The IonWorks Barracuda® Plus System enables scientists to perform complex pharmacological analysis such as use-dependent blockade of ion channels, while exploiting the time and cost savings of automation. Now, a single platform provides an affordable, high-throughput solution for voltage- and ligand-gated ion channel research for drug discovery screening and safety assessment.
- Highest available throughput of 1100 to 6000 data points per hour
- Low-cost IonWorks PatchPlate™ consumable to keep running costs down
- Population Patch Clamp™ (PPC) recording for robust and reproducible recordings
- Long assay windows, up to 30 minutes with stable ionic currents
- Supports kinetic studies of use-dependent compounds
Replace slow, expensive methods
Relative cost comparison of consumables on the IonWorks Barracuda system versus commercially available giga-seal systems.
Screen 25,000 compounds in 4 days on the IonWorks Barracuda system compared to approximately 106 days on commercially available giga-seal systems.
Replace manual methods
- Easy to use - eliminates the need for labor intensive conventional patch clamp
- Precise voltage control and high temporal resolution produces results similar to manual patch clamp methods
- Long assay windows, up to 30 minutes, support the study of use-dependant blockers
Get the speed and benefits of automation at an affordable price
- Peer reviewed studies show that IonWorks Barracuda recordings give comparable results to GigaSeal for most channels
- Highest throughput available
- True 384 parallel acquisition of an entire 384-well planar PatchPlateTMConsumable in as little as 20 minutes
- Record up to 6000 data points per hour
- Unmatched flexiblity for assay support and development
- Optimized for both LGIC and VGIC
- Add up to 8 compound additions per well - generate cumulative response data ideal for safety profiling
- Automation solutions for walk-away operation up to six hours
Designed for accuracy and ease of use
- Individual runs are fully automated
- Easy access to plates and consumables
- Unique cell delivery system minimizes cell usage - < 5 mL/run
- Ultrasonic tip washer with two solution inputs eliminates compound carryover
- CellPettor delivery of cell suspension minimizes on board cell usage when operating with or without optional cell management
Instrument deck: Two boat positions, for extracellular buffer and cells, and two compound plates can be used for multiple additions. The wash station cleans both tips and the electrode plate and provides the user with the option for ultrasonic cleaning.
Proven, cost-effective PatchPlate™ consumables
At the forefront of developing ways to improve assay robustness and data quality Molecular Devices invented Population Patch Clamp™ (PPC) Technology, a patented, multiple hole-per-well technique that increases the experiment success rate and reduces well-to-well signal variability.
- PPC substrate has 64 holes per well to enable seal formation and voltage clamp with up to 64 cells simultaneously
PPC experiment of the acid-sensing ion channel ASIC1a showing how PPC generates highly reproducible data with low variability in 384 well-format
Single-hole experiment of the acid-sensing ion channel, ASIC1a, shows variability of signal amplitude commonly seen using conventional, single cell patch-clamping technique.
Primary screening of directed libraries
- Speed and cost efficiency that supports screening 100's of thousands of compounds using one compound concentration per well
Rapid lead identification
- 384-well format is ideal for generating large numbers of concentration response curves
- High success rates with IC50 values from 95% or more of wells
Flexibility to optimize leads and perform safety studies
- Wide assay windows
- Software facilitates assay development - alter experimental conditions during runs similar to manual rigs
- Multiple compound additions ideal for optimizing potency and eliminating more toxic compounds
- Supports with-in well assays that generate cumulative response data similar to manual patch clamp methods
- Single hole recording is an option for identifying and optimizing cell lines
Voltage-gated ion channels
Many voltage-gated ion channels (VGIC) including Na+, K+, Cl-, and Ca2+ channels have been measured on IonWorks® systems. The same VGIC targets measured on the IonWorks HT and Quattro Systems can be measured on the IonWorks Barracuda System.
