NEW App Notes: Detect cell viability and cell proliferation  Download now >>

WIN a one-year SoftMax Pro Import Feature subscription.  Enter contest >>

FilterMax F3 & F5 Multi-Mode Microplate Readers

See the future of your projects, sooner, with a beautifully designed F3 or F5 FilterMax Multi-Mode Microplate Reader

  • FilterMax F3 Multi-Mode Microplate Reader
    The FilterMax F3 offers a stunning set of features at an economical price. With the F3 Reader, you get visible-wavelength absorbance, top-reading fluorescence intensity, and glow luminescence modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements in 96- to 384-well plates. Easily accessible filter slides give you wavelength selection options from 340 nm to 650 nm.
  • FilterMax F5 Multi-Mode Microplate Reader
    The FilterMax F5 gives you an even richer set of features while maintaining an economical price-to-performance ratio. With the F5 Reader, you get UV and visible absorbance, top and bottom fluorescence intensity, glow luminescence, fluorescence polarization, and time-resolved fluorescence modes, plus temperature control and linear and orbital shaking for programmable endpoint, kinetic, multiple wavelength, linear scan, and area measurements in 6- to 1536-well plates. Filter slides on the F5 Reader give you wavelength selection options from 230 nm to 750 nm.

The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide value and flexibility for a variety of applications capable of performing:

  • Absorbance detection in the visible range (340 nm to 650 nm, F3) or UV and visible range (230nm to 650nm, F5)
  • Fluorescence Intensity (FI) top read
  • Fluorescence Intensity (FI) top read & bottom read (F5)
  • Luminescence (glow)
  • Fluorescence Polarization (FP) - F5
  • Time-Resolved Fluorescence (TRF) - F5
  • Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan
  • Linear and Orbital shaking
  • Temperature Control

The FilterMax Series can operate as standalone instruments or can be integrated seamlessly with laboratory automation platforms, and is ideal for a broad range of applications, including drug discovery, genomics, proteomics and cell-based research. The unique patented design ensures precise performance and sensitivity across all detection modes.

Benefits

  • Performance driven specifications for sensitivity, dynamic range, accuracy, precision and linearity
  • Affordable multi-mode microplate reader
  • Excellent price / performance ratio

Features

  • 3 or 5 Modes of detection
  • Automatic z-height optimization (top read) (F5 only)
  • Patented LED intensity adjustment
  • Up to 384-well format
  • Filter based multi-mode

From basic research to drug discovery, the FilterMax series cover a range of applications at an affordable price without sacrificing performance of your multi-mode microplate reader.

Representative Applications

  • Apoptosis
  • Cell proliferation, viability, cytotoxicity
  • ELISA
  • Endotoxin tests, agglutination assays
  • Enzyme activity and kinetic assays
  • Genotyping
  • GPCRs
  • Molecular interactions
  • Nucleic acid and protein quantitation
  • Proliferation assays
  • Reporter gene assays with fluorescent proteins (GFP)

SoftMax® Pro Software, Molecular Devices industry leading all-in-one data acquisition and analysis software, offers:

  • The most extensive library of "Ready-to-Run" application protocols to get you started faster
  • Advanced calculations including non-linear fitting metrics for in-depth analysis
  • Syntax Helper to simplify complex calculations
  • Tools to customize reporting and exporting of data

GxP support for 21 CFR Part 11 compliance is also available for Paradigm customers.

General Specifications

  FilterMax F3 FilterMax F5
Light Source
High Power LEDs
High Power LEDs + Deuterium Lamp
Detectors
Silicon Photodiode + PMT
Filters
1 EX + 1 EM Filter Slides (6 filter capacity)
 
2 EX + 1 EM Filter Slides (6 filter capacity)
Plate Formats
96-384 well
 
6-1536 well
Measurement Methods
Endpoint, Kinetic, Linear Scan, Area Scan
Shaking
Linear + Orbital (3 speeds)
Temperature Control
n/a
Ambient +4°C to 45°C
Power/Voltage
100-240VAC; 50/60Hz
Dimensions
39cm W x 24cm H x 58cm L
Weight
25 kg
Fluorescence Intensity (FI) - Top Read
Wavelength Range
 
