Assays “Powered by SpectraMax^™”

Molecular Devices® GPCR, Ion-Channel, Membrane Transporter, and Kinase targeted assays

Molecular Devices, now part of Molecular Devices, is well-known as a leader in providing novel, high-performance assay solutions optimized for Molecular Devices' instrumentation.

GPCRs

Kinases, phosphatases, and phosphodiesterases

The IMAP® platform for screening kinases, phosphatases, and phosphodiesterases, has a variety of tools and products such as the Evaluation kits, IMAP Purchase Plan, Substrate Finder kits and Validated Substrates that have all been validated on the SpectraMax® M5/M5e, FlexStation® 3 and Analyst® GT.

Ion Channels

The FLIPR Membrane Potential assay kit measuring ion-channel activity has been validated on the FlexStation II and FlexStation 3.

Membrane Transporters

Our membrane transporter assay kits, Fatty Acid Uptake and Neurotransmitter Transporter Uptake are validated on the SpectraMax, Gemini, FlexStation and Analyst GT readers.

**For current reagent and plate reader promotions please visit our promotions page.

If you have any questions about our reagent assays, please contact us at reagents@moldev.com, or visit our reagent homepage for more information.

Below you will find links to various protocols, application notes, and product posters for Molecular Devices reagent assays with microplate readers.

SoftMax® Pro Data Analysis Protocols

• CatchPoint cAMP
• FLIPR Calcium
• FLIPR Membrane Potential
• IMAP Evaluation Demo Kit
• IMAP FP FAM
• IMAP FP TAMRA
• IMAP TR-FRET TAMRA
• Neurotransmitter Uptake
• QBT Fatty Acid Uptake - Endpoint
• QBT Fatty Acid Uptake - Kinetic

Product Insert protocols

• CatchPoint cAMP Fluorescent Assay Kit (384-well Explorer format)
• FLIPR Calcium Evaluation Kit
• FLIPR Calcium Assay Kit
• FLIPR Calcium 3 Assay Kit
• FLIPR Calcium 4 Assay Kit
• FLIPR Membrane Potential Explorer Kit
• QBT Fatty Acid Uptake Assay Kit
• Neurotransmitter Transporter Uptake Assay Kit
• IMAP Evaluation Demo Kit
• IMAP FP Phosphodiesterase Evaluation Assay Kit
• IMAP TR-FRET Phosphodiesterase Evaluation Assay Kit
• IMAP FP with Progressive Binding Systems
• IMAP TR-FRET with Progressive Binding Systems
• IMAP Substrates for FP and TR-FRET
• IMAP FP Substrate Finder for Serine/Theronine Kinases 2
• IMAP FP Substrate Finder for Tyrosine Kinases

Application Notes

• A Stable, Sensitive Fluorescence-based method for Measuring Cyclic AMP using the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #46)
• A Stable, Sensitive Fluorescence-based Method for Measuring Cyclic GMP (MaxLine Application Note #52)
• The Progressive Binding System (IMAP Application Note #1)
• Detection and Quantification of Kinase Inhibitors (IMAP Application Note #2)
• Determination of Ki and Km values in IMAP Assays (IMAP Application Note #3)
• Developing Calibration Curves for IMAP (IMAP Application Note #4)
• Multiplexing with IMAP (IMAP Application Note #5)
• Setting up a Kinase Profiler (IMAP Application Note #6)
• TR-FRET Detection with IMAP (IMAP Application Note #7)

Product Posters

• A Stable, Sensitive Fluorescence-based method for Detecting Cyclic AMP
• Quantification of fatty acid uptake in HepG2 cells and adipocytes using the QBT™ Fatty Acid Uptake Assay
• Comparison of Calcium Flux Assays Across Multiple GPCRs: Implications for Compound Profiling and Screening
• Homogeneous Fluorescence Analysis of Cellular Fatty Acid Transport
• Quantification of Fatty Acid Uptake in HepG2 cells in adipocytes using the QBT™ Fatty Acid Uptake Assay
• A Fluorescence-Based Neurotransmitter Transporter Uptake Assay
• Comparison of the IMAP Fluorescence Polarisation Assay with the Scintillation Proximity Assay for Phosphodiesterase Activity
• Fluorescence Polarization Assays of Kinases and Phosphatases with Red-Labeled Peptide Substrates
• IMAP® Assays of Protein Kinases: Determination of Kinetic and Inhibition Constants at High Substrate Conversion
• Non-Antibody Based Fluorescent Polarization Assay for Monitoring Kinase Activities Optimized on Analyst®, A Multimode Fluorescent Detection System
• A Comparison of FRET and FP Assay Platforms: Z’-LYTE and IMAP
• Advances in High Throughput Kinase Assays via Fluorescence Polarization
• CHARACTERIZATION OF KINASE SUBSTRATE SPECIFICITY USING IMAP: Application for the CDK Family
• Expansion of IMAP to Assays Requiring High-ATP and Use of Substrates With A High Proportion of Acidic Residues.
• Fluorescence Polarization Assays of Proteases with the IMAP Platform
• FP and TR-FRET kinase assays with automated liquid handling
• High Density Kinase Screening and Selectivity Determination Using the IMAP Platform
• IMAP TR-FRET: Use of the First Generic TR-FRET based Kinase Assay Platform with Protein Substrates and Biotin-Substrate/Streptavidin Systems
• IMAP™ Kinase and Phosphatase Assays in Automated High-Throughput Applications
• Kinase Profiler with IMAP
• Kinase Profiling Studies at Roche: The Establishment of a Twenty Kinase Profiler and Its Application
• LOPAC™ screen for PDE1 using the IMAP® platform with TR-FRET detection
• Multiplex Assays in IMAP Using the Progressive Binding System
• Multiplexing and Cascade Assays with the IMAP FP Platform
• Setting up a Kinase Profiler with IMAP
• The IMAP Substrate Finder for Tyrosine Kinases: Applications for HTS Assay Development
• The IMAP Substrate Finder: Applications for HTS assay development
• The Use of the Substrate Finder for Ser/Thr Kinases in Development of IMAP Assays for the PKC Family
• Time-resolved Fluorescence Resonance Energy Transfer detection for Lipid kinases using the IMAP(R) platform: Non-radioactive and antibody independent assay for Sphingosine Kinase
• TR-FRET Detection for the IMAP Platform
• Use of IMAP TR-FRET detection mode for IMAP Substrate Finders
• Use of IMAP TR-FRET detection mode for the IMAP AGC and CAMK Substrate Finder
• Using IMAP Technology to Identify ATP Non-Competitive Kinase Inhibitors in 1536-well Format

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