Paradise® Plus Reagent System
Complete Solution for Gene Expression Analysis
The
Paradise® Plus Reagent System from Arcturus enables unprecedented
gene expression analysis of millions of previously inaccessible
samples. For the first time, you can measure whole genome expression
profiles in formalin-fixed, paraffin-embedded (FFPE) tissue samples.
Unlock the Tissue Archives
Currently,
tens of millions of tissue samples with known outcomes are available
in tissue archives. The Paradise® Plus Reagent system enables microarray and qRT-PCR analysis of these currently inaccessible samples, allowing retrospective
studies to accelerate discoveries.
- Open
new opportunities — perform gene expression experiments
using archived tissue blocks
- Use
single sections from tissue blocks and preserve the valuable remainder
- Leverage
archived samples to expand experimental designs
- Use
samples prepared following standard fixation protocols
- Work
directly with application specialists dedicated to your success
Achieve Unprecedented Results in Six Steps
The
Paradise® Plus Reagent System is an integrated system enabling gene expression
studies using Formalin Fixed Paraffin Embedded (FFPE) tissue.
Integrate
Seamlessly into Current Workflow
The Paradise® PlusReagent System is compatible with
all major microarray platforms.
Robust and reproducible results are achieved using tools and techniques
commonly
employed in today’s research laboratories.
Paradise® Plus reproducibility on GeneChip®
Human Genome X3P array (Affymetrix). Amplification was
performed on FFPE breast cancer samples. The double-stranded
cDNA generated was used as template in the Enzo® High Yield™ Transcript Labeling Kit. Duplicate samples were
hybridized to GeneChip® microarrays, resulting in
intensity correlation of R=0.983.
Paradise® Plus reproducibility on cDNA arrays. Two-round amplifications were performed on independent
laser captured FFPE tissue cells. Amplified aRNA was labeled during
reverse transcription and hybridized to two cDNA arrays, resulting
in correlation of gene expression ratios, R= 0.993 (log2 ratios).
Paradise® Plus reproducibility on Agilent
microarrays (Agilent Technologies). Two-round amplifications
were performed on independent laser captured human breast cells.
Labeled aRNA was hybridized to custom-designed Agilent microarrays,
resulting in correlation of gene expression ratios, R=0.986 (log2 ratios).
Additionally, amplification
across a variety of tissues is robust, providing microgram quantities
of aRNA suitable for labeling and hybridization to microarrays.
Samples
processed using the Paradise® Plus Reagent System. Starting with
5,000-10,000 laser captured cells produces amplified RNA sufficient
for duplicate microarray hybridizations.
Secure Downstream Success with the Paradise® QC Kit
Not all FFPE samples contain high quality
RNA. Identify the best samples for your studies using a simple,
real-time PCR research technique. Proceed with confidence using
these pre-qualified samples.
Benefit from the Only Complete System for Formalin-fixed Samples
The Paradise® Plus Reagent System provides all the components necessary to prepare RNA from formalin-fixed, paraffin-embedded tissue samples for gene expression analysis. To ensure successful microarray results, the system includes reagents optimized for exceptional recovery of RNA and superior RNA amplification.
Arcturus has validated the Paradise® Plus Reagent System as a key component of the only proven platform for microgenomics. This platform includes the ArcturusXT™ Microdissection instrument, the most advanced technology for high-throughput isolation of pure cell populations.
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