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SpectraMax Paradigm Multi-Mode Microplate Reader

A compact, capable, and user-upgradeable microplate reader that enables you to do more, with less.

Future-proof your lab with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. Get the system that delivers the data you need today, and when you need more, transform your reader with a detection-mode addition upgrade that takes less than two minutes. The SpectraMax Paradigm reader can do absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence measurements. Detection cartridge modularity allows you to configure the system to meet your current needs, while providing flexibility to address future applications.

Benefits

  • Flexible design allows for single or dual excitation and emission, to address existing needs and future expansion
  • User upgradable microplate reader in < 2 minutes
  • High speed detection
  • Excellent sensitivity and performance
  • Applications at your fingertips

Features

  • Array of detection cartridge capabilities
  • Automatic Z-height optimization (both top and bottom read configurations)
  • Fluorescence measurements no longer require you to adjust the gain
  • Patented LED intensity adjustment
  • Up to 1536-well format
  • Temperature control
  • Monochromator-based absorbance
  • Filter-based fluorescence
  • TUNE Cartridge available for unmatched sensitivity in wavelength tunable detection
  • Custom Cartridge development available to meet your specific research needs
  • SpectraDrop™ Plate protocols enabling micro volume applications measurement are included
  • Seamlessly integrate with StakMax® Microplate Handling Stacker through SoftMax® Pro Software interface

Assay Kits

  • We offer optimized assay kits for use with the SpectraMax Paradigm reader.

SpectraMax® Paradigm® features future-ready applications that can be installed and ready to use in less than two minutes. With over 20 user-installable detection cartridges available, including custom cartridges, users can easily expand their microplate reader to meet their current and future research needs.

  • Fluorescence Intensity
    • DNA/RNA/protein quantitation
    • Endpoint qPCR
    • Green fluorescent protein
    • Receptor-ligand binding
  • Fluorescence Polarization
    • SNP genotyping
    • Protein-protein interactions
    • Drug receptor studies
  • Absorbance
    • DNA/RNA and protein purity
    • ELISA
    • Cell viability
    • Enzyme kinetics
    • Low volume applications
  • Time-Resolved Fluorescence
    • Enzyme Activity
    • TR-FRET Eu+3 chelates
    • Protein-protein interactions
    • Western blot detection
  • Glow Luminescence
    • LIA
    • Reporter gene
    • cAMP
    • Toxicity
    • Screens
    • Kinetics

SoftMax® Pro Microplate Data Acquistion & Analysis Software, Molecular Devices industry leading all-in-one data acquisition and analysis software comes standard with the Paradigm Platform. SpectraDrop™ Plate protocols that enable Micro Volume applications measurement are also included.

Benefits

  • The most extensive library of "Ready-to-Run" application protocols to get you started faster
  • Advanced calculations including non-linear fitting metrics for in-depth analysis
  • Syntax Helper to simplify complex calculations
  • Tools to customize reporting and exporting of data
  • GxP support for 21 CFR Part 11 compliance is available

 

softmaxpro-sw-v5_450_0.jpg

 

 

 

 

General Specifications

Light source
High Powered LED or Flash Lamp
Plate formats
6- to 1536-well
Temperature range
4°C above ambient to 45°C
Uniformity
± 0.75°C
Shaking
Linear, Orbital
Endpoint measurement
All Modes
Kinetic measurement
All Modes
Well area scanning
All Modes
Wavelength scanning
Photometry
Dimensions W Ã-H Ã-D cm (in.)
39 (15.4) -45 (17.7) -63 (24.8)
Weight, without cartridges
45 kg (99 lbs)
Power source
100-240 VAC, 2 A, 50/60 Hz
Robotic compatible
Yes
Linear dynamic range
> 5 decades

Technical Specifications

Fluorometry (FI) (Fluorescein)
Guaranteed*
Optimized
96-well (200 μL)
0.100 fmol
0.020 fmol
384-well (75 μL)
0.100 fmol
0.012 fmol
1536-well (8 μL)
0.030 fmol
0.003 fmol
Fluorescence Polarization (FP) (Fluorescein 1 nM, SD)
96-well (200 μL)
3.0 mP
0.5 mP
384-well (75 μL)
3.0 mP
0.5 mP
1536-well (8 μL)
6.0 mP
1.5 mP
Time Resolved Fluorescence (TRF) (Europium)
96-well (200 μL)
10.0 amol
2.5 amol
384-well (75 μL)
5.0 amol
1.0 amol (100 μL)
1536-well (8 μL)
1.5 amol
0.6 amol
Luminescence (Lum) (ATP-Glow)
96-well (200 μL)
0.50 fmol
0.30 fmol
384-well (50 μL)
0.50 fmol
0.30 fmol
1536-well (8 μL)
0.20 fmol
0.15 fmol
 * For properly functioning, operated and maintained equipment.
 
