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FLIPR Tetra High-Throughput Cellular Screening System

Industry-renowned, flexible, and reliable real-time kinetic cellular assay screening system for identifying early leads against GPCR and ion channel receptors

Quickly transform your creative concepts into real-time kinetic cellular assays for identifying early leads against GPCRs and ion channel targets. Our FLIPR Tetra® High-Throughput Cellular Screening System is fast, reliable, and remarkably easy to configure. The system is optimized for use with both fluorescence and luminescence, and adapts readily to your assay format with user-changeable 96-, 384-, and 1536-well heads.

Transform your ideas into fast, effective, cellular screening assays    

  • Configure and switch between fluorescence or luminescence read modes for assay flexibility
  • Multiple read modes support both single wavelength and ratiometric assays
  • Choice of fluorescent or dual-mode cameras (FL and LUM) for increased assay flexibility
  • User-changeable 96-, 384-, and 1536-pipettor heads easily change assay formats depending on your throughput needs
  • Configurable pipettor height and dispense speeds ensure proper mixing without disturbing cell monolayers or non-adherent cells
  • User-configurable optics supports rapid optic changes in a multi-user, multi-assay environment
  • Drag-and-drop software interface easily builds multiple step protocols for easy protocol definition
  • User-defined protocols can include up to four wash solvents to automate cleaning of cell handling system
  • Configurable data export in either time-sequence or well-based format of single or multiple data files enables analysis by Microsoft Excel, Spotfire, or other analysis program

Take Advantage of These Great Features

  • Fluorescence camera enables use of homogeneous FLIPR calcium and membrane potential kits
  • Contact based elastomeric liquid transfer for 1536 pipettor provides accurate pipetting of compounds and cells
  • Simultaneous pipet and read function enables fast kinetic assays such as calcium flux in multiple wells
  • Cell suspension system with adjustable stirring speed, pump, and pipetting parameters facilitates optimization with different cell types
  • Cell suspension system eliminates the need for separate compound plate during Aequorin assays
  • FLIPR TetraCycler Plate Handler enables microplate and pipette tip changes and facilitates integration to laboratory robotics systems
  • Software displays only options relevant to the instrument configuration and eliminates the opportunity to make protocol errors
  • User-defined protocols can include up to four wash solvents to automate cleaning of cell handling system
  • Viewing signal traces, EC50 curves, and Z factors on the fly provides quick data analysis without having to export data to another software program

Assay Kits

  • We offer a full line of assay kits optimized for use with the FLIPR Tetra System.

 

The FLIPR Tetra System supports numerous assay categories including:

  • GCPR assays: Calcium assay kits, Fura 2 QBT Calcium Assay Kit, LiveWare, Transfluor, cAMP assay
  • Ion channel assays: Membrane potential assay, Calcium assay
  • Enzyme assay: cGMP assay
  • Transporter assays: Neurotransmitter transporter assay, Fatty acid uptake assay
  • Cardiotoxicity assays

 

 

FLIPR Tetra System utilizes Molecular Devices ScreenWorks® System Control Software to define and run experimental protocols. Using a drag-and-drop interface, protocols can be easily set up to include:

  • Fluorescent or luminescent read modes
  • Simultaneous transfer of 96, 384, or 1536 liquid or cells in suspension
  • Complex quadrant, multiple aspirations, or multiple dispensing liquid handling
  • Single or ratiometric kinetic cell-based reading
  • Tip washing with up to two solvents
  • Cell suspension delivery with automatable cleaning protocols
  • Simple-to-create automation protocols

ScreenWorks Software is both flexible and user-friendly. During protocol set-up, only relevant options are displayed, based on the installed fluidics and optics hardware, making it easy to choose parameters that are right for each assay. Live and information-rich data in 96-, 384- or 1536-well plate format is displayed while testing. Specific wells can be grouped for analysis or data export purposes. Graphs and tables can be easily copied and pasted into documents, presentations or worksheets, and different reductions can be selected for group statistics, making results readily accessible.

