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SoftMax Pro Data Acquisition and Analysis Software

Set-up, read and analyze your microplate data faster

Industry-leading SoftMax® Pro 6 Software is used by over 100,000 users worldwide, delivering the most comprehensive portfolio of ready-to-run protocols, analysis algorithms and FDA 21 CFR Part 11 compliance tools to streamline your microplate reader applications.

Set-up assays within minutes

  • Over 140 ready-to run protocols
  • Supports traditional and unconventional microplate reader assays, including imaging cytometry and western blots
  • Protocol sharing through >100,000 user community
  • Spectral optimization wizard to maximize assay signal window
  • Patented PathCheck® Technology to automate OD well volume normalization
  • Automatic instrument recognition of Molecular Devices microplate readers

Streamline analysis and automation

  • Syntax Helper simplifies sophisticated analysis: EC/IC50 calculations to tests for parallelism, relative potency, non-linear confidence intervals, and statistics across groups, single or multiple plate data sets
  • Enhanced curve fitting for complex data sets, including 4 and 5-parameter logistic curve fits
  • Automated analysis and results flagging for routine assays
  • Real-time data display of kinetic measurements and spectral scans
  • Walk-away automation through proven robotic and LIMS partner integrations
  • Comprehensive reader validation tools ideal for laboratories working under GLP, GMP, 21 CFR Part 11 and other regulated environments

Combining the all-in-one functionality of SoftMax Pro Data Acquisition & Analysis Software for microplate readers with tools for speeding compliance for GLP and GMP environments.

SoftMax Pro GxP Compliance Software extends Molecular Devices leading data acquisition and analysis solution into regulated laboratories working under GMP, GLP, 21 CFR Part 11, and other similar guidelines for secure electronic records. 


  • Extensive suite of tools available for validation can reduce the cost and time of validation as compared to using multiple platforms to collect and analyze data by 50%
  • Provides end-to-end chain of custody from capture through analysis to validation of data
  • Eliminates extra time and loss of accuracy associated with transfer between software platforms
  • Support from a single vendor that develops both hardware and software offering over 140 specialized protocols for analyzing microplate data. Robust turnkey solution saves up to $200K in method development cost vs alternatives


  • SoftMax Pro 6 GxP is our 3rd generation software with compliance tools
  • Over 100 field and internal staff with full understanding of hardware, applications, and analysis with over a century of combined experience
  • More than 120,000 software licenses sold since 2004
  • Satisfied customers include all 50 of the top 50 global pharmaceutical companies

Secure, traceable electronic record-keeping

  • Controlled user access through a granular permission structure and unique logins
  • Electronic signature support for verification, authorization, and approval
  • Audit trails to document the history of user actions for each data file
  • Local and remote administration of user accounts for straightforward deployment

Compatible with comprehensive reader validation tools

SoftMax Pro Software includes ready-to-use templates for analysis of assays run on Molecular Devices microplate readers, including:

  • ADME-Tox
  • Cardiotoxicity assays
  • Cell adhesion
  • Cell migration
  • Cell viability, proliferation, and cytotoxicity
  • ELISAs and immunoassays
  • Endotoxin detection
  • Enzyme assays
  • Epigenetics assays
  • Fatty acid uptake
  • Imaging cytometry assays
  • Low Volume DNA quantitation
  • Microbial growth
  • Neurotransmitter transporter uptake
  • Nucleic acid quantitation
  • Phosphatases/kinases
  • Protein quantitation
  • Reporter gene assays
  • Western blotting

SoftMax Pro Software version 6 can be installed on a computer with the following system requirements.




Processor Single-core, 2 GHz or faster Quad core or faster
Operating system Window 7, 32-bit or 64-bit (x86 or x64) and .NET Framework 4.0 (.NET Framework is installed automatically by the SoftMax Pro Software installer if necessary) Windows 7, 32-bit or 64-bit (x86 or x64), or Windows 8, 32-bit or 64-bit (x86 orx64)
Data Connection RS-232 serial port or USB 2.0 port (depending on the instrument) USB 2.0 ports; For those that require an RS-232 serial port, you can use a USB 2.0 port with a Keyspan, USB-to-serial adapter. Keyspan USB-to-serial adapters have been field tested and given approval by Molecular Devices.
Memory 2 GB RAM 4GB RAM; If running on a virtual machine, Molecular Devices recommends a minimum of 6 GB RAM. For automation, Molecular Devices recommends a minimm of 8 GB RAM
Hard Disk 500 MB of available space 1 GB of available space or more
Graphics Display Graphics display adapter 1024 x 768 or higher-resolution display 32-bit graphics display with 265 MB video RAM; 1280 x 1024 or higher resolution display
Software installation and activation CD/DVD drive; Internet connection or external USB drive CD/DVD drive; Internet connection

Helpful Tips:

  • To prevent data loss, turn off all sleep and hibernation settings for the hard disk, the CPU, and the USB ports
  • Disable automatic Windows Updates
  • Update WIndows manually when the instrument isn't being used by the software; these options can be enabled in Windows Control Panel

Note: Installing and using the SoftMax Pro Software on the Windows XP operating system is no longer supported. The software is neither tested nor validated on Windows XP.

SoftMax Pro Software Trial Download

Related Videos

Watch the 7-minute "Getting Started with SoftMax Pro 6.4" webinar.
Watch the Webinar: SoftMax Pro GXP Compliance Software - Compliance without Complications.

Related Collateral

Data Sheets

Related Webinars






Licensing structure

SoftMax Pro Software 

  • Version 6.4
  • 5-200 user license
  • Full site user license
  • Full global user license
  • Extension licenses sold separately
SoftMax Pro GxP Software

  • Version 6.4
  • Version 5.4
  • 5-200 user license
  • Full site user license
  • Full global user license
  • Extension licenses sold separately
All license packages (except extension licenses) include:
  • SoftMax Pro GxP Software CD
  • GxP Admin Software CD
SoftMax Pro Software Validation Package (for > 10 user licenses)
SoftMax Pro Software Maintenance Plan
  • Free feature packaged upgrades (6.X to 6.Y) for SoftMax Pro Software automatically available to customers
  • Significant discount on SoftMax Pro (major upgrades from 6.X to 7.X)
  • Additional license seats

For previous versions or for multiple user licenses, please contact your regional sales manager.