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ImageXpress Ultra Confocal High-Content Analysis System

True-point source confocal high-content imaging

ImageXpress® Ultra System offers the ultimate solution when crisp, clear images are a priority. Controlled by state-of-the-art MetaXpress® Software, research-quality images of fixed- or live-cells are captured with high resolution. Diverse samples can be imaged with excellent signal-to-noise ratio, even when there is a high background, as in homogeneous, no-wash assays, or when samples are thick such as tissue sections or neurons growing in matrix.

  • True laser-scanning confocal system ensures a diverse set of samples can be imaged with excellent signal-to-noise ratio.
  • Software configurable detection pinhole provides two orders of magnitude of Z-resolution flexibility (from <1 µm to > 100 µm) over fixed-pinhole confocal systems.
  • Simultaneous or sequential scanning with up to 4 solid state lasers facilitates simultaneous acquisition for optimal speed performance (up to 4 times faster than single laser). Unique sequential scanning is achieved in combination with high-resolution z-offset to correct for objective lens-induced chromatic aberrations.
  • Fully-automated 100 nm resolution voice coil-driven stages provide positioning accuracy to capture intricate sub-cellular features as well as monitor long structures, such as neurons, across high-resolution tiled or stitched images.
neuron outgrowth imaging using high content confocal microscopy
Images of neurons were acquired and tiled from 6 sites. Each site is a long, continuous scan
capturing a larger area with long neuronal features. Note that the neurites perfectly
align across mutiple sites for easy quatification with our software algorithms.
  • Software configurable field-of-view simplifies imaging of large objects like model organisms (C. elegans and zebrafish) and long structures similar to neuronal processes.
     
long continuous scanning from high content confocal microscopy system
Long scan of cells stained with a cytosolic marker (green) and nucle (red).  
With a 20X objective scan lengths of up to 25.6 mm are possible.
  • Industry-leading autofocus option provides fast, reliable focusing with a dedicated 690 nm focus laser and ultra-fast sensors that will not bleach your sample while decreasing read times up to 5-fold.
  • Custom design for screening enables capturing 25,000 wells per day in a 384-well microplate assay with a two color high quality assay (e.g., Transfluor® beta-arrestin translocation assay). Lower resolution assays can be performed in excess of 100,000 wells / day.
  • 4-position automated objective changer eliminates manual objective changes and allows automated switching of magnifications during a single experiment.
  • Universal plate holder facilitates image acquisition using any clear bottom plate from 6-well to 1536-well, in standard height, low profile, or low volume.
  • Walk-away, 24/7 automation is achieved with the Thermo CRS CataLyst Express robotic plate handling system, enabling users to schedule multiple runs of one to three batches of 15 plates.

Screening

  • Determine the mechanisms of action for the research of cancer, neurological disorders, viral infection, and other diseases with compound or RNAi screening 
  • Quantitate multiplexed cellular responses, such as apoptosis, cell cycle, micronuclei, neurite outgrowth, angiogenesis, intracellular translocation, endocytosis, and cell signaling
  • Detect one to four fluorescent markers along with the ability to resolve their colocalization
Transfluor assay taken with confocal high content screening system
Unstimulated Transfluor-expressing cells wtih
cytosolic distribution of beta arrestin GFP.
Transfluor assay taken with confocal high content screening system
Stimulated Transfluor-expressing cells with
beta-arrestin GFP that has redistributed to vesicles.

Further Information

Visit the MetaXpress Software Application Modules to learn more about analysis for cell health, cell cycle, micronuclei, neurite outgrowth, angiogenesis, translocation, multi-wavelength cell scoring and granularity

Stem Cell Research

  • Gain insight into the complex molecular and genetic signaling events that underlie cell proliferation and differentiation
  • Accurately measure nuronal process across multiple fields of view using unique stitching algorithm with precise stage movements
  • Analyze 3D samples by capturing an image stack in the Z-plane within each field of view and collapse it into a best-focus image or view the sample in 3D

Further Information

Visit the MetaXpress Software Application Modules to learn more about analysis for neurite outgrowth, translocation, cell scoring and multi wavelength cell scoring.

Thick samples

  • Image neurons or stem cells grown on 3D matrices that can make focusing on the cells of interest a challenge  
  • Capture difficult-to-image thick tissue sections (at least 20 µm thick) that produce high-background fluorescence when stained
  • Analyze 3D samples by collecting a stack of crisp images in the Z-plane in as small as 100 nm increments
neuronal imaging assays analysed with high content analysis
Neuronal processes can be measured and
quantified with a Neurite Outgrowth Application
Module in MetaXpress Software.
laser scanning confocal system improves imaging of thick tissue sections
Mouse kidney section stained for
nuclei (blue), actin filaments (green) and
plasma membrane (red)

Further Information

Visit the MetaXpress Software Application Modules to learn more about analysis for neurite outgrowth and angiogenesis application modules.

