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SpectraMax 340PC384 Microplate Reader

Capture 96- and 384-well microplate absorbance data that fuels your imagination


Be inspired by answers to an infinite range of research questions with the SpectraMax® 340PC384 Microplate Reader, our tunable visible absorbance reader for 96- and 384-well microplates. Acquire data in endpoint, kinetic, and spectral scan modes using wavelegths from 340-850 nm, tunable in 1 nm increments. Our SpectraMax® 340PC384 Microplate Reader comes with temperature control up to 45 °C, a robotics-compatible interface, and  SoftMax® Pro Data Analysis Software. PathCheck® Sensor technology automatically measures the liquid depth of each well, and can also be used to calculate concentrations without a standard curve and expand the dynamic range of your readings to 6+ OD. Our SpectraMax 340PC384 Microplate Reader will run so many of your applications, including ELISA, cytoxicity, ß- galatosidase reporter gene assays, and NAD(P)H-based assays.


  • No filter system: Includes monochromator allowing up to 6 wavelengths at a time in the visible wavelength range and scanning ranges from 340-850 nm in 1 nm increments
  • Easy assay optimization: Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine wavelength peaks
  • Correction for varying microplate well volumes and the elimination of standard curves based on PathCheck Technology, the only temperature-independent pathlength correction method
  • Thorough mixing of samples in microplate wells with user-programmable Automix feature
  • Comprehensive data analysis with SoftMax® Pro Software
  • Seamless integration with the StakMax® Microplate Handling Stacker

Microplate Reader Validation

The SpectraMax 340PC384 Microplate Reader supports a multitude of absorbance assay categories including:

  • Cell viability, proliferation, and cytotoxicity
  • ELISAs and immunoassays
  • Endotoxin detection
  • Enzyme assays
  • Microbial growth
  • Phosphotases/kinases
  • Protein quantitation
  • Reporter gene assays


SoftMax Pro Software simplifies setting up your assays by providing:

  • Simple set-up and automatic instrument recognition
  • Pre-defined protocols for endpoint and kinetic ELISA protein plus quantification assays such as Bradford, Lowry, BCA, and DC protein assays
  • A wide variety of scan types: Endpoint, Kinetic, Spectrum* and Well Scanning*
  • Standard data reduction settings are preselected as default
  • Automatic data recovery feature


SoftMax Pro Software offers a variety of customizing options:

  • Predefined calculation options for common data analysis functions    
  • Automatic export to Microsoft Excel
  • Instrument settings and plate read area can be adjusted to suit your experiment's needs
  • Custom data reduction settings can be selected for optimal signal selection
  • Full reports, with font flexibility, and live mini-tables and graphs are now available within the Notes section to simplify the result sharing experience      
  • Contemporary user community enabling protocol exchange and expertise
    sharing among our numerous users

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* available in select instrument models

General Specifications

Dimensions (in) 8.6 (h) x 22.8 (w) x 15 (d)
Dimensions (cm)   22 (h) x 58 (w) x 38 (d)
Weight 30 lb (13.6 kg)
Power source 90-250 VAC, 50/60 Hz

Performance Specifications

Wavelength Range 340 - 850 nm
Wavelength Selection Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth ≤ 2.0 nm
Wavelength Accuracy ± 1.0 nm
Wavelength Repeatability ± 0.2 nm
Photometric Range 0.0 to 4.0 OD
Photometric Resolution 0.001 OD
Photometric Accuracy < ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Precision < ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
Stray Light ≤ 0.05% at 340 nm
Light Source Xenon flash lamp
Microplate Read Time - Endpoint 96-well: 9 seconds
384-well: 29 seconds
Microplate Read Time - Kinetic 96-well: 9 second interval
384-well: 29 second interval
Temperature Range Ambient + 4 °C up to 45 °C
Temperature Uniformity ± 0.5 °C @ 37 °C

SpectraMax® 340PC384 Microplate Reader


Part Number

SpectraMax 340PC384 Microplate Reader 340PC 384
 SpectraTest ABS1 Absorbance Validation Plate 0200-6117
StakMax Microplate Handling Stacker StakMax
Microplate Reader Shelf 9000-0756

Number of Citations*: 7,630

Latest Citations:
For a complete list, please click here.

Ferulic acid protects PC12 neurons against hypoxia by inhibiting the p-MAPKs and COX-2 pathways

WC Lin, YF Peng, CW Hou - Iranian Journal of Basic Medical …, 2015 -
... Absorbance was recorded at 540 nm using SpectraMAX 340 reader. Data were
expressed as mean percentage of viable cells compared to control. ... Absorbance at
500 nm was determined using an ELISA reader (SpectraMAX 340). ...