340-650nm
 
230-750nm
Detection Limit--96 well plate
 
3 fmol/200µl fluorescein
 
0.5 fmol/200µl fluorescein
Detection Limit--384 well plate
 
3 fmol/100µl fluorescein
 
0.4 fmol/100µl fluorescein
Linear Dynamic Range
 
6 decades
Read Time (Fly mode)
 
18sec/96well; 37sec/384well
Fluorescence Intensity (FI) - Bottom Read
Wavelength Range
 
n/a
 
300-650nm
Detection Limit--96 well plate
 
n/a
 
25 fmol/200µl fluorescein
Detection Limit--384 well plate
 
n/a
 
15 fmol/100µl fluorescein
Time-Resolved Fluorescence (TRF)
Wavelength Range
 
n/a
 
340-750nm
Detection Limit--96 well plate
 
n/a
 
30 amol/200µl Europium
Detection Limit--384 well plate
 
n/a
 
15 amol/100µl Europium
Linear Dynamic Range
 
n/a
 
5 decades
Read Time (1sec integration)
 
n/a
 
180sec/96well; 690sec/384well
Fluorescence Polarization (FP)
Wavelength Range
 
n/a
 
300-750nm
Detection Limit--96 well plate
 
n/a
 
3 mP at 10nM Fluorescein
Detection Limit--384 well plate
 
n/a
 
4 mP at 10nM Fluorescein
Read Time (Fly mode)
 
n/a
 
38sec/96well; 79sec/384well
Luminescence (LUM) - Glow
Wavelength Range
 
400-650nm
 
400-750nm
Detection Limit--96 well plate
 
2 fmol ATP
Linear Dynamic Range
 
6 decades
Read Time (Fly mode)
 
18sec/96well; 37sec/384well
Absorbance (Abs)
Wavelength Range
 
340-650nm
 
230-650nm
Indication Range
 
0-3.5 OD
Accuracy
 
<+1%+10mOD at 2 OD
Reproducibility
 
<+0.5% and +0.005 OD at 2 OD
Read Time
 
18sec/96well; 37sec/384well
Operating System
Item
Minimum
Recommended
Processor Single-core, 2 GHz or faster Quad core or faster
Operating System
Window 7, 32-bit or 64-bit (x86 or x64) and .NET Framework 4.0 (.NET Framework is installed automatically by the SoftMax Pro Software installer if necessary)
Windows 7, 32-bit or 64-bit (x86 or x64), or Windows 8, 32-bit or 64-bit (x86 or x64)
Data Connection RS-232 serial port or USB 2.0 port (depending on the instrument) USB 2.0 ports; For those that require an RS-232 serial port, you can use a USB 2.0 port with a Keyspan, USB-to-serial adapter. Keyspan USB-to-serial adapters have been field tested and approved by Molecular Devices.
Memory 2 GB RAM 4GB RAM; If running on a virtual machine, Molecular Devices recommends a minimum of 6 GB RAM. For automation, Molecular Devices recommends a minimum of 8 GB RAM.
Hard Disk 500 MB of available space 1 GB of available space or more
Graphics Display Graphics display adapter 1024 x 768 or higher resolution display 32-bit graphics display with 265 MB video RAM;
1280 x 1024 or higher resolution display
Software Installation and Activation CD/DVD drive; Internet connection or external USB drive CD/DVD drive; Internet connection