Photometric
Wavelength Range
230-1000 nm
Wavelength Selection
Monochromator (1.0 nm increments)
Wavelength Bandwidth
4.0 nm
Wavelength Accuracy
± 1 nm
Wavelength Repeatability
± 0.5 nm
Photometric Range
3.5 OD
Photometric Resolution
0.0001 OD
Photometric Accuracy
± (2% + 0.010 OD), 0-2 OD @ 405 nm
Photometric Precision
± (1% + 0.005 OD), 0-2 OD @ 405 nm

Related Videos

This video features the SpectraMax Paradigm Multi-Mode Microplate Reader with the TUNE Cartridge.

 

 

 

 

 

Related Collateral

Brochures/Datasheets

Applications

Posters

Related Webinars

 

 

SpectraMax® Paradigm Multi-Mode Microplate Reader

Product

Part Number

SpectraMax Paradigm Multi-Mode Microplate Reader with SoftMax Pro Software PARADIGM
SpectraMax Absorbance (ABS) Detection Cartridge
0200-7000
SpectraMax Tunable Wavelength (TUNE) Detection Cartridge
0200-7050
SpectraMax Multi-mode (MULTI) Detection Cartridge
0200-7001
SpectraMax Fluorescence Intensity (FI) (coum-fluor) Detection Cartridge
0200-7002
SpectraMax Fluorescence Intensity (FI) (fluor-rhod) Detection Cartridge
0200-7003
SpectraMax Fluorescence Intensity (FI) (Cy3-Cy5) Detection Cartridge
0200-7004
SpectraMax Fluorescence Intensity (FI) (CFP-YFP) Detection Cartridge
0200-7005
SpectraMax Fluorescence Intensity (FI) GeneBLAzer Detection Cartridge
0200-7006
SpectraMax Fluorescence Intensity Dual Label (FI-DL) (MultiTox-Fluor) Detection Cartridge
0200-7007
SpectraMax Time Resolved Fluorescence (TRF) Detection Cartridge
0200-7008
SpectraMax Fluorescence Polarization (FP) (fluorescein) Detection Cartridge
0200-7009
SpectraMax Fluorescence Polarization (FP) (rhodamine) Detection Cartridge
0200-7010
SpectraMax Cisbio HTRF Detection Cartridge
0200-7011
SpectraMax Glow Luminescence (LUM) Detection Cartridge
0200-7012
SpectraMax Dual Color Luminescence (LUM) (Chroma-Glo) Detection Cartridge
0200-7013
SpectraMax  Glow Luminescence (LUM) (96) Detection Cartridge
0200-7014
SpectraMax Glow Luminescence (LUM) (384) Detection Cartridge
0200-7015
SpectraMax Dual Color Luminescence (LUM) (BRET2TM) Detection Cartridge
0200-7016
SpectraMax AlphaScreen 384 STD Detection Cartridge
0200-7017
SpectraMax AlphaScreen 384 HTS Detection Cartridge
0200-7018
SpectraMax AlphaScreen 1536 HTS Detection Cartridge
0200-7019
 

Other Accessories

 Product Name
Part Number
SpectraDrop™ Micro-Volume Microplate Starter Kit
0200-6262
SpectraDrop™ Micro-Volume Microplate HTS Kit
0200-6267
Multi-Mode Validation Plate for SpectraMax® Paradigm® & FilterMax™ F3/F5 Systems
0200-7200

Number of Citations*: 2,730

Latest Citations:
For a complete list, please click here.

Artemether exhibits amoebicidal activity against Acanthamoeba castellanii through inhibition of the serine biosynthesis pathway

Y Deng, W Ran, S Man, X Li, H Gao… - Antimicrobial agents …, 2015 - Am Soc Microbiol
... 178 for an additional 4 h before reading the plates (28). Fluorescence was measured
on a SpectraMax 179 Paradigm Multi-Mode Microplate Reader (Molecular Devices,
USA) using a fluorescence 180 excitation wavelength ...
 
 

A Novel Paradigm for the Sex Differences in Response to Ultraviolet Radiation Exposure

E Sommer - 2015 - kb.osu.edu
Page 1. A Novel Paradigm for the Sex Differences in Response to Ultraviolet Radiation
Exposure An Undergraduate Honors Thesis ... reaction. The brown color produced by the reaction
was read by the SpectraMax plate reader over the course of 5 min. ...
 
