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SpectraMax Plus 384 Microplate Reader

Begin your brilliant projects quickly, and then go even faster, with rapid UV-Vis absorbance reads for a variety of sample formats

Get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. The SpectraMax® Plus 384 Microplate Reader gives you walk-up convenience for both standard spectrophotometer and microplate reader applications on the same instrument. Monochromator wavelength selection saves you the time and trouble of ever having to change out filters. Typical applications for this powerful reader include DNA and RNA quantification, enzyme kinetic assays, and ELISA based assays. When your project is ready for high throughput, take advantage of StakMax® automated microplate handling compatibility for full robotic integration.


  • No filter system: Includes monochromator allowing up to 6 wavelengths at a time in the visible wavelength range and scanning ranges from 190-1000 nm in 1 nm increments
  • Easy assay optimization: Read a variety of sample formats from cuvettes to 96- and 384-well microplates in endpoint, kinetic, and spectral scan modes
  • Correction for varying microplate well volumes and the elimination of standard curves based on PathCheck® Technology, the only temperature-independent pathlength correction method
  • Better experimental signal discrimination using narrow absorbance bandpass for superior peak resolution and increased accuracy.
  • Monitoring of temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
  • Thorough mixing of samples in microplate wells with user-programmable Automix feature
  • Quickly quantify DNA, RNA, and protein samples as low as 2 µL with the SpectraDrop Micro-Volume Microplate
  • Comprehensive data analysis with SoftMax® Pro Software
  • Seamless integration with the StakMax® Microplate Handling Stacker


Microplate Reader Validation

The SpectraMax Plus384 Microplate Reader supports a multitude of absorbance assay categories including:

  • Cell viability, proliferation, and cytotoxicity
  • ELISAs and immunoassays
  • Endotoxin detection
  • Enzyme assays
  • Micro volume applications
  • Microbial growth
  • Nucleic acid (DNA) quantitation
  • Phosphotases/kinases
  • Protein quantitation
  • Reporter gene assays


SoftMax Pro Software simplifies setting up your assays by providing:

  • Simple set-up and automatic instrument recognition
  • Pre-defined protocols for endpoint and kinetic ELISA protein plus quantification assays such as Bradford, Lowry, BCA, and DC protein assays
  • A wide variety of scan types: Endpoint, Kinetic, Spectrum* and Well Scanning*
  • Standard data reduction settings are preselected as default
  • Automatic data recovery feature


SoftMax Pro Software offers a variety of customizing options:

  • Predefined calculation options for common data analysis functions    
  • Automatic export to Microsoft Excel
  • Instrument settings and plate read area can be adjusted to suit your experiment's needs
  • Custom data reduction settings can be selected for optimal signal selection
  • Full reports, with font flexibility, and live mini-tables and graphs are now available within the Notes section to simplify the result sharing experience      
  • Contemporary user community enabling protocol exchange and expertise
    sharing among our numerous users

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* available in select instrument models

General Specifications

Dimensions (in) 8.6 H x 22.8 W x 15 D
Dimensions (cm) 22 H x 58 W x 38 D
Weight 30 lbs (13.6 kg)
Power Source 90-250 VAC, 50/60 Hz

Performance Specifications

Wavelength Range 190-1000nm
Wavelength Selection Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth ≤ 2.0 nm
Wavelength Accuracy < ± 1.0 nm
Wavelength Repeatability ± 0.2 nm
Photometric Range 0.0 to 4.0 nm
Photometric Resolution 0.001 OD
Photometric Accuracy (Microplate) < ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Accuracy (Cuvette) < ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Precision ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
Stray Light < 0.05% @ 230 nm
Light Source Xenon Flash Lamp
Microplate Read Time - Endpoint 96-Well: 9 seconds
384-Well: 29 Seconds
Microplate Read Time - Kinetic 96-Well: 9 second interval
384-Well: 29 second internal
Microplate Read Time - Speed Read Mode 96-Well: 5 seconds
384-Well: 16 seconds
Temperature Range Ambient + 4 °C up to 45 °C
Temperature Uniformity (Microplate) ± 0.5 °C @ 37 °C

SpectraMax® Plus 384 Microplate Reader

Product Part Number
SpectraMax Plus 384 Microplate Reader PLUS 384
SpectraTest ABS1 Absorbance Validation Plate 0200-6117
StakMax Microplate Handling Stacker StakMax
Quartz 96-well microplate for UV measurements R8024
Quartz 96-well half-area microplate for UV measurements R8028
SpectraDrop™ Micro-Volume Microplate Starter Kit 0200-6262
SpectraDrop™ Micro-Volume Microplate HTS Kit 0200-6267
Cuvette Validation Set 0200-2420
Microplate Reader Shelf 9000-0756

Number of Citations*: 6,710

Latest Citations:
For a complete list, please click here.