Click here to learn more about MetaXpress Application Modules that enable some common HCS assays.  

Molecular Devices brings the image acquisition and analysis for ImageXpress Systems under one common platform to streamline the HCS workflow, offering a truly end-to-end solution for high-content screening.

MetaXpress® High-Content Image Acquisition & Analysis Software

  • MetaXpress Software Application Modules: turnkey solutions for your 100 most common analysis routines
  • MetaXpress Software Custom Modules: flexible toolbox for advanced assay development
  • MetaXpress Software Journals: powerful macros to analyze unique applications

AcuityXpress High-Content Informatics Software further analyzes, visualizes, and mines data.

MetaXpress® PowerCore High-Content Distributed Image Analysis Software dramatically accelerates analysis speed 25 times faster than acquisition in a high-throughput environment.

MDCStore Data Management Solution provides a secure access, management, and depolyment of a high-content analysis environment.

Stage

  • Linear-encoded voice-coil-driven X,Y, and Z stages have < 100 nm resolution

Objectives

  • Variety of Nikon 1x- 100x air- and oil- immersion lenses are supported.  

Lasers

  • The ImageXpress Ultra system is a Class 1 laser product.
  • The system comes standard with 488 nm and 635 nm solid-state lasers and a filter cube for GFP / DRAQ5 imaging.
  • Up to 2 additional solid state lasers can be added
    • Violet (405 nm)
    • Green (532 or 561 nm)
  • 690 nm wavelength laser for Autofocus

Emission filter specifications (vary by laser configuration)

Standard Configuration

PMT Excitation Laser (nm) Fluor Label Emission (nm) LP Dichroic (nm)
1
 
 
 
 
2
488
GFP
538/84
650
3
 
 
 
 
4
635
DRAQ5
692/40
 

532 nm Laser Filter Set Configuration

PMT Excitation Laser (nm) Fluor Label Emission (nm) LP Dichroic (nm)
1
405
DAPI
447/60
495
2
488
FITC
514/17
555
3
532
Cy3
585/40
650
4 635
Cy5
685/40
 

561 nm Laser Filter Set Configuration

PMT Excitation Laser (nm) Fluor Label Emission (nm) LP Dichroic (nm)
1
405
DAPI
447/60
495
2
488
FITC
525/50
555
3
561
Tex Red
593/40
650
4
635
Cy5
685/40
 

Compatible Dyes

405 nm Laser Excitation

Fluorophores Applications Detection Theoretically Possible with Filters
DAPI
DNA (nuclear) staining
Yes (447/60)
Hoecht 33342
DNA (nuclear) staining
Yes (447/60)
Alexa 405
Secondary antibody or streptavidin staining
Yes (447/60)
Alexa 430
Secondary antibody or streptavidin staining
Yes (525/50, others possible)
Cyan fluorescent protein (CFP) (not optimal for 405 laser)
Live cell protein dynamics, FRET with YFP
Yes (525/50, others possible)
Blue fluorescent protein (BFP)
Live cell protein dynamics
Yes (447/60)
Pacific Blue
Secondary antibody or streptavidin staining
Yes (447/60)
Lucifer Yellow
Neuronal tracing
Yes (525/50, others possible)
CellTrace calcein violet 405
Live cell identification
Yes (447/60)
LIVE/DEAD Fixable Violet Dead Cell Stain
Dead cell identification
Yes (447/60)
LIVE/DEAD Fixable Aqua Dead Cell Stain
Dead cell identification
Yes (525/50 , others possible)
Photoactivatable GFP (PA-GFP)
Live cell protein dynamics
Not Applicable (405 laser used for activation only)