- Deliver complicated voltage protocols to drive ion channels to steady state and to discover state-dependent drug candidates
- Capable of performing within-well assays that generate cumulative response data similar to manual patch clamp methods
- Numerous VGIC targets have been validated including: Na+, K+, Cl-, and Ca2+
Ligand-gated ion channels
- Simultaneous compound addition and data acquisition allows the measurement of rapidly activating and desensitizing ligand-gated channels
- Numerous LGIC targets have been validated including: ASIC1a, GABA, and nACh
Screening mode for day-to-day operation
- Starting runs is a simple one step process - load a protocol and walk away
- Easy to use intuitive interface
Assay development mode for protocol optimization
- Alter experimental conditions during a run - quickly optimize protocols
- Applied protocols can be changed an unlimited number of times
- Characterization of Neurotoxin Modulation of Nav1.5 Channels on the IonWorks Barracuda Plus System
- Assay of ASIC1a Channel with the IonWorks Barracuda System
- Validation of the IonWorks Barracuda System for hERG Ion Channel Assay
Ligand-gated Ion Channels
- Rapid Solution Exchange for Recording of Fast-Desensitizing Ligand-Gated Ion Channels on the IonWorks Barracuda System
- Assay of ASIC1a Channel with the IonWorks Barracuda System
Voltage-gated Ion Channels
- Screening for Use-dependent Blockers of Nav1.5 Channels on the IonWorks Barracuda Automated Patch Clamp System
- Validation of a Multiple Compound Addition Assay for hERG Ion Channel on the IonWorks Barracuda System
- Ion channel compound profiling and GABAA receptor function
- Validation of a high-throughput, microfluidic patch clamp system for screening of nicotinic compounds
- Automated patch clamp in drug discovery: Anti-KV1.3 Nanobodies
- Assay development of ligand- and voltage-gated ion channels on automated patch clamp systems
- Developing Assays for Ion Channel Pain Targets and Peptide Neurotoxins on Automated Patch Clamp Systems - October 30, 2013
- Development of High Throughput Assays for Lead Optimization in Drug Discovery - September 18, 2013
- Development of Nicotinic and Sodium Channel Assays for Drug Discovery on the IonWorks Barracuda System - November 14, 2012
- Development of LGIC and VGIC Assays for Drug Discovery and Safety Profiling on the IonWorks Barracuda Platform - August 15, 2012
- Population Patch Clamp Improves Data Consistency and Success Rates in the Measurement of Ionic Currents
Finkel A, Wittel A, Yang N, Handran S, Hughes J, and Costantin J.
J Biomol Screen 11: 488-496, 2006 Jun 7.
- Novel 384-well Population Patch Clamp Electrophysiology Assays for Ca2+-activated K+ Channels
John VH, Dale TJ, Hollands EC, Chen MX, Partington L, Downie DL, Meadows HJ, and Trezise DJ.
J Biomol Screen 12: 50-60, 2006 Nov 7.
- Population Patch Clamp Electrophysiology: A Breakthrough Technology for Ion Channel Screening
Dale TJ, Townsend C, Hollands EC, and Trezise DJ.
Mol Biosyst 3: 714-722, 2007 Jun 15.
- Introduction to Next Generation Pain Therapeutics: Executing Nav Targets for Drug Discovery
Groppi, V. PhD., Vice-President, Chief Scientific Officer, Essen BioScience, MI, USA. May 2013
- Exploitation of IonWorks Barracuda Electrophysiology Platform to Improve Assessment of Cardiac Liability
Bridgland-Taylor, M., Discovery Sciences, AstraZeneca R&D, Alderly Park, UK. Ion Channel Retreat 2013
- Executing on Nav Drug Discovery with the IonWorks Barracuda Automated Electrophsiology Platform
Rock, D. Ph.D., Director, US Discovery Services, Essen BioScience. Ion Channel Retreat 2013
- Automated Patch Clamping for Screening and Profiling in Drug Discovery and Risk Assessment
Duzic, E. Ph.D., Senior Director, Discovery Services, ChanTest Corp. OH, USA. Ion Channel Retreat 2013
PatchPlate (Single hole)
Patch Plate (PPC)
Pipette tips (clear)
Pipette tips (black)
Standard cell boat
External buffer boat