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FLIPR Calcium Assay Kit

Cell Lines Successfully Used

Adherent

Receptor

CHO

 

Engineered GPCRs
M1 (muscarinic)
NCR (nociceptin)
Glutamate
Human adrenoceptor
Recombinant human 5-HT subtypes
Adenosine subtypes

HEK293

 

VIP1 (vasoactive intestinal peptide)
AT1a
Nicotinic subtypes
Mu-opiod/chimeric G-protein
Serotonin

1321 N1 astrocytoma cell line
stable transfectants*

 

Ligand-gated calcium channel

C3H/An stable transfectant

 

Human adrenoceptor alpha 1

COS7

   

A10

   

HTC

   

A7R5

   

U373

   

SH-SY5Y

   

Non-adherent

THP-1

Jurkat

T cells

PC-12

Primary rat hepatocytes

Additional cell lines

Immortalized human cortical stem cells

Immortalized human striatal stem cells

Immortalized human ventral stem cells

Immortalized human mesencephalon stem cells

Immortalized human cerebral stem cells

Immortalized human hippocampal stem cells

Non-invertebrate cell line (cannot disclose cell type)