| |
IonWorks
Quattro |
PatchXpress
7000A |
OpusXpress
6000A |
| Applications |
Primary screening of directed libraries, secondary screening, lead optimization, early toxicology screens |
Compound profiling, lead optimization, structure-activity relationship studies, cardiac safety assessment |
lead optimization, basic research, target discovery |
| Electrode Type |
Planar |
Planar |
Pipette |
| Substrate |
Polyimide |
Glass |
Glass |
| Recording Chambers |
384 |
16 |
8 |
| Patching Technology |
Single-hole (SH) and Population Patch Clamp (PPC) |
Single-Hole Planar Patch Clamp |
Two-Electrode Voltage Clamp |
| Recording Configuration |
Perforated Patch Clamp |
Whole-Cell Patch Clamp |
Two-Electrode Voltage Clamp |
| Typical Success Rate |
PPC: >95% |
50% – 90% |
90% – 100% |
| Cell Type |
CHO, HEK, CHL, NIH3T3, BHK, L929, NG108, Rin5F, SF9, and others |
HEK, CHO, CHL, RBL, L(tk), SH-SY5Y, GH4C1, LNCaP, SJ-RH30, PC12, 3T3 and others |
Oocytes |
Compatible Ion-
Channel Types |
Voltage-Gated and Slowly Desensitizing/Activating Ligand-Gated Ion Channels |
Voltage-Gated and Rapidly Desensitizing Ligand-Gated |
Voltage- and Ligand-Gated Ion Channels |
| Seal Resistance |
0.05 - 1.0 GΩ |
1.0 - 3.0 GΩ |
Not Applicable |
| Membrane Resistance (Rm) |
Not Measured |
0.2 - 2 GΩ |
0.5 - 10 MΩ |
| Data Points/6 hr Experimental Time |
2300 |
250 |
60 |
| Access Resistance (Rs) |
10 - 15 MΩ |
3 - 8 MΩ |
Not Measured |
| Rs Control |
None |
Pneumatic, Feedback Controlled |
Not Applicable |
| Rs Compensation |
None |
>=80% |
Not Applicable |
| Membrane Capacitance (Cm) Compensation |
No |
Yes |
No |
| Pipette Tips |
Fixed |
Disposable |
Disposable |
| Number of Pipettes |
12 or 48 |
1 |
8 |
| Recording During Compound Addition |
No |
Yes |
Yes |
| Time Between Compound Addition and Recording |
7 s Minimum |
Continuous Add and Read |
Continuous Add and Read |
| Compound Addition Time Constant |
Not Applicable |
tau<15 ms |
1-3 s for 90% exchange |
| Compound Wash Out |
No |
Yes |
Yes |
| Compound Wash Out Volume |
Not Applicable |
1000 mL total |
User-Configurable |
| System Control Software |
IonWorks Quattro |
PatchXpress Commander |
OpusXpress Commander |
| Data Acquisition Rate |
156 Hz – 10 kHz |
1 Hz – 31.25 kHz |
1 Hz – 31.25 kHz |
| Real Time Measurements Display |
Yes |
Yes |
Yes |
| Control of Patching Parameters |
Yes |
Yes |
Yes |
| Data Management |
No |
DataXpress 2.0 |
No |
| Data Analysis |
No |
DataXpress 2.0 |
No |
| Automated Data Analysis |
No |
Yes |
No |
