SpectraMax Plus 384 Microplate Reader

UV-visible absorbance microplate reader, providing ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates

The SpectraMax® Plus 384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. For more sample throughput, the system can be easily integrated into a full robotic system and easily adapts to fit sample processing requirements. The SpectraMax Plus384 reader has a wide wavelength range from 190 to 1000 nm and supports an expanded range of applications including DNA/RNA quantification, enzyme kinetic assays, and ELISA based assays.


  • No filter system: Includes monochromator allowing up to 6 wavelengths at a time in the visible wavelength range and scanning ranges from 190-1000 nm in 1 nm increments
  • Easy assay optimization: Read a variety of sample formats from cuvettes to 96- and 384-well microplates in endpoint, kinetic, and spectral scan modes
  • Correction for varying microplate well volumes and the elimination of standard curves based on PathCheck® Technology, the only temperature-independent pathlength correction method
  • Better experimental signal discrimination using narrow absorbance bandpass for superior peak resolution and increased accuracy.
  • Monitoring of temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
  • Thorough mixing of samples in microplate wells with user-programmable Automix feature
  • Comprehensive data analysis with SoftMax® Pro Software
  • Seamless integration with the StakMax® Microplate Handling Stacker


Microplate Reader Validation

The SpectraMax Plus384 Microplate Reader supports a multitude of absorbance assay categories including:

  • Cell viability, proliferation, and cytotoxicity
  • ELISAs and immunoassays
  • Endotoxin detection
  • Enzyme assays
  • Micro volume applications
  • Microbial growth
  • Nucleic acid (DNA) quantitation
  • Phosphotases/kinases
  • Protein quantitation
  • Reporter gene assays

SoftMax Pro Software, Molecular Devices industry leading all-in-one data acquisition and analysis software, offers:

  • The most extensive library of supported application protocols to get you started faster
  • Optional tools for regulated labs with SoftMax Pro GxP Compliance Software
  • Advanced calculations including non-linear fitting metrics for in-depth analysis
  • The only Mac- and Windows-native application support for seamless exchange of data files between operating systems
  • Proven integrations with leading automation and LIMS platforms for partnered solutions

General Specifications

Dimensions (in) 8.6 H x 22.8 W x 15 D
Dimensions (cm) 22 H x 58 W x 38 D
Weight 30 lbs (13.6 kg)
Power Source 90-250 VAC, 50/60 Hz

Performance Specifications

Wavelength Range 190-1000nm
Wavelength Selection Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth 2 nm
Wavelength Accuracy < ± 1.0 nm
Wavelength Repeatability ± 0.2 nm
Photometric Range 0.0 to 4.0 nm
Photometric Resolution 0.001 OD
Photometric Accuracy (Microplate) < ± 0.006 PD ± 1.0%, 0.0 - 2.0 OD
Photometric Accuracy (Cuvette) < ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Baseline Flatness < 0.001 OD
Stray Light < 0.05% @ 230 nm

Microplate Read Time

96 wells 9 seconds
384 wells 29 seconds

Speed Read Mode

96 wells 5 seconds
384 wells 16 seconds
Temperature Range Ambient + 4 °C up to 45 °C
Temperature Uniformity (Microplate) ± 0.5 °C @ 37 °C

SpectraMax® Plus 384 Microplate Reader

Product Part Number
SpectraMax Plus 384 Microplate Reader PLUS 384
SpectraTest ABS1 Absorbance Validation Plate 0200-6117
StakMax Microplate Handling Stacker StakMax
Quartz 96-well microplate for UV measurements R8024
Quartz 96-well half-area microplate for UV measurements R8028
SpectraDrop™ Micro-Volume Microplate Starter Kit 0200-6262
SpectraDrop™ Micro-Volume Microplate HTS Kit 0200-6267
Cuvette Validation Set 0200-2420
Microplate Reader Shelf 9000-0756