AutoScanXT

Automated Image Analysis for the ArcturusXT Microdissection Instrument

The AutoScanXT Image Analysis Software Module is an image analysis program for the ArcturusXT microdissection instrument that automatically identifies areas for microdissection based on user-defined criteria. This software module has been is simple and straightforward to use, and the automated identification of cells greatly reduces the overall time required to perform a microdissection experiment. The AutoScanXT Image Analysis Software Module performs optimally on high contrast samples and may be used with colorimetric, fluorescence and IHC stained specimens.

With basic software controls, the user first selects a few typical regions of interest (ROI’s) and background areas, and then AutoScanXT Image Analysis Software Module employs these parameters to automatically identify areas in the sample for microdissection. Learned AutoScanXT Image Analysis Software Module analysis files can be saved and used subsequently on other areas from the same sample or on other similarly stained tissue samples. The user may choose from a variety of algorithms depending on the complexity of the sample and may extend the analysis to include the interrogation of sample texture and morphology.

Once the regions have been automatically identified, the user may proceed to standard microdissection using the ArcturusXT™ Microdissection Instrument. Cells of interest are captured using the CapSure® LCM Caps, providing ultimate quality control with the opportunity for complete inspection and documentation.

Human Breast Carcinoma: IHC with Cytokeratin Antibody and DAB detection

Step 1: User Marks Representative Regions of Interest (ROI) & Background	Step 2: AutoScanXT Scans and Identifies Areas for Microdissection (Green)	Step 3: User Selects Items to be Microdissected (Blue)

	Step 4: Post-Microdissection on the ArcturusXT Instrument	Step 5: Microdissected Material on the CapSure® LCM Cap

Human Breast Carcinoma: Immunofluorescence with Cytokeratin Antibody and Cy3 Detection

Step 1: User Marks Representative Regions of Interest (ROI) & Background	Step 2: AutoScanXT Scans and Identifies Areas for Microdissection (Green)	Step 3: User Selects Items to be Microdissected (Blue)

Step 4: Post-Microdissection on the ArcturusXT Instrument		Step 5: Microdissected Material on the CapSure® LCM Cap