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Using the GenePix 4000 for Immunohistochemistry

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Figure 1.  Mouse embryo, whole mount paraffin section. β-tubulin revealed by Cyanine 3 TSA Plus™ detection. Specimen courtesy of Dr. Kevin A. Roth.



Figure 2.  Rat brain coronal section. 200 MW Neurofilament subunit (NF200, shown in yellow) revealed by Cyanine 3 and Neuronal nuclear antigen (NeuN, shown in blue) revealed by cyanine 5 TSA Plus™ detection. Specimen courtesy of Dr. Kevin A. Roth.



Figure 3.  Rat brain coronal section. Cyclooxygenase-2 (COX-2, shown in green) revealed by Cyanine 3 and Neuronal nuclear antigen (NeuN, shown in red) revealed by cyanine 5 TSA Plus™ detection. Specimen courtesy of Dr. Kevin A. Roth.



Figure 4.  Rat brain coronal section. Glial fibrillary acid protein (GFAP, shown in blue) revealed by Cyanine 3 and Microtubule-associated protein-2 (MAP2, shown in green) revealed by cyanine 5 TSA Plus™ detection. Specimen courtesy of Dr. Kevin A. Roth.



Figure 5.  Mouse brain coronal cryostat section. Intermediate-phosphorylated neurofilament (SMI-31, shown in green) revealed by Cyanine 3 and Cathepsin-D (shown in red) revealed by cyanine 5 TSA Plus™ detection. Specimen courtesy of Dr. Kevin A. Roth.