CatchPoint cAMP Fluorescent Assay Kit
Reagents for cyclic-AMP Detection
Ultimate sensitivity in a HTS format
- Sensitive detection limit: 6 fmol cAMP/well
- Robust format, resists interference from compounds
- Rapid signal development (< 10 min)
- No termination step, flexible read time
- Compatible with high throughput screening (HTS)
- Detection limit: 24 fmol/well for 96 well format
- Detection limit: 12 fmol/well for 384 well format
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Introduction
The CatchPoint cyclic-AMP Assay Kit measures cAMP levels and adenylate cyclase activity via a competitive
immunoassay format. The assay's high affinity reagents are optimized for sensitivity and precision in applications
where cAMP levels are low. In an HTS setting, the assay minimizes the handling steps and timing constraints of
traditional immunoassays.
In contrast to homogeneous formats, the assay is very resistant to interference from colored or fluorescent compounds.
The compounds are washed away prior to the development step, and due to the fluorescent signal's intensity, small
traces of residual fluorescence or signal quenching are insignificant.
Sensitive
Many high throughput assays fail to produce viable data when cAMP levels are low. Screening agonists / antagonists of
Gi-coupled GPCRs can be particularly problematic. The CatchPoint® cAMP Assay Kit's single wash step significantly
helps increase resolution and improve data quality in these situations.
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96-Well Format |
384-Well Format |
Detection
Limit: |
0.2 nM (24 fmol/well) |
0.1 nM (6 fmol/well) |
Maximum
Measurable
Concentration: |
120 nM (14 pmol/well) |
80 nM (4800 pmol/well) |
EC50 |
2.8 ± 0.7 nM (340 fmol) |
3.4 ± 0.6 nM (204 fmol) |
Convenient
With only a single wash step, the cAMP Assay Kit is easy to use. There are no multiple wash steps and no radioactive
components as required by other sensitive assay procedures. The protocol is straightforward and user-friendly. The
assay is read by fluorescence intensity measurement and has been validated on both Analyst® Family instruments and
the SpectraMax Gemini XS System, both from Molecular Devices.
Flexible read time
The CatchPoint cAMP Assay Kit uses the proprietary Stoplight Red™ substrate to generate a stable and precise
fluorescent readout without requiring exact timing in its incubation stages.
Using Stoplight Red, no termination step is needed to halt the assay, so the assay can be read in as little as
10 minutes or up to 24 hours after substrate addition. This makes the assay particularly suitable for robotic or
workstation procedures that are prone to variations in timing.
Assay performance
Calibration curve: the calibration curve for the CatchPoint cAMP Assay Kit was generated on the Analyst detection
system. Data was taken 10 minutes after addition of Stoplight Red substrate. The EC50 of the cAMP
calibration curve was 2.1 nM (126 fmol). Minimum detectable concentration was 0.1 nM (8 fmol).
Product Number |
R8088 |
R8044 |
Product Name |
CatchPoint® cAMP 96-well Explorer Fluorescent Assay Kit |
CatchPoint® cAMP 384-well Explorer Fluorescent Assay Kit |
Unit Size |
1 Explorer Kit provides:
2, 96-well black clear-bottom plates
100 mL ELISA Assay Buffer
1 vial Rabbit Anti-cAMP Antibody
1 vial cAMP Calibrator
1 vial HRP-cAMP Conjugate
100mL, 10X Wash Solution Concentrate
50 mL Cell Lysis Buffer
1 vial 100X Substrate
100 mL Substrate Buffer
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1 Explorer Kit provides:
2, 384-well black clear-bottom plates
100 mL ELISA Assay Buffer
2 vials Rabbit Anti-cAMP Antibody
2 vials cAMP Calibrator
2 vials HRP-cAMP Conjugate
100mL, 10X Wash Solution Concentrate
25 mL Cell Lysis Buffer
1 vial 100X Substrate
100 mL Substrate Buffer
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Product Number |
R8089 |
R8053 |
Product Name |
CatchPoint® cAMP 96-well Bulk Fluorescent Assay Kit |
CatchPoint® cAMP 384-well Bulk Fluorescent Assay Kit |
Unit Size |
1 Bulk Kit provides:
10, 96-well black clear-bottom plates
950 mL ELISA Assay Buffer
2 vials Rabbit Anti-cAMP Antibody
2 vials cAMP Calibrator
2 vials HRP-cAMP Conjugate
950mL, 10X Wash Solution Concentrate
450 mL Cell Lysis Buffer
2 x 900 µL100X Substrate
950 mL Substrate Buffer
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1 Bulk Kit provides:
20, 384-well black ELISA plates
2 x 950 mL cAMP Assay Buffer
2 vials Rabbit Anti-cAMP Antibody (lyophilized)
2 vials cAMP Calibrator
2 vials HRP-cAMP Conjugate
950 mL, 10X Wash Solution Concentrate
2 x 450 mL Cell Lysis Buffer
5 x 900 µL100X Substrate
950 mL Substrate Buffer
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To place an order, call your local Molecular Devices Office or Molecular Devices Distributor.
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