 

[PDF] IL-15-activated NK cells kill autologous osteoclasts via LFA-1, DNAM-1 and TRAIL, and inhibit osteoclast-mediated bone erosion in vitro.

S Feng, SH Madsen, NN Viller, AV Neutzsky-Wulff… - …, 2015 - researchgate.net
... measuring the absorbance at 405 nm with 650 nm as reference using a microplate reader
SpectraMax Paradigm (Molecular Device). Quantification of cell viability by Presto Blue ... 535 nm
and emission at 595 nm using the SpectraMax Paradigm. TRAP staining ...
 

Interleukin‐15‐activated natural killer cells kill autologous osteoclasts via LFA‐1, DNAM‐1 and TRAIL, and inhibit osteoclast‐mediated bone erosion in vitro

S Feng, SH Madsen, NN Viller… - …, 2015 - Wiley Online Library
... The reaction was stopped with 0·3 m sodium hydroxide and quantified by measuring the
absorbance at 405 nm with 650 nm as reference using a microplate reader SpectraMax Paradigm
(Molecular Devices, Sunnyvale, CA). Quantification of cell viability by Presto Blue. ...
 
 

基于 DNA 的比色分析法快速测定尿液中的 Hg 2+

秋伶赵, 玲玲刘, 丽娜杨, 振宇张 - 分析化学, 2015 - sciencemeta.com
... 2. 1摇仪器与试剂 SpectraMaxParadigm襆 多功能酶标仪(美国Molecular Devices
公司);UV鄄2550 紫外分光光度计(日 本岛津公司);台式冷冻恒温摇床(太仓市实验设备厂);
PHS鄄3C 型pH 计(上海雷磁仪器厂)。 DNA 序列A (5忆鄄Biotin鄄ATCATGGACGTGCGTGTGAC ...
 
 

Microbiome structure of the fungid coral Ctenactis echinata aligns with environmental differences

C Roder, T BayerM Aranda, M Kruse… - Molecular …, 2015 - Wiley Online Library
... 10 min. For each sample, amplifications were performed in triplicate and combined. PCR
products for all samples were quantified using a microplate reader (SpectraMax Paradigm,
Molecular Devices) and the Qubit Broad Range assay (Invitrogen) prior to ...
 

High-sensitive and high-efficient biochemical analysis method using a bionic electronic eye in combination with a smartphone-based colorimetric reader system

K Su, Q Zou, J Zhou, L Zou, H Li, T Wang, N Hu… - Sensors and Actuators B …, 2015 - Elsevier
... Phosphate buffered saline (0.1 M PBS, pH 7.4) are procured from Invitrogen, Germany, while Tween
20 is from Carl Roth GmbH, Germany. MTPR is SpectraMax Paradigm (Molecular Devices, USA).
The smartphone is iPhone 4s and the pad is iPad 3 (Apple, USA). ...
 

Silver nanoparticle-embedded polymersome nanocarriers for the treatment of antibiotic-resistant infections

BM Geilich, AL van de Ven, GL Singleton… - Nanoscale, 2015 - pubs.rsc.org
... Control treatments were given 100 μL of PBS to keep the media dilution consistent. The well
plate was then allowed to incubate at 37 °C inside a spectrophotometer under static conditions
(SpectraMax Paradigm, Molecular Devices, Sunnyvale, CA). ...
 

Improved ethanol production from xylose in the presence of acetic acid by the overexpression of the HAA1 gene in Saccharomyces cerevisiae

Y Sakihama, T Hasunuma, A Kondo - Journal of bioscience and …, 2015 - Elsevier
... OD600). Aerobic batch cultivations were performed at 120 rpm, 30°C, for 96 h.
Growth was monitored by measuring the culture OD600 using a SpectraMax
Paradigm (Molecular Devices, Tokyo, Japan). Xylose fermentation. ...
 

Acetaminophen induces JNK/p38 signaling and activates the caspase-9-3-dependent cell death pathway in human mesenchymal stem cells

GT Yiang, YL Yu, KT Lin, JN Chen… - International …, 2015 - spandidos-publications.com
... After 3 h incubation at 37˚C, the absorbance (570 nm) was measured under a
multi-well enzyme-linked immunosorbent assay reader (SpectraMax Paradigm
Multi-Mode Microplate Reader; Molecular Devices, Sunnyvale, CA, USA). ...
 

 

 

* as of June 26, 2015.  Source: Google Scholar.