Enhanced Amyloidogenic Processing of Amyloid Precursor Protein and Cell Death Under Prolonged Endoplasmic Reticulum Stress in Brain Endothelial Cells

AI Plácido, CR Oliveira, PI MoreiraCMF Pereira - Molecular neurobiology, 2015 - Springer
... The blue formazan crystals formed were dissolved in an equal volume of 0.04 M HCl in
isopropanol and quantified spectrophotometrically by measuring the absorbance at 570 nm using
a microplater reader (SpectraMax Plus 384, Molecular Devices, San Francisco, CA, USA). ...

Podocalyxin promotes cisplatin chemoresistance in osteosarcoma cells through phosphatidylinositide 3‑kinase signaling

Z Huang, Y Huang, H He, J Ni - Molecular medicine …, 2015 -
... described (18). The 96‑well plate used was purchased from Thermo Fisher Scientific
(Beijing, China) and the microplate reader (SpectraMax Plus 384) was purchased
from Molecular Devices, LLC (Beijing, China). The half ...

Elicitor activity of algino-oligosaccharide and its potential application in protection of rice plant (Oryza saliva L.) against Magnaporthe grisea

S Zhang, W Tang, L Jiang, Y Hou, F Yang… - Biotechnology & …, 2015 - Taylor & Francis
... After dilution of the reaction mixture with 3 mL of distilled water and centrifugation, the formation
of cinnamate was determined by measuring the absorbance increase at 290 nm with a SpectraMax
Plus 384 Microplate Reader (Molecular Devices, USA) against a corresponding ...

[HTML] Creatine protects against mitochondrial dysfunction associated with HIV-1 Tat-induced neuronal injury

PR Stevens, JW GawrylukL Hui, X Chen… - Current HIV …, 2015 -
... Absorbance was measured at 570 nm using a SpectraMax Plus 384 plate-reader (Molecular
Devices). ... Cells were washed once with 50 ml PBS and fluorescence was determined using
SpectraMax Plus 384 plate-reader (Molecular Devices). ...

Advancing Rhodobacter sphaeroides as a platform for expression of functional membrane proteins

M Erbakan, BS Curtis, BT Nixon, M Kumar… - Protein expression and …, 2015 - Elsevier
... To maintain consistency across batches with different levels of pigmentation, the optical density
was measured via Spectramax Plus 384 spectrophotometer (Molecular Devices, Sunnyvale
CA) at 660 nm to avoid interference by photosynthetic pigments [26]. ...

New serine-derived gemini surfactants as gene delivery systems

AM Cardoso, CM Morais, AR Cruz, SG Silva… - European Journal of …, 2015 - Elsevier
... The absorbance at 570 and 600 nm (information provided by the supplier) was measured
in a SPECTRAmax PLUS 384 spectrophotometer (Molecular Devices, Union City, CA)
and cell viability was calculated according to the equation: ...

Short-term effects of ultrahigh concentration cationic silica nanoparticles on cell internalization, cytotoxicity, and cell integrity with human breast cancer cell line (MCF-7 …

JH Seog, B Kong, D Kim, LM Graham, JS Choi… - Journal of Nanoparticle …, 2015 - Springer
... The plate was incubated in a humidified atmosphere containing 5 % CO2 at 37 °C for 2
h. The absorbance of each well was measured at 450 nm using a SpectraMax Plus 384
multiwell spectrophotometer (Molecular devices, Sunnyvale, USA). ...

Physicochemical and morphological analysis of ten tomato varieties identifies quality traits more readily manipulated through breeding and traditional selection …

AP Breksa, LD Robertson, JA Labate, BA King… - Journal of Food …, 2015 - Elsevier
... tomato samples. Assays were performed on the acetone:water (80:20) extracts and
absorbance measurements were made using a SpectraMax Plus 384
spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). For both ...

[HTML] Optimizing stem cell functions and antibacterial properties of TiO2 nanotubes incorporated with ZnO nanoparticles: experiments and modeling

W Liu, P Su, SC Arthur Gonzales III… - International journal …, 2015 -
... The SDS solution with the collected proteins was assessed for protein content by a Micro BCA
Protein Assay Kit (Pierce, Rockford, USA), and the protein concentrations were quantified using
a microplate spectrophotometer (SpectraMax Plus 384) at 562 nm. ...

Effect of Deployment Time on Endotoxin and Allergen Exposure Assessment Using Electrostatic Dust Collectors

B Kilburg-Basnyat, N Metwali, PS Thorne - Annals of Occupational Hygiene, 2015 - BOHS
... The working reagents from the kit (QuantiPro™ BCA Assay kit, Sigma-Aldrich) were mixed
according to the manufacturer's instructions and 150 µl were added to the samples and incubated
for 16h before measurement at 562nm (SpectraMax Plus 384, Molecular Devices, Inc.). ...



* as of June 26, 2015. Source: Google Scholar.