Comparison of Microplate Readers with Luminescence Detection

SpectraMax i3 Multi-Mode Detection PlatformSpectraMax Paradigm Multi-Mode Microplate ReaderSpectraMax M Series Multi-Mode Microplate ReadersFlexStation 3 Multi-Mode Microplate ReaderSpectraMax L Microplate Reader
DetectorPhotomultiplier tubes (PMT) and/or photodiode2 PMTPhoto-multiplier tube2 PMT1, 2, or 6 Ultra-fast Photomultiplier Tubes
Spectral range300-850 nm300-850 nm250-850 nm250-850 nm380-630 nm
Dynamic range> 6 decades> 6 decades> 6 decades> 6 decades> 9 decades
Sensitivity15 pM for 96-wells, 30 pM for 384-wells (Optimized for ATP-Glow)1.25 pM for 96-wells, 3 pM for 384-wells, 19 pM for 1536-wells (Optimized for ATP-Glow)43 pM ATP in 96 wells (optimized)<2 fg/well for firefly luciferase in 96- and 384-well top read< 20 attomol ATP per well
Microplate format6 to 1536 wells*6- to 1536-wells6-384-well microplates6-384 (96- and 384-wells with pipetting)96- and 384-well microplates
Cross-talk< 0.3% in white 96- and 384-well microplates<0.3% in white 96- and 384-well microplates<0.3% in white 96- and 384-well microplates< 3 x 10-5 (96-well)
Read modesSpectral, Endpoint, KineticEndpoint, KineticEndpoint, KineticEndpoint, Kinetic, Fast KineticEndpoint, Kinetic, Fast Kinetic, Dual-read
Read times0:30 minutes for 96-wells, 1:15 minutes for 384-wells2:00 minutes for 96-wells, 7:00 minutes for 384-wells2:00 minutes for 96-wells, 7:00 minutes for 384-wells0.1 up to 3600 sec. in steps of 0.1 sec.
Injectors/pipettorsOption availableOption available8- or 16-channel pipettor2 variable volume injectors
Injection/pipettor volumesMax. volume 200uL for 8-channel and 30 uL for 16-channel
Temperature control4 degrees above ambient to 45 degrees C2 degrees C above ambient to 45 degrees C2 degrees C above ambient to 60 degrees C2 degrees C above ambient to 45 degrees C5 degrees C above ambient to 45 degrees C
Software SoftMax Pro SoftwareSoftMax Pro SoftwareSoftMax Pro SoftwareSoftMax Pro SoftwareSoftMax Pro Software
Validation & compliance toolsIQ/OQ Protocols, SoftMax Pro GxP Compliance Software, SpectraTest LM1 Validation PlateSoftMax Pro GxP Compliance SoftwareIQ/OQ Protocols, FDA 21 CFR Part 11 Compliance, SpectraTest LM1 Validation PlateIQ/OQ/PQ Protocols, FDA 21 CFR Part 11 ComplianceSoftware Validation Package, IQ/OQ Documentation, Physical Validation Test Plate
Robotic compatible

* 1536 detection available via detection cartridges