pro Importer XLS and XML

Requires SoftMax Pro 6.4.1 or higher

One year subscription to enable import of data into SoftMax Pro from Excel based template or XML file.

Includes one activation key, which expires after one year.  Annual renewal.

Part number: (Click below to purchase/download)


For previous versions or for multiple user licenses, please contact your regional sales manager.