Cellular Imaging Technical Notes

Analysis

• D10077: Using Classifiers to Exclude (or Include) Unwanted Objects From Region Measurements
• D10125: Using Classifiers to Select Objects for Measuring Brightness Over Time
• D10198: Counting and Measuring Branch Points on Tree-Like Structures
• D10199: Measuring or Counting Objects Within Objects
• D10200: Using Classifiers to Exclude Unwanted Objects From Colocalization Measurements
• D20025: Using Integrated Morphometry Analysis (IMA) to Select Parts of an Image For Further Analysis
• D50004: Discovery-1 Kinetic Data: Analysis of Kinetic Data
• D50009: A Journal Set to Determine the Number of Cells Containing Spots (Gemini Bodies), Average Number of Spots per Cell, and Average Spot Size on the Discovery-1 System
• D50010: Three Journals to Determine the Number and Intensity of Cells on the Discovery-1 System
• D50025: General Tools for use with Screening Modules

Assays / Application Modules

• D50001: Running a Neurite Outgrowth Assay in the Non-database Version of the Discovery-1 System
• D50011: Determining the Number and Intensity of Positive Cells in a Transfected Population on the Discovery-1 System
• D50012: Determining the Distribution of Two (Positive) Populations over a Whole Cell Population with the Discovery-1 System
• D50019: Running the Count Nuclei Assay using the Non-database Version of the Discovery-1 System
• D50020: Running the Granularity Assay in the Non-database Version of the Discovery-1 System
• D50021: Running Angiogenesis Tube Formation Assay in the Non-database Version of the Discovery-1 System
• D50024: Analyzing a Translocation Assay on the Discovery-1 System

Software

• D10054: How is 'Standard Area' used to determine a count?
• D10179: Discovery-1 Software Region File Format
• D10243: The Discovery-1 Software STK and TIFF File Formats
• D50002: Outputting Results in 96/384 Well Format When Working in the Non Database Version of the Discovery-1 Software
• D50006: Upgrading Discovery-1 Application from Version 5.0 to 6.x
• D50014: Important Files in Discovery-1 Version 6.1
• D50015: Increasing the Z Upper Limit to Allow for Thick Samples
• D50022: Correcting for Uneven Background in an Image Using Morphology Filters
• D50023: Updating the Discovery-1 Registration Code to Enable New Modules
• D50025: General Tools for use with Screening Modules
• D50027: Imports Cellomics DIB Files into a Local, Network, or Database Location

Hardware

• D10124: Discovery-1 Software Parallel Port Pin Numbering

Variables

• D10197: Using Variables in the Discovery-1 Software to Create Concentric Regions
• D20034: How to Use Variables to Create Reusable Screening Analysis Journals for any Wavelength Combination
• D50008: Using Variables to Override Function Settings in a Journal

Journaling

• D50009: A Journal Set to Determine the Number of Cells Containing Spots (Gemini Bodies), Average Number of Spots per Cell, and Average Spot Size on the Discovery-1 System
• D50010: Three Journals to Determine the Number and Intensity of Cells on the Discovery-1 System

Display

• D10188: Display Option: Overlay Image Command
• D10211: Dialog Box Appearance

Troubleshooting

• D20002: Screen Acquisition Option Is Not Present
• D20015: Troubleshooting the Image Browser
• D50007: Troubleshooting Microsoft MSDE or Microsoft SQL Server Connections for Discovery-1 Version 6.1

Microsoft SQL Server

• D20018: Installing Microsoft SQL Server Desktop Engine Service Pack 3
• D50007: Troubleshooting Microsoft MSDE or Microsoft SQL Server Connections for Discovery-1 Version 6.1
• D50016: Using Database Scripts to Install or Update the Microsoft MSDE or Microsoft SQL Server Screening Database SR2 in Discovery-1 v6.1
• D50017: Using Database Scripts to Install or Update the Microsoft MSDE or Microsoft SQL Server Screening Database SR3 for Discovery-1, Version 6.2
• D50018: Discovery-1 Screening Database Maintenance Using Microsoft MSDE or Microsoft SQL Server SR2 or SR3

Acquisition

• D50003: Compensating for Changing Focus between Wavelengths
• D50005: Determine and Optimize Settings for Automated Image Acquisition and Auto Focus on the Discovery-1 System
• D50026: Screen Acquisition: Imaging a Thick Specimen Using a Z-Series