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Clampfit 10 Feature List

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General Features

  • Messaging for Automated Analysis - Clampfit can receive a message from Clampex indicating that a new data file has just been written. Clampfit can be configured for automated analysis of files as they become available, such as the automatic generation of I-V plots.
  • Open multiple Analysis windows simultaneously
  • Hide/Unhide signals from display
  • User-configurable toolbars with pre-defined toolbuttons for all menu items
  • Session activities and analysis results can be automatically saved to a log file or printed
  • Extensive on-line help
  • Context-sensitive right-mouse-button menus
  • Fully configurable Analysis window parameters: fonts, colors, X and Y axis ticks, titles
  • Click-and-drag zoom in X and Y axes
  • Data exported as Axon binary or ASCII text files for use with other programs

Analysis Window

  • Process large data sets (2 gigasamples)
  • Decimate data files for export
  • Work on selected sweeps or whole data files
  • Next/Previous feature for rapid review of sequentially acquired data files
  • Nine cursors per Analysis window, with mouse control and keyboard shortcuts for moving cursors
  • Event Detection:
    • Template Event Detection:
      • A sliding template matches event shapes.
      • Template can scale in amplitude to match the event.
    • Threshold Event Detection:
      • Up to 9 categories, where amplitude levels serve as triggers.
      • Includes re-arm and rejection levels for each category.
      • Pretrigger and post-trigger length selection.
      • Calculate taus by fitting found events.
    • Single-Channel Event Detection.
    • Event Viewer window to display captured events.
    • Dynamic plotting from Results window
  • Event Analyses:
    • Burst Analysis expanded.
    • Latency Analysis.
    • Perievent Analysis for timing before and after tagged events.
    • P(open) on continuous and episodic files.
    • Watch histograms being built while events are being detected.
    • Linked data views between the Analysis, Results and Graph windows.
  • Additional Analyses (Analysis Window):
    • Nonstationary Fluctuation Analysis.
    • V-M (Quantal) Analysis.
    • Burst Analysis.
    • Histograms.
    • Trace Normalization.
    • Control File Subtraction.
    • Auto-correlation.
    • Cross-correlation.
  • Filter dialog
  • Filtering:
    • High-pass: 8-pole Bessel or single-pole RC
    • Low-pass: boxcar, Butterworth, Chebyshev, Gaussian, 8-coincident-pole RC, single-pole RC
    • Band-pass: same as above
    • Notch
    • Electrical interference: 50 or 60 cycle noise, up to 20 harmonics
  • Fit dialog
  • Fitting:
    • 37 predefined fitting functions
    • User-defined custom fitting functions
    • Model comparisons & graphical seeding
    • Fit up to 6 terms
    • Fitting statistics
    • Fitted curve, components and/or residuals display
  • Power Spectrum:
    • Window types: none (rectangular), Bartlett (triangular), Blackman, Blackman-Harris 3, Blackman Harris 4, Cosine, Hamming, Kaiser, Poisson, Van Hann
    • Power Spectrum dialog
    • Average trace spectra
    • Average spectral segments
    • RMS measurement and plot
    • Graphical representation of time domain and spectral magnitude
  • Additional Features:
    • File Concatenation.
    • Locked cursor pairs.
    • Difference measurements in cursor box.
    • Up to 8 Statistics Search Regions.
    • User-set percentile ranges for Rise/Decay Statistics.
    • Clampex-style statistics display.
    • Statistics search region mean slope measurement based on regression.
    • Statistics rise and decay mean slope measurements based on regression.
  • Baseline adjustment, leak subtraction
  • Resistance measurements (passive steady-state
    membrane) on voltage-clamp or current-clamp experiments
  • Shape statistics
  • Average traces or files
  • Custom arithmetic expressions
  • Cursor measurements
  • Data reduction
  • Data interpolation
  • Force values
  • Time shift

Results Window

  • Automatic analyses written to pre-defined results sheets: Cursors, Basic Statistics, Shape Statistics, Resistance, Fitting Parameters, Graphs, Power Spectrum.
  • Time formats: Elapsed from start of file, time of day, stopwatch time
  • Column arithmetic
  • Correlations
  • Analysis of variance
  • Nonparametric statistics
  • Create Data dialog
  • F-test
  • Student's t-Test
  • Breakdown analysis with binning
  • Create, sort & transpose data
  • Column graphing
  • Extract Data Subset numeric filter.
  • Average Columns.
  • Kolmogorov-Smirnov Test.
  • Rank Correlations.
  • Auto- and Cross-correlation.

Graph Window

  • Automatic on-line I-V graphs
  • "Quick" I-V or trace graphs
  • Axes styles: zero-crossing, linear, log, linear-log, log-linear
  • Plot styles: line, scatter, histogram
  • Rectangular Scatter Plot selection tool.
  • Graphs saved with the Results Window data.
  • Full Cursor statistics for cursor pairs 1 & 2, and 3 & 4.
  • 1 million row limit.

Layout Window

  • Paste data, results and plots
  • Grid array or free-form positioning
  • Annotations and objects: text, arrows, lines, rectangles, polygons, ellipses

Lab Book Window

  • Procedures are automatically logged
  • Comments can be added to the log. Full text editing and searches.
  • Graphics (such as screen shots) can be pasted into the log

Data File Index (DFI)

Data File Index
  • The DFI is a database for organizing data files and sorting them by categories: File name, Acquisition mode, Date, Time of day, Sampling rates, Experiment Type, ID numbers, use of Leak Subtraction, User list, Conditioning train, External tags, Statistical Analyses or Math signal, Number of sweeps, Holding potentials, File Comments, Input and Output signal names, and the path to the data file.
  • The DFI can be sorted on any category and files searched for with any text value in any category.
  • Files are added to the DFI by data file search criteria, including folder name, dates of acquisition, acquisition modes, and data file comment.

Data Transfer

  • Data in all types of windows can be saved or exported in the Axon file formats ABF (2.0 and 1.8) or ATF, and gap-free data can be exported from the Analysis Window in LDT format (QuB).
  • Segments of data defined by cursors can be saved.
  • You can copy screen images to the Windows Clipboard either as bitmaps or in metafile format. Vector-based, the metafile format is excellent for transferring screen images to a word-processing or drawing program, allowing you to resize the image or edit data objects in the image.

Display Options

Analysis window Properties
  • View data as overlapped sweeps, concatenated sweeps, or continuous (elapsed time, including gaps where no data were recorded)
  • Invert axes
  • Customizable toolbars
  • Color and font control of data and window components
  • Blank data
  • Display scaling: full scale X, Y, or all axes, auto-scale Y or all Y axes, zoom into data or axes