Contaminant Detection Assays

Contamination detection assays can be used in a broad range of applications to measure contamination in a variety of sample content from fermentation supernatants and purification processes to biochemical preparations such as serum and other bodily fluids. They also measure the presence of host cell DNA as well as other DNA that might have been introduced during the biopharmaceutical manufacturing process.

Contaminant Detection Assays

Contaminant Detection Assays - Threshold is ideal for the quantitation of proteins, peptides and non-sequence specific total DNA.

It measures a wide range of analytes such as host cell proteins, bovine contaminants (BSA, IgG, transferring and insulin), Protein A and G, and total DNA at low concentrations. To simplify assay development, all reagents are supplied in pre-packaged kits to label your antibodies or commercially available antibodies. To optimize the interaction between the antigen and antibody, binding occurs freely in solution where the molecules are maintained in their native conformation and antibodies retain 100% of their activity. Assay sensitivity for proteins and peptides is typically equal to or better than radio immunoassays and at least 10X better than ELISA assays. When measuring DNA, sensitivity as low as 2 pg can be achieved. Typically assays have a dynamic range of 2 logs or greater, so fewer sample dilutions are required for accurate quantitation. Assays are fast and are complete in as little as 90 seconds.

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threshold-contamination-detection-total-dna-assay-and-immuno-ligand-assay
#R9010
* includes: (1) Total DNA Assay Kit, (100) Sarstedt 2 ml test tubes, (100) biopure pipette tips 1 ml, (100) biopure pipette tips 200
#R9004 DNA BULK KIT
* includes: (104) Threshold Sticks, (20) Threshold Blank Sticks, (66) Zero Calibrator, (20) Positive Control, (20) High Calibrator, (100) DNA Labeling Reagents (13) Reconstitution Buffer, (10) Substrate Concentrate, (19) Wash Solutions, (13) Filter Unit Kits (104 units)
#R9009 DNA KIT
* includes: (10) Threshold Sticks, (6) Zero Calibrator, (2) Positive Control, (2) High Calibrator, (8) DNA Labeling Reagents, (1) Reconstitution Buffer, (1) Substrate Concentrate, (2) Wash Solutions, (1) Filter Unit Kits (8 units)
#R9003
* includes: (1) Universal Standard Reagent, (6) Enzyme Reagent, (1) Capture Reagent, (6) Threshold Blank Sticks, (24) Threshold Sticks, (1) Substrate, (1) Assay Buffer Concentrate, (1) Wash Buffer Concentrate
#R9002
* includes: 2 vials Biotin label (5 mg/vial), 2 vials Fluorescein label (5 mg/vial)

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