Protective effect of a sesamin derivative, 3-bis (3-methoxybenzyl) butane-1, 4-diol on Aβ-stressed PC12 cells

CW Hou, SY Chang, KC Jeng - Archives of pharmacal research, 2015 - Springer
... solution, 200 ll of DMSO (dimethyl sulfoxide), was added to each well and absorption values
read at 540 nm on microtiter plate reader (spectraMAX 340, Molecular Devices, Sunnyvale, CA,
USA). ... Absorbance at 500 nm was determined using an ELISA reader (spectraMAX 340). ...

Independent and Co-morbid HIV Infection and Meth Use Disorders on Oxidative Stress Markers in the Cerebrospinal Fluid and Depressive Symptoms

J Panee, X Pang, S Munsaka, MJ Berry… - Journal of Neuroimmune …, 2015 - Springer
... Billerica, MA). The spectrophotometers used were SpectraMax 340 (for GSH,
HNE-His, and TBARS assays) and SpectraMax M3 (for GGT and GPx as- says) from
Molecular Devices (Sunnyvale, CA). GSH Assay (Pang et al. 2013) ...

Function Discovery and Structural Characterization of a Methylphosphonate Esterase

DF Xiang, YV Patskovsky, VV Nemmara, R Toro… - Biochemistry, 2015 - ACS Publications
... Metal Analysis The protein concentration was determined spectrophotometrically at
280 nm using a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis
spectrophotometer. The extinction coefficient at 280 nm for determination ...

Multiple Phosphatases Regulate Carbon Source-Dependent Germination and Primary Metabolism in Aspergillus nidulans

LJ de Assis, LNA Ries, M Savoldi… - G3: Genes| Genomes| …, 2015 -
... pH 7.0, 5 mM 2-mercapto ethanol, 1 mM MgCl 2 , 2 mM TPP, 5 mM alpha-ketoglutarate acid
disodium salt, 1 mM NAD + and 0.2 mM Coenzyme A). The generation of the reduced form of
NADH was measured at 340 nm using a SpectraMax I3 spectrometer (Molecular Devices). ...

Abnormalities in the Tricarboxylic Acid Cycle in Huntington Disease and in a Huntington Disease Mouse Model

NN Naseri, H Xu, J Bonica, JPG Vonsattel… - … of Neuropathology & …, 2015 -
Close Window. Close Window. Enter your Email address: Wolters Kluwer Health may email
you for journal alerts and information, but is committed to maintaining your privacy and will
not share your personal information without your express consent. ...

Impact of probiotics during weaning on the metabolic and inflammatory profile: follow-up at school age

F Karlsson Videhult, Y Andersson, I Öhlund… - … journal of food …, 2015 - Taylor & Francis
... Difference in absorbance between 590 nm and 450 nm was measured by SpectraMax 340
Microplate Reader (Molecular Devices, Sunnyvale, CA) and concentration of unknown samples
was calculated against standard with SoftMax Pro 2.6.1 software using 4-parameter ...

Emulsification and antioxidation of biosurfactant extracts from Chinese medicinal herbs fermentation in vitro

C Chen, T Lin, Y Shieh - Journal of bioscience and bioengineering, 2015 - Elsevier
... The absorbance at 540 nm was determined using a microplate reader (SpectraMax 340, Molecular
Devices, Sunnyvale, CA, USA). ... The absorbance at 450 nm or 570 nm was determined using a
microplate reader (SpectraMax 340 Molecular Devices, Sunnyvale, CA, USA). ...

Relationship between Inflammation and Oxidative Stress and Cognitive Decline in the Institutionalized Elderly

M Baierle, SN Nascimento, AM Moro… - … medicine and cellular …, 2015 -
... peroxidase (GPx) was measured according to the spectrophotometric method described previously
[23] and absorbance was monitored at 37°C in a microplate reader (SpectraMax M2, Molecular
Devices) at 340 nm for 6 minutes with readings every 20 s. GPx activity was ...

Effect of Deployment Time on Endotoxin and Allergen Exposure Assessment Using Electrostatic Dust Collectors

B Kilburg-Basnyat, N Metwali, PS Thorne - Annals of Occupational Hygiene, 2015 - BOHS
... 3595; Corning, Inc.) using a microplate reader (SpectraMax 340, Molecular Devices, Inc.) with
photometric measurements taken every 30 s for 90min at 405nm and 37°C. SoftMaxPro software
(Ver 4.7.1, Molecular Devices, Inc.) was used to analyse the data. ...



* as of June 26, 2015. Source: Google Scholar.