FlexStation II

A Spectrum of Flexibility

Functional cell assays on your benchtop, results within minutes

FlexStation® II offers the highest level of flexibility in developing functional cellular assays and analyzing real time kinetic data for 96-well format.

Whether you are screening a few hundred to a thousand compounds or simply need an assay development tool for Molecular Devices FLIPR workstation, FlexStation will satisfy those needs and more. FlexStation is a benchtop scanning fluorometer that provides both top- or bottom-read functions with the click of a mouse. The dual monochromator design offers the ability to perform ratiometric and multiplex applications.

FlexStation's integrated 8- channel pipettor, which is able to transfer compounds from one microplate to the assay plate, allows prompt kinetic assays such as calcium flux, membrane potential or oxidative burst. Furthermore, the integrated fluidics platform allows precise compound transfer using consumable pipette tips to completely avoid cross-contamination.

Assay Development / Lead Optimization

• Increase your throughput and maximize flexibility in developing functional cellular assays.
• No large spatial requirements. Its footprint is no larger than that of a plate reader!
• Simplify transitioning your assays directly to and from the FLIPR® workstation.

Multi-channel Compound Transfer

• Multi-compound screening.
• Eliminate contamination from one user to next by simply changing pipette tip racks.
• Automixing and titration functions enable proper mixing of even the smallest amounts of transferred compounds.

Integrated Dual Scanning Monochromator with Fluidics Assay Optimization

Optimize both excitation and emission wavelengths with the dual monochromator optical bench design and our unique spectral scanning feature that will help you define exact excitation and emission wavelengths. No need to settle for predefined filters, we put the control in your hands.

Real-time Kinetic Data from Functional Assays!

• Total integration of optics, fluidics, and temperature control.
• Ideal for homogeneous, kinetic, cell-based fluorometric assays, such as measuring intracellular calcium, membrane potential, and intracellular pH.

SoftMax® Pro Software

FlexStation™ II is controlled by Molecular Devices' industry-leading SoftMax® Pro software for data acquisition and analysis. SoftMax Pro enables you to format assays and create protocols in seconds.

Software-controlled experimental parameters include:
Reagent transfer volumes and dispensing speeds Reagent addition times and mixing intervals Temperature control Data sampling intervals Choice of single or multiple emmission or excitation wavelength settings Well scanning and spectrum scanning	Click image for larger view

Specifications

Fluorescence Photometric
Emission Wavelength Range	360 - 850 nm
Excitation Wavelength Range	250 - 850 nm
Scanning Increments	1 nm
Sensitivity (Signal 3X STD DEV) Top Read (96-well plate)	3 fmol/well FITC
Sensitivity (Signal 3X STD DEV) Bottom Read (96-well plate)	8 fmol/well FITC*
Sensitivity (Signal 3X STD DEV) Top Read (384-well plate)	3 fmol/well FITC
Sensitivity (Signal 3X STD DEV) Bottom Read (384-well plate)	6 fmol/well FITC*
Time-Resolved Fluorescence (384-well plate)	0.5 fmol/well Eu-Ch*
Luminescence Photometric
Sensitivity	10 amol ALK Phos*
Wavelength Range	360 - 850 nm
General Photometric
Microplate Formats	6-, 12-, 24-, 48-, 96-, and 384-well
Detection Spot Size: Bottom Read	1.5 mm
Detection Spot Size: Top Read	3.5 mm
Lamp Life	Exp. 2 years, warr. 1 year
Mechanical
Read Time (Seconds)	< 9 (96 wells), < 29 (384 wells)
Single Column Read Frequency (96-Well)	1 Hz
Single Well Read Time Frequency	20 Hz
Shaker Time	0 to 999 seconds
Dimensions (cm)	58 cm x 40 cm x 49 cm (W x D x H)
Thermal (Read Chamber and Fluidics)
Temperature Chamber Range/Rate	Ambient + 1 °C to 45 °C in 30 minutes
Fluidics (96- & 384-well Plates Only)
Maximum Volume — 8 Channels	200 µl
Precision @ 50 µl — 8 Channels	2% CV
Precision @ 5 µl — 8 Channels	8% CV
Maximum Dispense Rate — 8 Channels	208 µl/sec
Electrical
Power Consumption	450 VA
Line Voltage and Frequency	90-240 VAC, 50-60 Hz

* specifications subject to change

Consumables

Item	Part Number
FlexStation Calcium Assay Kit	R-8041
FlexStation Membrane Potential Assay Kit	R-8042
96-well, FlexStation Pipette tips, Black, 10 Racks/Box	9000-0911
96-well, FlexStation Pipette tips, Clear, 10 Racks/Box	9000-0912
96-well, FlexStation Pipette tips, Black, 10 Racks/Box	9000-0622
96-well, FlexStation Pipette tips, Clear, 10 Racks/Box	9000-0623

** Tips can be purchased in 0.1 case quantities = 10 Racks/Box

To place an order, call your local Molecular Devices Representative.