Free webinars on applications for automated electrophysiology  View now >>

IonWorks Barracuda Plus Automated Patch Clamp System

For ion channel drug discovery from target validation to compound profiling

The IonWorks Barracuda® Plus System enables scientists to perform complex pharmacological analysis such as use-dependent blockade of ion channels, while exploiting the time and cost savings of automation. Now, a single platform provides an affordable, high-throughput solution for voltage- and ligand-gated ion channel research for drug discovery screening and safety assessment.

Benefits

  • Highest available throughput of 1100 to 6000 data points per hour
  • Low-cost IonWorks PatchPlate™ consumable to keep running costs down
  • Population Patch Clamp™ (PPC) recording for robust and reproducible recordings
  • Long assay windows, up to 30 minutes with stable ionic currents
  • Supports kinetic studies of use-dependent compounds


Replace slow, expensive methods

IWB_compounds.gif

days.gif

Relative cost comparison of consumables on the IonWorks Barracuda  system versus commercially available giga-seal systems.
Screen 25,000 compounds in 4 days on the IonWorks Barracuda system compared to approximately 106 days on commercially available giga-seal systems.

Replace manual methods

  • Easy to use - eliminates the need for labor intensive conventional patch clamp
  • Precise voltage control and high temporal resolution produces results similar to manual patch clamp methods
  • Long assay windows, up to 30 minutes, support the study of use-dependant blockers


Get the speed and benefits of automation at an affordable price

iwb_quoteCerne.gif

  • Peer reviewed studies show that IonWorks Barracuda recordings give comparable results to GigaSeal for most channels
  • Highest throughput available
    • True 384 parallel acquisition of an entire 384-well planar PatchPlateTMConsumable in as little as 20 minutes
    • Record up to 6000 data points per hour
  • Unmatched flexiblity for assay support and development
    • Optimized for both LGIC and VGIC
    • Add up to 8 compound additions per well - generate cumulative response data ideal for safety profiling
  • Automation solutions for walk-away operation up to six hours

Designed for accuracy and ease of use

  • Individual runs are fully automated
  • Easy access to plates and consumables
  • Unique cell delivery system minimizes cell usage - < 5 mL/run
  • Ultrasonic tip washer with two solution inputs eliminates compound carryover
  • CellPettor delivery of cell suspension minimizes on board cell usage when operating with or without optional cell management

iwb_deck.jpg

Instrument deck: Two boat positions, for extracellular buffer and cells, and two compound plates can be used for multiple additions. The wash station cleans both tips and the electrode plate and provides the user with the option for ultrasonic cleaning.

Proven, cost-effective PatchPlate™ consumables

  • Flow-through design enables rapid fluid exchange over the cell
  • Constant fluid level maitained for precise aspiration - use compound preparations up to 3x concentration
  • User-defined aspirate and dispense speed and height
  • Rapid individual offset correction for each of the 384 electrodes

patch-plate-dsipensing.jpg

 

At the forefront of developing ways to improve assay robustness and data quality Molecular Devices invented Population Patch Clamp™ (PPC) Technology, a patented, multiple hole-per-well technique that increases the experiment success rate and reduces well-to-well signal variability.

  • PPC substrate has 64 holes per well to enable seal formation and voltage clamp with up to 64 cells simultaneously

 

iwb_ppc.gif

PPC experiment of the acid-sensing ion channel ASIC1a showing how PPC generates highly reproducible data with low variability in 384 well-format
 

iwb_singlehole.gif

Single-hole experiment of the acid-sensing ion channel, ASIC1a, shows variability of signal amplitude commonly seen using conventional, single cell patch-clamping technique.

 

 

Primary screening of directed libraries

  • Speed and cost efficiency that supports screening 100's of thousands of compounds using one compound concentration per well


Rapid lead identification

  • 384-well format is ideal for generating large numbers of concentration response curves
  • High success rates with IC50 values from 95% or more of wells


Flexibility to optimize leads and perform safety studies

  • Wide assay windows
  • Software facilitates assay development - alter experimental conditions during runs similar to manual rigs
  • Multiple compound additions ideal for optimizing potency and eliminating more toxic compounds
  • Supports with-in well assays that generate cumulative response data similar to manual patch clamp methods


Clonal selection

  • Single hole recording is an option for identifying and optimizing cell lines

 

Voltage-gated ion channels

Many voltage-gated ion channels (VGIC) including Na+, K+, Cl-, and Ca2+ channels have been measured on IonWorks® systems. The same VGIC targets measured on the IonWorks HT and Quattro Systems can be measured on the IonWorks Barracuda System.

  • Deliver complicated voltage protocols to drive ion channels to steady state and to discover state-dependent drug candidates
  • Capable of performing within-well assays that generate cumulative response data similar to manual patch clamp methods
  • Numerous VGIC targets have been validated including: Na+, K+, Cl-, and Ca2+


Ligand-gated ion channels

  • Simultaneous compound addition and data acquisition allows the measurement of rapidly activating and desensitizing ligand-gated channels
  • Numerous LGIC targets have been validated including: ASIC1a, GABA, and nACh

Screening mode for day-to-day operation

  • Starting runs is a simple one step process - load a protocol and walk away
  • Easy to use intuitive interface


Assay development mode for protocol optimization

  • Alter experimental conditions during a run - quickly optimize protocols
  • Applied protocols can be changed an unlimited number of times
     

iwb_software.gif

Related Collateral

Brochure


Data Sheet


Applications

      
       Ligand-gated Ion Channels

          
       Voltage-gated Ion Channels

Poster

 

Related Webinars


Related Publications

Related Presentations

Description Part Number
PatchPlate (Single hole)
5008679
Patch Plate (PPC)
5008680
Pipette tips (clear)
5011975
Pipette tips (black)
5011976
CellPettor pipette
5012605
Standard cell boat
5005252
External buffer boat
5012604