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SpectraMax 190 Microplate Reader

A microplate reader for exploring the mysteries of life

Quantify DNA and perform endpoint, kinetic, and spectral scanning analyses with the UV-Vis SpectraMax 190 Microplate Reader. The multichannel design for 96-well microplates uses separate sample and reference beams for each sample — just like a dual-beam spectrophotometer — to eliminate error due to variations in optical fiber light output. Monochromator-based wavelength selection from 190 nm to 850 nm keeps you on the road to discovery, instead of tracking down and changing out filters.


The patented multichannel optical design of the SpectraMax 190 Absorbance Microplate Reader mimics a dual beam spectrophotometer, measures each sample in the plate directly, and eliminates measurement error due to variations in light output between optical fibers on other instruments for more reliable data reporting.


  • Eliminate the need for purchasing and changing out filters for wavelength optimization with a monochromator-based wavelength selection for UV-visible absorbance between 190 nm and 850 nm
  • Read up to 6 wavelengths in endpoint or kinetic mode for 96-well plates, and perform spectral scans to determine wavelength peaks
  • Measurement accuracy is increased due to narrow bandwidths that deliver increased and linearity over the widest range of assays, including DNA quantitation in the UV range
  • Better experimental signal discrimination using narrow absorbance bandpass for superior peak resolution and increased accuracy


  • Ability to monitor temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
  • Correction for varying microplate well volumes and the elimination of standard curves based on PathCheck® Technology, the only temperature-independent pathlength correction method
  • Thorough mixing of samples in microplate wells by user-programmable Automix feature
  • Seamless automation coupling with the StakMax Stacker, Molecular Devices' own integrated microplate stacker designed exclusively for the SpectraMax Reader

Comprehensive Reader Validation

Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. Molecular Devices provides complete solutions with:

The SpectraMax 190 Microplate Reader supports a multitude of absorbance assay categories including:

  • Cell viability, proliferation, and cytotoxicity
  • ELISAs and immunoassays
  • Endotoxin detection
  • Enzyme assays
  • Microbial growth
  • Micro volume applications
  • Nucleic acid (DNA) quantitation
  • Phosphotases/kinases
  • Protein quantification
  • Reporter gene assays

SoftMax Pro Software, Molecular Devices industry leading all-in-one data acquisition and analysis software, offers:

  • The most extensive library of supported application protocols to get you started faster
  • Optional tools for regulated labs with SoftMax Pro GxP Compliance Software
  • Advanced calculations including non-linear fitting metrics for in-depth analysis
  • The only Mac- and Windows-native application support for seamless exchange of data files between operating systems
  • Proven integrations with leading automation and LIMS platforms for partnered solutions

General Specifications

Dimensions (in) 8.6 H x 22.8 W x 15 D
Dimensions (cm) 22 H x 58 W x 38 D
Weight 30 lbs (13/66 kg)
Power Source 90-250 VAC, 50/60 Hz

Performance Specifications

Wavelength Range 190-850 nm
Wavelength Selection Monochromator, tunable 1/0 nm increments
Wavelength Bandwidth 2 nm
Wavelength Accuracy < ± 2.0 nm
Wavelength Repeatability ± 2.0 nm
Photometric Range 0.0 to 4.0 OD
Photometric  Resolution 0.001 OD
Photometric Accuracy < ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Precision < ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
Stray Light < 0.05% @ 230 nm
Light Source Xenon flash lamp
Microplate Read Time --
Endpoint 12 seconds
Kinetic 9 second minimum interval
Temperature Range Ambient + 4 °C up to 45 °C
Temperature Uniformity ± 0.5 °C @ 37 °C

 SpectraMax® 190 Microplate Reader


Product Number

SpectraMax 190 Microplate Reader with SoftMax Pro Software 190
SpectraTest ABS1 Absorbance Validation Plate 0200-6117
Quartz 96-well Microplate for UV Measurements R8024
Quartz 96-well Half-area Microplate for UV Mmeasurements R8028
SpectraDrop™ Micro-Volume Microplate Starter Kit 0200-6262
SpectraDrop™ Micro-Volume Microplate HTS Kit 0200-6267
StakMax Microplate Handling Stacker StakMax
Microplate Reader Shelf 9000-0756