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SpectraMax Plus 384 Microplate Reader

Begin your brilliant projects quickly, and then go even faster, with rapid UV-Vis absorbance reads for a variety of sample formats

Get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. The SpectraMax® Plus 384 Microplate Reader gives you walk-up convenience for both standard spectrophotometer and microplate reader applications on the same instrument. Monochromator wavelength selection saves you the time and trouble of ever having to change out filters. Typical applications for this powerful reader include DNA and RNA quantification, enzyme kinetic assays, and ELISA based assays. When your project is ready for high throughput, take advantage of StakMax® automated microplate handling compatibility for full robotic integration.

Benefits

  • No filter system: Includes monochromator allowing up to 6 wavelengths at a time in the visible wavelength range and scanning ranges from 190-1000 nm in 1 nm increments
  • Easy assay optimization: Read a variety of sample formats from cuvettes to 96- and 384-well microplates in endpoint, kinetic, and spectral scan modes
  • Correction for varying microplate well volumes and the elimination of standard curves based on PathCheck® Technology, the only temperature-independent pathlength correction method
  • Better experimental signal discrimination using narrow absorbance bandpass for superior peak resolution and increased accuracy.
  • Monitoring of temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
  • Thorough mixing of samples in microplate wells with user-programmable Automix feature
  • Comprehensive data analysis with SoftMax® Pro Software
  • Seamless integration with the StakMax® Microplate Handling Stacker

 

Microplate Reader Validation

The SpectraMax Plus384 Microplate Reader supports a multitude of absorbance assay categories including:

  • Cell viability, proliferation, and cytotoxicity
  • ELISAs and immunoassays
  • Endotoxin detection
  • Enzyme assays
  • Micro volume applications
  • Microbial growth
  • Nucleic acid (DNA) quantitation
  • Phosphotases/kinases
  • Protein quantitation
  • Reporter gene assays

SoftMax Pro Software, Molecular Devices industry leading all-in-one data acquisition and analysis software, offers:

  • The most extensive library of supported application protocols to get you started faster
  • Optional tools for regulated labs with SoftMax Pro GxP Compliance Software
  • Advanced calculations including non-linear fitting metrics for in-depth analysis
  • The only Mac- and Windows-native application support for seamless exchange of data files between operating systems
  • Proven integrations with leading automation and LIMS platforms for partnered solutions

General Specifications

Dimensions (in) 8.6 H x 22.8 W x 15 D
Dimensions (cm) 22 H x 58 W x 38 D
Weight 30 lbs (13.6 kg)
Power Source 90-250 VAC, 50/60 Hz

Performance Specifications

Wavelength Range 190-1000nm
Wavelength Selection Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth 2 nm
Wavelength Accuracy < ± 1.0 nm
Wavelength Repeatability ± 0.2 nm
Photometric Range 0.0 to 4.0 nm
Photometric Resolution 0.001 OD
Photometric Accuracy (Microplate) < ± 0.006 PD ± 1.0%, 0.0 - 2.0 OD
Photometric Accuracy (Cuvette) < ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Baseline Flatness < 0.001 OD
Stray Light < 0.05% @ 230 nm

Microplate Read Time

96 wells 9 seconds
384 wells 29 seconds

Speed Read Mode

96 wells 5 seconds
384 wells 16 seconds
Temperature Range Ambient + 4 °C up to 45 °C
Temperature Uniformity (Microplate) ± 0.5 °C @ 37 °C

SpectraMax® Plus 384 Microplate Reader

Product Part Number
SpectraMax Plus 384 Microplate Reader PLUS 384
SpectraTest ABS1 Absorbance Validation Plate 0200-6117
StakMax Microplate Handling Stacker StakMax
Quartz 96-well microplate for UV measurements R8024
Quartz 96-well half-area microplate for UV measurements R8028
SpectraDrop™ Micro-Volume Microplate Starter Kit 0200-6262
SpectraDrop™ Micro-Volume Microplate HTS Kit 0200-6267
Cuvette Validation Set 0200-2420
Microplate Reader Shelf 9000-0756