General Specifications

 Dimensions (in.)
39 (W) x 70 (H) x 27 (D)
 Dimensions (cm)
99 (W) x 178 (H) x 69 (D)
 Weight (approx.)
850 lbs. (386 kg)
 Power consumption
600 VA
 Power Source
90-240 vAC, 50-60 Hz
 Operating system
Windows XP or Windows 7

Excitation Optics

 Source
Light-emitting diodes (LED) or no source
 Wavelength range*
335-626nm*
 Calcium (default)
470-495nm
 Membrane potential
510-545nm
 Voltage sensor probes
390-420nm
 Fura
335-345nm
380-390nm
 

* For complete details on the wavelengths addressed, please follow this link.

Emission Optics

Camera Type
EMCCD
ICCD
Detection
Fluorescence
Luminescence & Fluorescence
Mode
Cooled CCD camera
Intensified CCD camera:
 
Frame transfer operation
Regulated through software interface FI compatible with existing optics combinations*
Fluorescence sensitivity with Z' ≥ 0.5 (whole plate)
5 nM fluorescein
5 nM  fluorescein
Luminescence sensitivity
 
5,000 M5 AequoZen FroZen cells in 384-well format with Z' ≥ 0.5
Dynamic Range
 
4.8 log (1 measurement) 6.9 log (2 measurements)
Data acquisition rate
 7 Hz
8 Hz
Ratiometrics
Up to 4 EX/EM pairs per experiment
Up to 4 EX/EM pairs per experiment
Data acquisition rate for ratiometric emission
2 Hz (2EX/EM pairs)
2 Hz (2EX/EM pairs)

Emission Filters

3-position filter slider
 Filter wavelength ID
 Wavelength range*
 400-750 nm
 Calcium (default)
 515-575 nm
 Membrane potential
 565-625 nm
 Voltage sensor probes
 440-480 nm
 
 565-625 nm
 Fura
475-535 nm

* Based on product configuration

Fluidics

Simultaneous transfer
96, 384 and 1536
 
96 pipettor head
Range
 5-200 μL
 
 Precision @75 μL
 3% CV
 
 Pipetting increments
 1 μL
384-Well pipettor
Range
1-25 μL
 
Precision @ 25 μL
 4% CV
 
 Pipetting increments
 0.5 μL
1536-Well pipettor
Range
 0.5-3 μL
 
 Precision @ 3 μL
 6% CV
 
 Precision @ 1 μL
 10% CV
 
 Pipetting increments
 0.1 μL
 
 
 
 Quadrant transfer
 96 pipettor head
 384-well plate
 
 384 pipettor head
 1536-well plate

Plate Stage

Plate Stage:  Four positions in addition to tipwash station
 
 
Throughput†
 20 plates/hour
 
 (2-minute read time)
 96-well
 1,920 data points/hour
 384-well
 7,680 data points/hour
 1536-well
 30,720 data points/hour
 †1 read plate, 1 source plate and 1 tip rack
 
 Bar code manual mode
 Keyboard entry
 Bar code manual mode
 Short or long side
 Temperature control
 Ambient +5°C to 40°C

Cell Suspension System

This option consists of a user-installable cell reservoir and an external cell module for flask storage, light protection and mixing. Cells are re-circulated to the spinner flask to minimize cell volume used.
 
Cell reservoir
Autoclavable
 Compatible formats
96, 384, and 1536
 Stir flasks available
250 mL, 0.5 L, 1 L, 3 L

