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SpectraMax Paradigm Multi-Mode Microplate Reader

A compact, capable, and user-upgradeable microplate reader that enables you to do more, with less.

Future-proof your lab with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. Get the system that delivers the data you need today, and when you need more, transform your reader with a detection-mode addition upgrade that takes less than two minutes. The SpectraMax Paradigm reader can do absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence measurements. Detection cartridge modularity allows you to configure the system to meet your current needs, while providing flexibility to address future applications.


  • Flexible design allows for single or dual excitation and emission, to address existing needs and future expansion
  • User upgradable microplate reader in < 2 minutes
  • High speed detection
  • Excellent sensitivity and performance
  • Applications at your fingertips


  • Array of detection cartridge capabilities
  • Automatic Z-height optimization (both top and bottom read configurations)
  • Fluorescence measurements no longer require you to adjust the gain
  • Patented LED intensity adjustment
  • Up to 1536-well format
  • Temperature control
  • Monochromator-based absorbance
  • Filter-based fluorescence
  • TUNE Cartridge available for unmatched sensitivity in wavelength tunable detection
  • Custom Cartridge development available to meet your specific research needs
  • SpectraDrop™ Plate protocols enabling micro volume applications measurement are included
  • Seamlessly integrate with StakMax® Microplate Handling Stacker through SoftMax® Pro Software interface

Assay Kits

  • We offer optimized assay kits for use with the SpectraMax Paradigm reader.

SpectraMax® Paradigm® features future-ready applications that can be installed and ready to use in less than two minutes. With over 20 user-installable detection cartridges available, including custom cartridges, users can easily expand their microplate reader to meet their current and future research needs.

  • Fluorescence Intensity
    • DNA/RNA/protein quantitation
    • Endpoint qPCR
    • Green fluorescent protein
    • Receptor-ligand binding
  • Fluorescence Polarization
    • SNP genotyping
    • Protein-protein interactions
    • Drug receptor studies
  • Absorbance
    • DNA/RNA and protein purity
    • ELISA
    • Cell viability
    • Enzyme kinetics
    • Low volume applications
  • Time-Resolved Fluorescence
    • Enzyme Activity
    • TR-FRET Eu+3 chelates
    • Protein-protein interactions
    • Western blot detection
  • Glow Luminescence
    • LIA
    • Reporter gene
    • cAMP
    • Toxicity
    • Screens
    • Kinetics

SoftMax® Pro Microplate Data Acquistion & Analysis Software, Molecular Devices industry leading all-in-one data acquisition and analysis software comes standard with the Paradigm Platform. SpectraDrop™ Plate protocols that enable Micro Volume applications measurement are also included.


  • The most extensive library of "Ready-to-Run" application protocols to get you started faster
  • Advanced calculations including non-linear fitting metrics for in-depth analysis
  • Syntax Helper to simplify complex calculations
  • Tools to customize reporting and exporting of data
  • GxP support for 21 CFR Part 11 compliance is available







General Specifications

Light source
High Powered LED or Flash Lamp
Plate formats
6- to 1536-well
Temperature range
4°C above ambient to 45°C
± 0.75°C
Linear, Orbital
Endpoint measurement
All Modes
Kinetic measurement
All Modes
Well area scanning
All Modes
Wavelength scanning
Dimensions W Ã-H Ã-D cm (in.)
39 (15.4) -45 (17.7) -63 (24.8)
Weight, without cartridges
45 kg (99 lbs)
Power source
100-240 VAC, 2 A, 50/60 Hz
Robotic compatible
Linear dynamic range
> 5 decades

Technical Specifications

Fluorometry (FI) (Fluorescein)
96-well (200 μL)
0.100 fmol
0.020 fmol
384-well (75 μL)
0.100 fmol
0.012 fmol
1536-well (8 μL)
0.030 fmol
0.003 fmol
Fluorescence Polarization (FP) (Fluorescein 1 nM, SD)
96-well (200 μL)
3.0 mP
0.5 mP
384-well (75 μL)
3.0 mP
0.5 mP
1536-well (8 μL)
6.0 mP
1.5 mP
Time Resolved Fluorescence (TRF) (Europium)
96-well (200 μL)
10.0 amol
2.5 amol
384-well (75 μL)
5.0 amol
1.0 amol (100 μL)
1536-well (8 μL)
1.5 amol
0.6 amol
Luminescence (Lum) (ATP-Glow)
96-well (200 μL)
0.50 fmol
0.30 fmol
384-well (50 μL)
0.50 fmol
0.30 fmol
1536-well (8 μL)
0.20 fmol
0.15 fmol
 * For properly functioning, operated and maintained equipment.
Wavelength Range
230-1000 nm
Wavelength Selection
Monochromator (1.0 nm increments)
Wavelength Bandwidth
4.0 nm
Wavelength Accuracy
± 1 nm
Wavelength Repeatability
± 0.5 nm
Photometric Range
3.5 OD
Photometric Resolution
0.0001 OD
Photometric Accuracy
± (2% + 0.010 OD), 0-2 OD @ 405 nm
Photometric Precision
± (1% + 0.005 OD), 0-2 OD @ 405 nm

Related Videos

This video features the SpectraMax Paradigm Multi-Mode Microplate Reader with the TUNE Cartridge.






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SpectraMax® Paradigm Multi-Mode Microplate Reader


Part Number

SpectraMax Paradigm Multi-Mode Microplate Reader with SoftMax Pro Software PARADIGM
SpectraMax Absorbance (ABS) Detection Cartridge
SpectraMax Tunable Wavelength (TUNE) Detection Cartridge
SpectraMax Multi-mode (MULTI) Detection Cartridge
SpectraMax Fluorescence Intensity (FI) (coum-fluor) Detection Cartridge
SpectraMax Fluorescence Intensity (FI) (fluor-rhod) Detection Cartridge
SpectraMax Fluorescence Intensity (FI) (Cy3-Cy5) Detection Cartridge
SpectraMax Fluorescence Intensity (FI) (CFP-YFP) Detection Cartridge
SpectraMax Fluorescence Intensity (FI) GeneBLAzer Detection Cartridge
SpectraMax Fluorescence Intensity Dual Label (FI-DL) (MultiTox-Fluor) Detection Cartridge
SpectraMax Time Resolved Fluorescence (TRF) Detection Cartridge
SpectraMax Fluorescence Polarization (FP) (fluorescein) Detection Cartridge
SpectraMax Fluorescence Polarization (FP) (rhodamine) Detection Cartridge
SpectraMax Cisbio HTRF Detection Cartridge
SpectraMax Glow Luminescence (LUM) Detection Cartridge
SpectraMax Dual Color Luminescence (LUM) (Chroma-Glo) Detection Cartridge
SpectraMax  Glow Luminescence (LUM) (96) Detection Cartridge
SpectraMax Glow Luminescence (LUM) (384) Detection Cartridge
SpectraMax Dual Color Luminescence (LUM) (BRET2TM) Detection Cartridge
SpectraMax AlphaScreen 384 STD Detection Cartridge
SpectraMax AlphaScreen 384 HTS Detection Cartridge
SpectraMax AlphaScreen 1536 HTS Detection Cartridge

Other Accessories

 Product Name
Part Number
SpectraDrop™ Micro-Volume Microplate Starter Kit
SpectraDrop™ Micro-Volume Microplate HTS Kit
Multi-Mode Validation Plate for SpectraMax® Paradigm® & FilterMax™ F3/F5 Systems