The binding solution conditions for the validated IMAP substrates are optimized to ensure the finest performance when using the IMAP platform.
Substrates come in two sizes and can be used with both IMAP FP and IMAP TR-FRET.
To date, we have validated dozens of different substrate sequences with over 100 kinases, and additional calibrator peptides.
Substrates are available with both red and green labels, enabling multiplexing and providing a way to address issues with compound autofluorescence interference, present in some compound libraries.
To facilitate the customization of the IMAP assay to fit your specific needs, Molecular Devices offers a wide range
of validated substrates and calibrators. The IMAP substrates, which have been optimized for maximum performance,
are offered individually as a complement to the IMAP Screening Express and IMAP Purchase Plan (IPP). The IMAP
Screening Express and IPP programs allow greater flexibility by providing the detection portion of the IMAP assay,
leaving the enzymatic reaction to be determined by the researcher. These substrates may be used for the enzymes
for which they were tested or as potential substrates for alternate enzymes. The tables below provide a list of
the substrates, their amino acid sequences, and the part numbers.
