Computer Requirements
- Intel® Pentium-4 processor or later
- Microsoft® Windows® XP
- CD-ROM drive
- 512MB or more system memory (RAM) (more memory may be required for processing large image data sets)
- 200MB free hard disk space for program only (image storage requires more space)
- 24-bit graphics display
Acquisition
- Up to five wavelengths per cycle
- Real time background subtraction (independent background for each wavelength)
- Real time shading correction (independent shading reference for each wavelength)
- Time lapse
Automation
- Control for multiple shutters, filter wheels, monochromators and other wavelength-changing devices
- Device triggers for pumps, valves, strobes or flash lamps using TTL outputs
- Customizable journals and taskbars
Digital Camera Acquisition Features
(depends on imaging hardware used)
- Exposure time, gain, A/D transfer speed, bits-per-pixel for each wavelength
- On-chip gain multiplication
- Binning and sub-region selection
- Control of integrated camera shutter
- Support for frame transfer, interline, full frame, back illuminated sensors
- Streaming of data for high speed applications
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Video-Based Acquisition Features
(depends on imaging hardware used)
- RS-170 or CCIR video inputs
- Frame averaging, on-chip camera integration, summation into a 16-bit buffer
- Analog gain and black level offsets for each wavelength
- Adjustable intensifier gain for each wavelength
- Compensation for camera lag
Calibrations
- Grynkiewicz equation
- Titration calibrations with choice of curve fits
- Calibration maps to directly display pH, calcium or other ion concentrations
Analysis
- Ratio of up to two indicators per cycle
- Automatic generation of multiple regions of interest
- Fluorescence Resonance Energy Transfer
- Multiple graphs display
- Event Marks and image annotation
- Tracking of experiment conditions
- IMD, pseudocolor or monochrome display
- QuickTime® or AVI formats for movies
- Data logging to text file or spreadsheet such as Microsoft® Excel®
- Compatible with MetaMorph®
Custom Configuration
- Multi-user environment available
- Settings storage for each type of experiment
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