CatchPoint cAMP Fluorescent Assay Kit

Cell signaling via G protein-coupled receptors (GPCRs) can be assessed by monitoring the downstream effectors calcium or Cyclic AMP (cAMP). Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of GPCRs.

CatchPoint cAMP Fluorescent Assay Kit

cAMP Fluorescent Assay Kit's high-affinity reagents are optimized for sensitivity and precision in applications where cAMP levels are low. A single wash step removes unbound material prior to the development step, so the assay is very resistant to interference from colored or fluorescent test compounds. The proprietary Stoplight Red substrate is used to generate a stable fluorescent readout, allowing the assay to be read in as little as 10 minutes or up to 24 hours after substrate addition.

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#R8088
  • each kit contains Two (2) 96-well black well/ clear bottom plates
  • pre-coated, pre-blocked and ready to use
  • 100 mL ELISA Assay Buffer
  • 1 vial Rabbit Anti-cAMP Antibody
  • 1 vial cAMP Calibrator
  • 1 vial HRP-cAMP Conjugate, 100 mL
  • 10X Wash Solution Concentrate
  • 50 mL Cell Lysis Buffer
  • 1 vial 100X Substrate
  • 100 mL Substrate Buffer
#R8089
  • each kit contains Ten (10) 96-well black well/ clear bottom plates
  • pre-coated, pre-blocked and ready to use
  • 950 mL ELISA Assay Buffer
  • 2 vials Rabbit Anti-cAMP Antibody
  • 2 vials cAMP Calibrator
  • 2 vials HRP-cAMP Conjugate
  • 950 mL 10X Wash Solution Concentrate
  • 450 mL Cell Lysis Buffer
  • 2 x 900 µL 100X Substrate
  • 950 mL Substrate Buffer
#R8053
  • each kit contains Ten (10) 384-well black well/ clear bottom plates
  • pre-coated, pre-blocked and ready to use
  • 2 x 950 mL ELISA Assay Buffer
  • 2 vials Rabbit Anti-cAMP Antibody
  • 2 vials cAMP Calibrator
  • 2 vials HRP-cAMP Conjugate
  • 950 mL 10X Wash Solution Concentrate
  • 2 x 450 mL Cell Lysis Buffer
  • 5 x 900 µL 100X Substrate
  • 950 mL Substrate Buffer
#R8044
  • each kit contains Two (2) 384-well black well/ clear bottom plates
  • pre-coated, pre-blocked and ready to use
  • 100 mL ELISA Assay Buffer
  • 2 vials Rabbit Anti-cAMP Antibody
  • 2 vials cAMP Calibrator
  • 2 vials HRP-cAMP Conjugate
  • 100 mL 10X Wash Solution Concentrate
  • 25 mL Cell Lysis Buffer
  • 1 vial 100X Substrate
  • 100 mL Substrate Buffer

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