SpectraMax 340PC384 Microplate Reader

Versatile visible absorbance reader for 96- and 384- well microplate formats

The SpectraMax® 340PC384 is a tunable microplate reader with wavelegths from 340-850 nm in 1nm increments. Data can be aquired in endpoint, kinetic, and spectral scan modes and includes temperature control up to 45ºC, a robotics-compatible interface and SoftMax® Pro Data Analysis Software.  It includes the patented PathCheck® Sensor which measures the depth of the liquid in each microplate well. This feature can also be used to calculate concentrations without a standard curve and expand the dynamic range to 6+ OD. The SpectraMax 340PC384 supports a wide range of applications including ELISA, cytoxicity, ß- galatosidase reporter gene assays, and NAD(P)H-based assays.


  • No filter system: Includes monochromator allowing up to 6 wavelengths at a time in the visible wavelength range and scanning ranges from 340-850 nm in 1 nm increments
  • Easy assay optimization: Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine wavelength peaks
  • Correction for varying microplate well volumes and the elimination of standard curves based on PathCheck Technology, the only temperature-independent pathlength correction method
  • Thorough mixing of samples in microplate wells with user-programmable Automix feature
  • Comprehensive data analysis with SoftMax® Pro Software
  • Seamless integration with the StakMax® Microplate Handling Stacker

Microplate Reader Validation

The SpectraMax 340PC384 Microplate Reader supports a multitude of absorbance assay categories including:

  • Cell viability, proliferation, and cytotoxicity
  • ELISAs and immunoassays
  • Endotoxin detection
  • Enzyme assays
  • Microbial growth
  • Phosphotases/kinases
  • Protein quantitation
  • Reporter gene assays

SoftMax® Pro Software, Molecular Devices industry leading all-in-one data acquisition and analysis software, offers:

  • The most extensive library of supported application protocols to get you started faster
  • Optional tools for regulated labs with SoftMax Pro GxP Compliance Software
  • Advanced calculations including non-linear fitting metrics for in-depth analysis
  • The only Mac- and Windows-native application support for seamless exchange of data files between operating systems
  • Proven integrations with leading automation and LIMS platforms for partnered solutions

General Specifications

Dimensions (in) 8.6 (h) x 22.8 (w) x 15 (d)
Dimensions (cm)   22 (h) x 58 (w) x 38 (d)
Weight 30 lb (13.6 kg)
Power source 90-250 VAC, 50/60 Hz

Performance Specifications

Wavelength Range 340 - 850 nm
Wavelength Selection Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth 2 nm
Wavelength Accuracy < ± 1.0 nm
Wavelength Repeatability ± 0.2 nm
Photometric Range 0.0 to 4.0 OD
Photometric Accuracy < ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Precision < ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
Light Source Xenon flash lamp
Microplate Read Time - Endpoint 96-well: 9 seconds
384-well: 29 seconds
Microplate Read Time - Kinetic 96-well: 9 second interval
384-well: 29 second interval
Temperature Range Ambient + 4 °C up to 45 °C
Temperature Uniformity ± 0.5 °C @ 37 °C

SpectraMax® 340PC384 Microplate Reader


Part Number

SpectraMax 340PC384 Microplate Reader 340PC 384
 SpectraTest ABS1 Absorbance Validation Plate 0200-6117
StakMax Microplate Handling Stacker StakMax
Microplate Reader Shelf 9000-0756