MultiWash III Microplate Washer
A 96-well strip washer for ELISA, EIA, FIA, and cytotoxicity assays
- Simple
- Flexible
- Dependable
- Low Cost
- Compact
The Molecular Devices MultiWash III is a low-cost, 96-well microplate strip washer that represents the perfect balance between features and affordable pricing. The MultiWash III can be used to perform a wide range of assays including ELISA (Enzyme-Linked ImmunoSorbent Assay), EIA (Enzyme Immuno Assay), FIA (Fluorescent Immuno Assays), LIA (Luminescent Immuno Assay), Cytotoxicity Assays and Agglutination tests in flat, round or V-bottom microplates. The system is completely self-contained, and can goes from out-of-the-box to full operation in just minutes, performing a 300 µL three-cycle wash on a 96-well plate in less than two minutes.
Features
The MultiWash III incorporates unique design features such as color-coded quick connect fittings (Figure 1), single-button cleaning, and easy programming. The soft-touch keys on the display pad (Figure 2) are used to create and
store up to 50 user-defined programs, making the MultiWash III a reliable choice for ease of operation and dependability. The syringe dispense system provides complete control of fluid flow and aspiration, and the coaxial needle design (Figure 3) allows both bottom-washing and over-flow washing capabilities (Figure 4), providing maximum flexibility in washing both cell-based and biochemical assays.
Flexibility
The MultiWash III has a positive-displacement dispense pump that assures accurate and consistent dispensing. Coaxial manifolds are available in 8-port, 12-port, or dual 8-port configurations to dispense volumes from a range of 50-1990 µL in row, column, or double-column format. Aspiration from the microplate wells is by the use of a built-in vacuum pump, eliminating the need for additional equipment on the floor or lab bench.
Dependability
To ensure reliability, the instrument performs a maintenance rinse after every eight hours of non-usage to prevent wash solutions from drying up and leaving salt deposits between uses. The waste reservoir is fitted with a liquid level sensor to indicate if the reservoir is full. Only the tips of the aspiration needles make contact with liquid in the microplate wells, so crosscontamination and carry-over are minimized.
Technical Specifications
Performance Specifications |
| Resolution |
10 µL |
| Accuracy |
±3% @ 300 µL |
| Precision |
3% CV @ 300 µL |
| Processing time (full plate, 3 cycles, 300 µL/well) |
| 8-port manifold: |
180 sec. |
| 12-port manifold: |
120 sec. |
| 2-8-port manifold: |
90 sec. |
|
| Dispense volume per well |
| 8-port manifold: |
50-1990 µL |
| 12-port manifold: |
50-1650 µL |
| 2-8-port manifold: |
50-1250 µL |
|
| Residual aspiration volume |
<3 µL per well |
| Maximum soak time |
999 sec. |
| Maximum wash cycles |
10 |
| Liquid contact materials |
Delrin®, Glass, Nylon, Polypropylene Tygon®, Teflon®, Polyethylene,
Santoprene, and Stainless Steel |
General Specifications |
| Dimensions (in.) |
9.5 (H) x 11 (W) x 14.5 (D) |
| Dimensions (cm) |
24 (H) x 28 (W) x 37 (D) |
| Weight (instrument) |
12 lbs. 5.4 kg |
| Power requirements |
99-132 Vac/198-264 Vac, 50-60 Hz |
| Power consumption |
30 watts max. |
| Operating temperature |
10-40°C |
| Storage temperature |
1-40°C |
| Fluid reservoir capacity |
2 L each for Wash, Rinse, and Waste |
|