Application Webinar:
Permeability Measurement with Double-Sink™ PAMPA Using SpectraMax® Microplate Readers

	This webinar was broadcast on February 3rd, 2010. Please click HERE for a recording of this webinar.

Cathy Olsen, Ph.D., Application Scientist, Molecular Devices
Yvonne Beirne FitzGerald, Field Application Scientist, Molecular Devices
Dana C. Lipp, Product Specialist, pION INC

Double-Sink PAMPA is highly correlated with the permeability of drugs through Caco-2 cells (passive), human jejunum (GIT) and the blood brain barrier (BBB). With this microplate-based method that is performed using SpectraMax Microplate Readers, drugs can be evaluated and screened much earlier in development, thus saving resources for only the most promising candidates. The other, more costly screens can be employed later in the process when they are most cost effective.

In this webinar, we will present early PAMPA models and the advantages of more recent biomimetic models. You will learn about the important properties of drug adsorption of which permeability is one important aspect, and how the permeability of your compound should be consistent with other physicochemical properties. We will discuss the advantages of using SpectraMax Microplate Readers to generate accurate data. The presentation will cover important considerations in permeability measurement and interpreting results to effectively focus scarce resources.

Topics to be covered include:

• Generating data with SpectraMax Microplate Readers
• Importance of pH in drug absorption
• Early models: black lipid membrane
• GIT and BBB biomimetic considerations
• What is the ABL and why should I care?
• Low solubility compounds: a special case
• Correlation with Caco-2, GIT and BBB
• Application of permeability data vs BCS
• Developing new models

	Please check back regularly for information on upcoming events, including user meetings and web seminars. Or contact Candace.Anderson@moldev.com if you have questions.