SpectraMax 190 Microplate Reader

UV-visible absorbance microplate reader for up to 96 samples with endpoint, kinetic and spectral scanning modes

The SpectraMax® 190 UV-Vis Microplate Spectrophotometer is ideal for life science applications, especially DNA analysis. The multi-channel design truly mimics a dual-beam spectrophotometer. Each sample has a discrete sample beam and reference beam so that each well is measured directly, eliminating error due to variations in light output between the optic fibers.

The patented multichannel optical design of the SpectraMax 190 Absorbance Microplate Reader mimics a dual beam spectrophotometer, measures each sample in the plate directly, and eliminates measurement error due to variations in light output between optical fibers on other instruments for more reliable data reporting.

Benefits

  • Eliminate the need for purchasing and changing out filters for wavelength optimization with a monochromator-based wavelength selection for UV-visible absorbance between 190 nm and 850 nm
  • Read up to 6 wavelengths in endpoint or kinetic mode for 96-well plates, and perform spectral scans to determine wavelength peaks
  • Measurement accuracy is increased due to narrow bandwidths that deliver increased and linearity over the widest range of assays, including DNA quantitation in the UV range
  • Better experimental signal discrimination using narrow absorbance bandpass for superior peak resolution and increased accuracy

Features

  • Ability to monitor temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
  • Correction for varying microplate well volumes and the elimination of standard curves based on PathCheck® Technology, the only temperature-independent pathlength correction method
  • Thorough mixing of samples in microplate wells by user-programmable Automix feature
  • Seamless automation coupling with the StakMax Stacker, Molecular Devices' own integrated microplate stacker designed exclusively for the SpectraMax Reader

Comprehensive Reader Validation

Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. Molecular Devices provides complete solutions with:

The SpectraMax 190 Microplate Reader supports a multitude of absorbance assay categories including:

  • Cell viability, proliferation, and cytotoxicity
  • ELISAs and immunoassays
  • Endotoxin detection
  • Enzyme assays
  • Microbial growth
  • Micro volume applications
  • Nucleic acid (DNA) quantitation
  • Phosphotases/kinasesrotein quantification
  • Reporter gene assays

SoftMax Pro Software, Molecular Devices industry leading all-in-one data acquisition and analysis software, offers:

  • The most extensive library of supported application protocols to get you started faster
  • Optional tools for regulated labs with SoftMax Pro GxP Compliance Software
  • Advanced calculations including non-linear fitting metrics for in-depth analysis
  • The only Mac- and Windows-native application support for seamless exchange of data files between operating systems
  • Proven integrations with leading automation and LIMS platforms for partnered solutions

General Specifications

Dimensions (in) 8.6 H x 22.8 W x 15 D
Dimensions (cm) 22 H x 58 W x 38 D
Weight 30 lbs (13/66 kg)
Power Source 90-250 VAC, 50/60 Hz

Performance Specifications

Wavelength Range 190-850 nm
Wavelength Selection Monochromator, tunable 1/0 nm increments
Wavelength Bandwidth 2 nm
Wavelength Accuracy < ± 2.0 nm
Wavelength Repeatability ± 2.0 nm
Photometric Range 0.0 to 4.0 OD
Photometric  Resolution 0.001 OD
Photometric Accuracy < ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Precision < ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
Stray Light < 0.05% @ 230 nm
Light Source Xenon flash lamp
Microplate Read Time --
Endpoint 12 seconds
Kinetic 9 second minimum interval
Temperature Range Ambient + 4 °C up to 45 °C
Temperature Uniformity ± 0.5 °C @ 37 °C

 SpectraMax® 190 Microplate Reader

Product

Product Number

SpectraMax 190 Microplate Reader with SoftMax Pro Software 190
SpectraTest ABS1 Absorbance Validation Plate 0200-6117
Quartz 96-well Microplate for UV Measurements R8024
Quartz 96-well Half-area Microplate for UV Mmeasurements R8028
SpectraDrop™ Micro-Volume Microplate Starter Kit 0200-6262
SpectraDrop™ Micro-Volume Microplate HTS Kit 0200-6267
StakMax Microplate Handling Stacker StakMax
Microplate Reader Shelf 9000-0756