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Microplate Readers: Assays Compared

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This assay list is comprehensive but not complete. It is provided to assist you in understanding assay performance by specific readers. You may find application notes on these assays by clicking on a specific microplate reader at the top of the chart, and on the product page click on the Literature link in the right menu to see a list of application notes available.

  Spectra
Max M5e
Spectra
Max M5
Spectra
Max M2e
Spectra
Max M2
FlexStation
3
Gemini EM Gemini XPS SpectraMax L SpectraMax Plus384 Spectra
Max 190
SpectraMax 340PC384 VersaMax Vmax Emax
Endpoint Assays bullet
Kinetic assays
Well Scanning                
Spectrum Scanning          
ELISA/immunoassays
protein assays:  
Bradford, BCA, Lowry      
Protein (A280)              
NanoOrange, CBQCA              
nucleic acid assays:  
DNA/RNA/Oligos (A260)                
RiboGreen, PicoGreen, Hoechst, OliGreen              
DNAquant                          
SNP genotyping              
molecular beacons              
reporter genes:  
alk. phos., beta-galactosidase
beta-lactamase            
luciferase/dual luciferase                    
GFP                  
cell viability/proliferation/death:  
MTT, XTT, Crystal Violet, SRB, Alamar Blue  
LDH/dehydrogenase            
fluorescent Live/Dead kits, Alamar Blue              
calcein AM, CyQUANT              
SYTOX Green, ethidium homodimer              
enzyme assays:  
superoxide (cytochrome c)          
protease activity    
microtubule association                
coagulation factors            
proteases    
NADH      
GST    
Fast Kinetic  
Ca mobilization                            
Ion channel                            
Transporter assays bullet bullet bullet bullet bullet bullet bullet              
Phosphotases/Kinases      
microbial growth            
endotoxin endpoint colorimetric      
other:  
nitric oxide (nitrate/nitrite)  
cell adhesion    
FRET                
ADME-TOX            
HTRF®                        
Delfia/TR-FRET                      
PathCheck:  
pipettor calibration
(to 4 µL)
             
automatic correction
to 1 cm