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Microplate Readers: Assays Compared

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This assay list is comprehensive but not complete. It is provided to assist you in understanding assay performance by specific readers. You may find application notes on these assays by clicking on a specific microplate reader at the top of the chart, and on the product page click on the Literature link in the right menu to see a list of application notes available.

  Spectra
Max M5e
Spectra
Max M5
Spectra
Max M2e
Spectra
Max M2
FlexStation
3
Analyst GT Gemini EM Gemini XPS SpectraMax L SpectraMax Plus384 Spectra
Max 190
SpectraMax 340PC384 VersaMax Vmax Emax
Endpoint Assays bullet
Kinetic assays bullet
Well Scanning bullet                
Spectrum Scanning bullet          
ELISA/immunoassays bullet
protein assays:  
Bradford, BCA, Lowry bullet      
Protein (A280) bullet              
NanoOrange, CBQCA bullet              
nucleic acid assays:  
DNA/RNA/Oligos (A260) bullet                
RiboGreen, PicoGreen, Hoechst, OliGreen bullet              
DNAquant                            
SNP genotyping bullet              
molecular beacons bullet              
reporter genes:  
alk. phos., beta-galactosidase bullet
beta-lactamase bullet            
luciferase/dual luciferase     bullet                
GFP   bullet                
cell viability/proliferation/death:  
MTT, XTT, Crystal Violet, SRB, Alamar Blue bullet  
LDH/dehydrogenase bullet            
fluorescent Live/Dead kits, Alamar Blue bullet              
calcein AM, CyQUANT bullet              
SYTOX Green, ethidium homodimer bullet              
enzyme assays:  
superoxide (cytochrome c) bullet          
protease activity bullet    
microtubule association bullet                
coagulation factors bullet            
proteases bullet    
NADH bullet      
GST bullet    
Fast Kinetic  
Ca mobilization         bullet                    
Ion channel         bullet                    
Transporter assays bullet bullet bullet bullet bullet bullet bullet bullet              
Phosphotases/Kinases bullet      
microbial growth bullet            
endotoxin endpoint colorimetric bullet      
other:  
nitric oxide (nitrate/nitrite) bullet  
cell adhesion bullet    
FRET bullrt                
ADME-TOX bullet            
HTRF®       dot                  
Delfia/TR-FRET     bullet                  
PathCheck:  
pipettor calibration
(to 4 µL)
bullet              
automatic correction
to 1 cm
bullet