488 nm Laser Excitation

Fluorophores Applications Detection Theoretically Possible with Filters
GFP
Live cell protein dynamics
Yes (514/17 or 525/50 or 538/84)
EGFP
Live cell protein dynamics
Yes (514/17 or 525/50 or 538/84)
FITC
Secondary antibody or streptavidrin staining
Yes (514/17 or 525/50 or 538/84)
Alexa 488
Secondary antibody or streptavidrin staining
Yes (514/17 or 525/50 or 538/84)
BODIPY FL
Coupled to various small compounds
Yes (514/17 or 525/50 or 538/84)
Calcein
Live cell identification
Yes (514/17 or 525/50 or 538/84)
Yellow fluorescent protein (YFP)
Live cell protein dynamics, FRET with CFP
Yes (525/50 or 538/84)
Oregon green
Fluorescein analog
Yes (514/17 or 525/50 or 538/84)
Rhodamine green
Secondary antibody or streptavidrin staining
Yes (514/17 or 525/50 or 538/84)
Acridine Orange
Dye (nuclear) staining
Yes (514/17 or 525/50 or 538/84)
7-AAD (7­Aminoactinomycin D)
Dye (nuclear) staining
Yes (685/40)
Ethidium Bromide
Dye (nuclear) staining
Yes (585/40 or 593/40)
SYTOX Green
Dye (nuclear) staining
Yes (514/17 or 525/50 or 538/84)
BCECF
Intracellular pH measurements
Yes (514/17 or 525/50 or 538/84)
Calcium Green
Intracellular Ca2+measurements
Yes (514/17 or 525/50 or 538/84)
Carboxy-SNARF (R)-1-AM pH 6
Intracellular pH measurements
Yes (585/40 or 593/40)
JC-1
Mitochrondrial membrane potential
Yes (585/40 or 593/40)
DiO
Membrane staining for cell tracking
Yes (514/17 or 525/50 or 538/84)
Fluo-3
Intracellular Ca2+measurements
Yes (514/17 or 525/50 or 538/84)
NBD-C6
 Golgi staining
Yes (514/17 or 525/50 or 538/84)

532 nm Laser Exciation

Fluorophores Applications Detection Theoretically Possible with Filters
Alexa 555
Secondary antibody or streptavidin staining
Yes (585/40)
Alexa 610-RPE
Secondary antibody or streptavidin staining
Possibly (585/40)
R-phycoerythrin (R-PE)
Secondary antibody or streptavidin staining
Yes (585/40)
Calcium Orange
Intracellular Ca2+measurements
Yes (585/40)
Dil
Membrane staining for cell tracking
Yes (585/40)
Propidium Iodide (PI)
DNA (nuclear) staining in dead cells
Yes (585/40)
POPO-3
DNA (nuclear) staining
Yes (585/40)
PO-PRO-3
DNA (nuclear) staining in dead cells
Yes (585/40)
TAMRA
Oligonucleotide labelling
Yes (585/40)
Ethidium homodimer (LIVE/DEAD)
DNA (nuclear) staining in dead cells
Yes (585/40)

561 nm Laser Excitation 

Fluorophores Applications Detection Theoretically Possible with Filters
TRITC
Secondary antibody or streptavidin staining
Yes (593/40)
Texas Red
Secondary antibody or streptavidin staining
Yes (593/40)
Mitotracker Red
Mitochondrial staining in live cells
Yes (593/40)
DsRed
Live cell protein dynamics
Yes (593/40)
Alexa 555
Secondary antibody or streptavidin staining
Yes (593/40)
Alexa 568
Secondary antibody or streptavidin staining
Yes (593/40)
Alexa 594
Secondary antibody or streptavidin staining
Yes (593/40)
R-phycoerythrin (R-PE)
Secondary antibody or streptavidin staining
Yes (593/40)
Dil
Membrane staining for cell tracking
Yes (593/40)
Calcium Crimson
Intracellular Ca2+measurements
Yes (593/40)
LysoTracker Red
Lysosome staining in live cells
Yes (593/40)
Rhodamine Red
Secondary antibody or streptavidin staining
Yes (593/40)
Nile Blue (not optimal)
Lipid staining in fixed cells
No
Alexa 610 (not optimal)
Secondary antibody or streptavidin staining
No
Alexa 610-RPE
Secondary antibody or streptavidin staining
No

635 nm Laser Excitation

Fluorophores Applications Detection Theoretically Possible with Filters
TO-PRO-3
 DNA (nuclear) staining in dead cells
Yes (685/40)
Alexa 633
Secondary antibody or streptavidin staining
Yes (685/40)
Alexa 635
Secondary antibody or streptavidin staining
Yes (685/40)
Alexa 647
Secondary antibody or streptavidin staining
Yes (685/40)
Cy5.5
Secondary antibody or streptavidin staining
Yes (685/40)
BODIPY 630/650
Protein conjugation
No
DiD
Membrane staining for cell tracking
Yes (685/40)
CellMask Deep Red
Plasma membrane staining
Yes (685/40)

Space and weight specifications

W (in) D (in) H (in) Weight (lb) W (cm) D (cm) H (cm) Weight (kg)
18.7
30.4
17.3
220
47.5
77.2
43.8
100

For product configurations and pricing on ImageXpress Ultra System, please please request a quote or contact us