FilterMax F3 & F5 Multi-Mode Microplate Readers

Product

Product Number

FilterMax F3 Microplate Reader F3
FilterMax F5 Microplate Reader F5
FilterMax FLUORESCENCE EXCITATION FILTER 360NM BW 35NM
6590-0042
FilterMax FLUORESCENCE EXCITATION FILTER 485NM BW 20NM
6590-0043
FilterMax FLUORESCENCE EXCITATION FILTER 535NM BW 25NM
6590-0044
FilterMax FLUORESCENCE EXCITATION FILTER 430NM BW 35NM
6590-0045
FilterMax FLUORESCENCE EXCITATION FILTER 590NM BW 20NM
6590-0046
FilterMax FLUORESCENCE EXCITATION FILTER 320NM BW 25NM
6590-0047
FilterMax FLUORESCENCE EXCITATION FILTER 405 NM BW 30NM
6590-0048
FilterMax FLUORESCENCE EXCITATION FILTER 465NM BW 35NM
6590-0049
FilterMax FLUORESCENCE EXCITATION FILTER 500NM BW 25NM
6590-0050
FilterMax FLUORESCENCE EXCITATION FILTER 518NM BW 26NM
6590-0051
FilterMax FLUORESCENCE EXCITATION FILTER 525 NM BW 45NM
6590-0052
FilterMax FLUORESCENCE EXCITATION FILTER 540NM BW 25NM
6590-0053
FilterMax FLUORESCENCE EXCITATION FILTER 550NM BW 10 NM
6590-0054
FilterMax FLUORESCENCE EXCITATION FILTER 585NM BW 10NM                                               
6590-0055
Fluorescence (FI) Emission Filters
Description
 
FilterMax FLUORESCENCE EMISSION FILTER 465NM BW 35NM
6590-0056
FilterMax FLUORESCENCE EMISSION FILTER 535NM BW 25NM
6590-0057
FilterMax FLUORESCENCE EMISSION FILTER 595NM BW 35NM
6590-0058
FilterMax FLUORESCENCE EMISSION FILTER 625NM BW 35NM
6590-0059
FilterMax FLUORESCENCE EMISSION FILTER 430NM BW 35NM
6590-0060
FilterMax FLUORESCENCE EMISSION FILTER 650NM BW 40NM
6590-0061
FilterMax FLUORESCENCE EMISSION FILTER 485NM BW 20NM
6590-0062
FilterMax FLUORESCENCE EMISSION FILTER 500 NM BW 25NM
6590-0063
FilterMax FLUORESCENCE EMISSION FILTER 550NM BW 10NM
6590-0064
FilterMax FLUORESCENCE EMISSION FILTER 574 NM BW 25NM
6590-0065
FilterMax FLUORESCENCE EMISSION FILTER 585NM BW 10NM
6590-0066
FilterMax FLUORESCENCE EMISSION FILTER 635NM BW 35NM
6590-0067
FilterMax FLUORESCENCE EMISSION FILTER 660NM BW 32NM
6590-0068
FilterMax FLUORESCENCE EMISSION FILTER 668NM BW 12NM
6590-0069
FilterMax FLUORESCENCE EMISSION FILTER 670NM BW 40NM
6590-0070
 