Related Collateral

Brochures/Datasheets

Applications

Posters

Publication


Related eBooks

Related Webinars

Product Part
Number
FLIPR Tetra High-Throughput Cellular Screening System FLIPR
Field Installations
FLIPR Tetra Camera Conversion Kit, EMCCD to ICCD 0310-5285
FLIPR Tetra Cell Suspension, Field Installation Kit 0310-5339
FLIPR Tetra TETRACycler Tetra+Cycler
Pipettor Heads
FLIPR Tetra Pipettor Head Kit, 96 0200-6071
FLIPR Tetra Pipettor Head Kit, 384 0200-6072
FLIPR Tetra Pipettor Head Kit, 1536 0200-6073
1FLIPR Tetra Pin Tool Head Kit, 384 384 Pin Tool Hd Kit
1FLIPR Tetra Pin Tool Head Kit, 1536 1536 Pin Tool Hd Kit
Pipetting Consumables
FLIPR Tetra Pipette tips, black, non-sterile, 96-well 50 racks/case 9000-0762
FLIPR Tetra Pipette tips, clear, non-sterile, 96-well 50 racks/case 9000-0761
FLIPR Tetra Pipette tips, black, non-sterile, 384-well 50 racks/case 9000-0764
FLIPR Tetra Pipette tips, clear, non-sterile, 384-well 50 racks/case 9000-0763
FLIPR Tetra 1536 tip gasket, non-sterile, 40/case 9000-0746
FLIPR Tetra 1536 tip block 0200-6112
1FLIPR Tetra Pin Tool head, 384 384 Pin Tool
1FLIPR Tetra Pin Tool head, 1536 1536 Pin Tool
Optical Accessories
LED Modules and Emission filter accessories are available for both versions of the FLIPR Tetra instrument for Calcium sensitive dyes, Membrane Potential dyes, Voltage Sensor dyes and others applications. Please follow this link to a .pdf of the wavelengths and ordering information
Optional Cell Suspension System  and Consumables
FLIPR Tetra Suspension Reservoir 0200-6222
FLIPR Tetra 250 mL Spinner Flask Assembly 0200-6223
FLIPR Tetra 500 mL Spinner Flask Assembly 0200-6224
FLIPR Tetra 1 L Spinner Flask Assembly 0200-6225
FLIPR Tetra 2 L Spinner Flask Assembly 0200-6226
FLIPR Tetra 3 L Spinner Flask Assembly 0200-6227

 1 Please contact your local Molecular Devices Sales Representative for details regarding this configure to-order item.

 FLIPR Tetracycler and Robotic Integration

fliprtetracycler
 
Optional Cell Suspension System  and Consumables
cellsuspensionsysgemdrawing
An optional cell management system for the FLIPR Tetra System simplifies luminescent calcium mobilization assays and increases throughput by eliminating the need to plate cells and the need for separate compound plates during aequorin assays. Cells are held in spinner flask which maintains a uniform cell suspension and promotes assay robustness. During the assay, cells are pumped to the reservoir for pipetting and returned to the spinner flask. Adjustable stir speed, pump speeds and pipetting parameters simplify the optimization of cell handling for different cell lines.  A variety of flask sizes are available to scale the volume for assay development through high throughput screening. Requires Part number 03010-5339 field installation of Cell Suspension system.
 

Number of Citations*: 9,980

Latest Citations:
For a complete list, please click here.

Dihydropyrrolopyrazol-6-one MCHR1 antagonists for the treatment of obesity: Insights on in vivo efficacy from a novel FLIPR assay setup

P Devasthale, W Wang, AS Hernandez, F Moore… - Bioorganic & Medicinal …, 2015 - Elsevier
Abstract Our investigation of the structure–activity and structure–liability relationships for 
dihydropyrrolopyrazol-6-one MCHR1 antagonists revealed that off-rate characteristics, 
inferred from potencies in a FLIPR assay following a 2 h incubation, can impact in vivo ...
 

Application of Parallel Multiparametric Cell-Based FLIPR Detection Assays for the Identification of Modulators of the Muscarinic Acetylcholine Receptor 4 (M4)

E Smith, P Chase, CM Niswender… - Journal of …, 2015 - jbx.sagepub.com
Abstract Muscarinic acetylcholine receptors (mAChRs) have long been viewed as viable 
targets for novel therapeutic agents for the treatment of Alzheimer's disease and other 
disorders involving impaired cognitive function. In an attempt to identify orthosteric and ...
 

