IMAP FP and TR-FRET Progressive Binding System

The Progressive Binding System enables the researcher to achieve maximum performance for every possible application when using IMAP. It can be used with virtually every kinase, phosphatase and phosphodiesterase. It provides the assay development team with the ability to easily address key variables such as substrate choice, desirable ATP concentrations and background parameters.

IMAP FP Configuration & Optimization  [PDF]

Reagent	Description
IMAP Progressive Binding Buffer A	Baseline Binding Buffer
IMAP Progressive Binding Buffer B	Affects TR-FRET background by reducing, or "blocking", the non-phosphate-based binding of the fluorescent substrate to the Binding Reagent.
IMAP Progressive Binding Reagent	Introduces the phosphate binding entities. This Binding Reagent specifically binds to phosphate residues via a coordinate covalent complex bond.
IMAP TR-FRET Donor (=Tb based Phospho conjugate)	Enables the TR-FRET read-out based on the close proximity of Tb-Donor and fluorophor on phosphorylated substrate by binding both even under very stringent Binding Buffer conditions to the Binding entities.

Component proportions are adjusted according to the acidic character of the substrate.