Fluorescence Polarization (FP) Filters
Product Name
Product Number
FilterMax FLUORESCENCE POLARIZATION (FP) Excitation Filter Visible                               6590-0071
FilterMax FLUORESCENCE POLARIZATION (FP) Emission Filter Visible (QTY 2)
6590-0072
 Time-Resolved Fluorescence (TRF) Filters
Product Name
Product Number
FilterMax TIME RESOLVED FLUORESCENCE (TRF) Excitation Filter 370NM BW80 NM                         
6590-0073
 Absorbance Filters
Product Name
Product Number
FilterMax ABSORBANCE FILETER 280NM BW 5,5NM                                                                     6590-002
FilterMax ABSORBANCE FILTER 260NM BW 5,5NM 6590-001
FilterMax ABSORBANCE FILTER 320NM BW 5,5NM
6590-0003
FilterMax ABSORBANCE FILTER 420NM BW 8NM
6590-0004
FilterMax ABSORBANCE FILTER 450NM BW 8NM
6590-0005
FilterMax ABSORBANCE FILTER 465NM BW 8NM
6590-0006
FilterMax ABSORBANCE FILTER 470NM BW 8NM
6590-0007
FilterMax ABSORBANCE FILTER 480NM BW 8NM
6590-0008
FilterMax ABSORBANCE FILTER 490NM BW 8NM
6590-0009
FilterMax ABSORBANCE FILTER 492NM BW 8NM
6590-0010
FilterMax ABSORBANCE FILTER 510NM BW 8NM
6590-0011
 FilterMax ABSORBANCE FILTER 515NM BW 8NM
6590-0012
 FilterMax ABSORBANCE FILTER 520NM BW 8NM
6590-0013
FilterMax ABSORBANCE FILTER 532NM BW 8NM
  6590-0014
FilterMax ABSORBANCE FILTER 540NM BW 8NM
6590-0015
FilterMax ABSORBANCE FILTER 548NM BW 8NM
6590-0016
 FilterMax ABSORBANCE FILTER 550NM BW 8NM
6590-0017
FilterMax ABSORBANCE FILTER 560NM BW 8NM
6590-0018
 FilterMax ABSORBANCE FILTER 562NM BW 8NM
6590-0019
FilterMax ABSORBANCE FILTER 570NM BW 8NM
6590-0020
 FilterMax ABSORBANCE FILTER 575NM BW 8NM
 6590-0021
FilterMax ABSORBANCE FILTER 580NM BW 8NM
6590-0022
FilterMax ABSORBANCE FILTER 590NM BW 8NM
6590-0023
 FilterMax ABSORBANCE FILTER 595NM BW 8NM
6590-0024
 FilterMax ABSORBANCE FILTER 600NM BW 8NM
6590-0025
FilterMax ABSORBANCE FILTER 610NM BW 8NM
6590-0026
FilterMax ABSORBANCE FILTER 620NM BW 8NM
6590-0027
FilterMax ABSORBANCE FILTER 630NM BW 8NM
6590-0028
 FilterMax ABSORBANCE FILTER 640NM BW 8NM
6590-0029
 FilterMax ABSORBANCE FILTER 645NM BW 8NM
6590-0030
FilterMax ABSORBANCE FILTER 650NM BW 8NM
6590-0031
FilterMax ABSORBANCE FILTER 340NM BW 10NM
 6590-0032
 FilterMax ABSORBANCE FILTER 350NM BW 10NM
 6590-0033
FilterMax ABSORBANCE FILTER 360NM BW 10NM
6590-0034
 FilterMax ABSORBANCE FILTER 370NM BW 10NM
6590-0035
FilterMax ABSORBANCE FILTER 380NM BW 10NM
6590-0036
FilterMax ABSORBANCE FILTER 405NM BW 10NM
6590-0037
 FilterMax ABSORBANCE FILTER 414NM BW 10NM
6590-0038
 FilterMax ABSORBANCE FILTER 260NM BW 12NM
6590-0039
FilterMax ABSORBANCE FILTER 280NM BW 12NM
6590-0040
FilterMax ABSORBANCE FILTER 320NM BW 12NM
 6590-0041

Number of Citations*: 126

Latest Citations:
For a complete list, please click here.

Identification of phenolics in litchi and evaluation of anticancer cell proliferation activity and intracellular antioxidant activity

L Wen, L You, X Yang, J Yang, F Chen, Y Jiang… - Free Radical Biology …, 2015 - Elsevier
... Quercetin was used as positive control. The fluorescence generation was measured every 5
min for 35 cycles using a multimode microplate reader (Filter Max F5, Molecular Devices, USA)
with an excitation wavelength of 485 nm and an emission wavelength of 538 nm. ...

The NADPH oxidase inhibitor DPI can abolish hypoxia-induced apoptosis of human kidney proximal tubular epithelial cells through Bcl2 up-regulation via ERK …

H Song, IY Han, Y Kim, YH Kim, IW Choi, SK Seo… - Life sciences, 2015 - Elsevier
... The cells were then incubated with 10 μL of WST-1 reagent at 37 °C for 4 h. The absorbance
of the cell cultures was measured at a wavelength of 450 nm using a Molecular Devices Filter
MAX F5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). ...
 

