IMAP Red Dyes

In cases where a compound library has a high level of auto fluorescence, use of red-labeled substrates can reduce fluorescence interference. In addition, red-labeled substrates can be used in combination with green-labeled substrates for multiplexing.

IMAP has been shown to work with a wide variety of red dyes. Some examples are Cye3b and Cye5 from Amersham, Alexa dyes, cAMP from Perkin Elmer/Molecular Probes, and several California Dyes from Biosearch. However, MDC decided to focus on TAMRA as the preferred red dye for its excellent performance and its universal availability.

Fluorescence Polarization Assays of Kinases and Phosphatases with Red-Labeled Peptide Substrates [PDF]   (click below)

Multiplexing with IMAP [PDF]   (click below)