Identification of MK-8133: An orexin-2 selective receptor antagonist with favorable development properties

SD Kuduk, JW Skudlarek, CN DiMarco… - Bioorganic & medicinal …, 2015 - Elsevier
... 2). 22 For example, compound 1, lacking the methylene linkage and possessing a
quinoline heterocycle, was found to be a very potent and highly OX 2 R SORA (FLIPR
IC 50 's: OX 1 R>10,000 nM; OX 2 R = 47 nM). The related ...
 

α-conotoxin MrIC is a biased agonist at α 7 nicotinic acetylcholine receptors

A Mueller, H Starobova, MC Inserra, AH Jin… - Biochemical …, 2015 - Elsevier
... Vic). FLIPR Tetra Calcium 4 No-Wash dye was from Molecular Devices (Sunnyvale,
CA, USA) and 384-well black-walled imaging plates were from Corning (Corning,
NY, USA). ... 2.3. FLIPR Tetra α7 nAChR assay. Functional ...
 

Synthesis, SAR and Pharmacological Characterization of Brain Penetrant P2X7 Receptor Antagonists

BM Savall, D Wu, M De Angelis… - ACS Medicinal …, 2015 - ACS Publications
... The first entry into this series of antagonists was compound 12a (Table 1), which had a potent
hP2X7 FLIPR IC 50 of 2.7 nM but was significantly less potent at the rat P2X7R with a IC 50 =
1900 nM. ... Human FLIPR pIC 50 measured in a Ca 2+ flux assay. Table b ...
 

In vitro pharmacological characterization of vorapaxar, a novel platelet thrombin receptor antagonist

BE Hawes, Y Zhai, D Hesk, M Wirth, H Wei… - European Journal of …, 2015 - Elsevier
... FLIPR assay in washed Human platelets and vascular smooth muscle cells. ... FLIPR buffer (HBSS
without phenol red, 20 mM Hepes, 0.04% Chaps, and 0.71 mg/ml Probenecid) supplemented
with Calcium 4 dye 40 µl/ml was then added to each well to produce a volume of 43 µl. ...
 

A selective α2B adrenoceptor agonist (A‐1262543) and duloxetine modulate nociceptive neurones in the medial prefrontal cortex, but not in the spinal cord of …

KL Chu, J Xu, J Frost, L Li, E Gomez… - … Journal of Pain, 2015 - Wiley Online Library
... FLIPR TETRA 384 assays. FLIPR TETRA 384 assay were performed using HEK cells
stably co-expressing the chimeric G αq/o protein with either rat α 2A , α 2B or α 2C
adrenoceptors. ... FLIPR TETRA 384 double addition assays. ...
 

Zinc‐Induced Effects on Osteoclastogenesis Involves Activation of Hyperpolarization‐Activated Cyclic Nucleotide Modulated Channels Via Changes in Membrane …

T Notomi, M Kuno, A Hiyama, K Ohura… - Journal of Bone and …, 2015 - Wiley Online Library
... Cells were loaded with FLIPR dye (FLIPR Membrane Assay Kit; Molecular Devices) in Hank's ...
and 5% CO2 in a humidified incubator. Membrane potential was recorded every 10 s for 8 min
by using the FLIPR Tetra system (Molecular Devices) at room temperature. To analyze ...
 

Dibenzazepines and dibenzoxazepines as sodium channel blockers

SM Lynch, L Tafesse, K Carlin, P Ghatak… - Bioorganic & medicinal …, 2015 - Elsevier
We have identified two related series of dibenzazepine and dibenzoxazepine sodium channel
blockers, which showed good potency on Nav1.7 in FLIPR-based and elect. ...
 

Bringing the light to high throughput screening: use of optogenetic tools for the development of recombinant cellular assays

V Agus, A Di Silvio, JF Rolland… - SPIE …, 2015 - proceedings.spiedigitallibrary.org
... if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate,
using the instrumentations normally dedicated to fluorescence analysis in High Throughput
Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate ...
 

 

* as of June 26, 2015. Source: Google Scholar.