[HTML] Rapid and Liquid-Based Selection of Genetic Switches Using Nucleoside Kinase Fused with Aminoglycoside Phosphotransferase

M Tominaga, K Ike, S Kawai-Noma, K Saito, D Umeno - PloS one, 2015 - dx.plos.org
... The fluorescence intensity (488 nm excitation, 507 nm emission [16]) of each sample was
measured using a fluorescence microtiter-plate reader (FilterMax F5, Molecular Devices,
Sunnyvale, CA, USA) with excitation at 485 nm and emission at 535 nm. ...

Phenolic contents and cellular antioxidant activity of Chinese hawthorn “Crataegus pinnatifida”

L Wen, X Guo, RH Liu, L You, AM Abbasi, X Fu - Food chemistry, 2015 - Elsevier
... as a blank. The fluorescence generation was measured every 5 min for 35 cycles
using a multi-mode microplate reader (Filter Max F5, Molecular Devices, USA) with
485 nm excitation and 538 nm emission. The ORAC values ...
 

[PDF] Role of 2, 3-cis Structure of (-)-Epicatechin-3, 5-O-digallate in Inhibition of HeLa S3 Cell Proliferation

K Mori, Y Ayano, Y Hamada, T Hojima… - Nat Prod Chem …, 2015 - esciencecentral.org
... Viable cells were then assessed using a microplate reader (Filter Max F5 multi-mode
microplate reader; Molecular Devices; Downingtown; PA; USA) to measure the OD at 570
nm. Detection of non-specific binding between polyphenols and BSA ...
 

Involvement of reactive oxygen species in the cytotoxic effect of acid-electrolyzed water

T Mokudai, T Kanno, Y Niwano - The Journal of toxicological sciences, 2015 - jlc.jst.go.jp
... for a further 24 hr before determining cell viability by the methyl thia- zolyl tetrazolium (MTT) assay,
in which an insoluble for- mazan converted from MTT was colorimetrically quanti- fied at 595
nm using a microplate reader (FilterMax F5; Molecular Devices, Sunnyvale, CA, USA ...

Changes in the concentrations of four maternal steroids during embryonic development in the threespined stickleback (Gasterosteus aculeatus)

RT Paitz, BC Mommer, E Suhr… - Journal of Experimental …, 2015 - Wiley Online Library
... Following the manufacturer's protocol, 96-well ELISA plates were prepared and absorbance
read at 405 nm for each sample (Enzo Life Sciences) on a FilterMax F3 microplate reader
(Molecular Devices, Sunnyvale, CA, USA) and absorbance data averaged across duplicate ...

Effects of Hyperbaric Oxygen and Preconditioning on Wound Healing in Colonic Anastomoses

Y Poyrazoglu, T Topal, R Yuksel… - Journal of …, 2015 - informahealthcare.com
... MDA) at 535 nm. Absorption was measured with a spectrophotometer (Filter Max
F5 Multimode Microplate reader, Molecular Devices, Austria). MDA levels, expressed
as mmol/gprotein, are an indicator of lipid peroxidation. ...
 

Postantifungal-like effect of sublethal treatment of Candida albicans with acid-electrolyzed water

T Mokudai, T Kanno, Y Niwano - Archives of oral biology, 2015 - Elsevier
... plate. Immediately after the addition of the sample, the plate was placed in a
microplate reader (FilterMax F5, Molecular Devices, Sunnyvale, CA, USA) with the
temperature control at 37 °C, and the OD595 was recorded. The ...
 

Draft genome sequences of Klebsiella pneumoniae clinical type strain ATCC 13883 and three multidrug-resistant clinical isolates

BA Arivett, DC Ream, SE Fiester, K Mende… - Genome …, 2015 - Am Soc Microbiol
... determined by the SYBR green (Life Technologies, Grand Island, NY) standard curve method
in a black 96-well plate (Corning, Tewksbury, MA, USA) using a FilterMax F5 spectrophotometer
with Multimode Analysis software version 3.4.0.25 (Molecular Devices, Sunnyvale, CA ...

 

 

* as of June 26, 2015. Source: Google Scholar.