Headstages and Holders |
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See the Miscellaneous Products page for
model cells and adapters.
Headstages
Please note: (1) We are now only selling the 'U-type' headstages with the
threaded white Teflon collets, which connect only to HL-U holders, or U-type adapters
and model cells. (2) All adapters and holders must be ordered separately.
HS-2 and HS-2A unity-gain headstages
HS-2 and HS-2A headstages are used with the Axoclamp and the GeneClamp.
They are all unity-gain voltage recording headstages but they come in a variety of
different current-passing gains for applications as diverse as extracellular recording,
bath-potential recording, ion-sensitive recording, ionophoresis and intracellular
recording from small or large cells. For ultra-high impedance electrodes, special
circuitry inside the headstage prevents any DC current from leaking into the input
through the capacitance neutralization circuit.
Standard values of these headstages are:
| HS-2A-x100MGU | HS-2A-x1LU |
| HS-2A-x10MGU | HS-2A-x0.1LU |
| HS-2A-x1MGU | HS-2-x0.01MU |
| | HS-2-x0.0001MU |
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"M" versions have extended capacitance neutralization range.
"G" versions have grounded cases.
HS-2"A" series headstages have an extended operating voltage range (±150V).
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HS-4-x1MGU relay-switched unity-gain headstage
HS-4 headstages may be used with the Axoclamp to maximize the voltage across the electrode
during two-electrode voltage clamp. In all other modes the HS-4 acts like an HS-2 headstage.
Available only with current-passing gain x1MG. The VG-2 headstage must be used for
current measurement.
VG-2 virtual-ground headstage
VG-2 virtual ground headstage may be optionally used with the Axoclamp to measure
whole-bath current. Standard current-measurement gains are x0.1, x1 and x10.
x100 is also available. Current recording ranges for these virtual ground headstages are:
±0.1 µA (x0.1), ±1 µA (x1), ±10 µA (x10), ±100 µA (x100).
VG-2A-x100 bath clamp headstage
The VG-2A-x100 bath-clamp headstage may optionally be used with the Axoclamp or the
GeneClamp to clamp the bath potential at zero volts. This eliminates the effect of
series resistance in the bath grounding electrode and the bath solution. It can also
minimize the extent of DC voltage shifts resulting from changes in the bath solution
or temperature.
Optional CV-5 Series Headstages for the GeneClamp 500B
CV-5-100GU patch-clamp headstage (100 mV/pA)
CV-5B-100GU bilayer patch-clamp headstage (100 mV/pA)
CV-5-1GU macro-patch headstage (1 mV/pA)
CV-5-100MU voltammetry headstage (100 mV/nA)
CV-5 headstages operate with the GeneClamp 500. Each headstage has one feedback resistor
for current-to-voltage conversion. The transfer resistance of the 100G, 1G and 100M
versions are 100 GΩ, 1 GΩ and 100 MΩ, respectively. The "B" version has
an extended capacitance compensation range suitable for bilayers. The 100G version is
ideal for single-channel recording and vesicular-release amperometry. The 1G version
is suitable for macropatch applications. The 100M version is used for fast cyclic
voltammetry using carbon-fiber microelectrodes.
EX-1 five-foot (1.5 m) extension cable
EX-1 extension cable works with HS-2, HS-2A, HS-4, VG-2 and VG-2A headstages.
Mechanical Mounting Options
A 4" (102 mm) long removable insulated mounting rod is provided at no charge with most
headstages. Diameter is 5/16" (7.9 mm) unless 1/4" or 3/8" (6.3 or 9.5 mm) is specified
by purchaser. Additionally, all headstages include an insulated mounting plate. Many
manufacturers of micromanipulators provide custom mounting brackets for Axon headstages.
Holders
HL-U: to connect to all U-type headstages
(for use only with threaded headstage collets)
HL-U for 1.0-1.7 mm glass
HLB-U BNC adapter
HLR-U right-angle adapter
Four different sizes of cone washers allow the holder to accommodate pipettes
with outer diameters from 1.0 to 1.7 mm. Suction port connects to 1 mm ID tubing.
Low-noise easy-maintenance construction. Contains a 0.25 mm silver wire. Spare components
supplied are as follows: cone washers (five of each size), four 50 mm lengths of silver
wire and 70 mm of silicone tubing to make new seals. BNC adapter accepts a BNC-type
electrode holder.
Replacement Parts for HL-U Holder
| HL-CAP | | two polycarbonate caps |
| HLC-11 | | orange cone washer, pack of 5
1.1 mm ID, fits 1.0 - 1.1 mm OD glass |
| HLC-13 | | clear cone washer, pack of 5
1.3 mm ID, fits 1.1 - 1.3 mm OD glass |
| HLC-15 | | orange cone washer, pack of 5
1.5 mm ID, fits 1.3 - 1.5 mm OD glass |
| HLC-17 | | clear cone washer, pack of 5
1.7 mm ID, fits 1.5 - 1.7 mm OD glass |
| HLP-U | | 1 mm brass pin, pack of 3 |
| HLT-70 | | silicone tubing, 1 mm ID, 70 mm long |
HL-1: to connect to non-U-type CV Series headstages
(for use only with non-threaded white Teflon collets)
HL-1-12 for 1.0-1.2 mm glass
HL-1-17 for 1.5-1.7 mm glass
HLB-1 BNC adapter
HLR-1 right-angle adapter
HL-1 series holders, the HLB-1 BNC adapter, and HLR-1 right-angle adapter fit
the CV headstages previously used with the Axopatch and GeneClamp. Suction port
connects to 1 mm ID tubing. Contains a 0.25 mm silver wire. Supplied with 5 spare
'O' rings, spare wire and a spare pierced rubber seal.
HL-2: to connect to 2 mm jacks of non-U-type headstages
HL-2-12 for 1.0-1.2 mm glass
HL-2-17 for 1.5-1.7 mm glass
HLB-2 BNC adapter
HLR-2 right-angle adapter
HL-2 series holders, the HLB-2 BNC adapter, and HLR-2 right-angle adapter fit
the HS-2, HS-2A, HS-4, VG-2, VG-2A and BH-1 headstages, or any headstage with
a 2 mm jack. Suction port connects to 1 mm ID tubing. Contains a 0.25 mm
silver wire. Supplied with 5 spare 'O' rings, spare wire and a spare pierced
rubber seal.
Replacement Parts for HL-1 and HL-2 Holders
HLO-12 'O' rings fit HL-1-12 and HL-2-12 holders, pack of 10
HLO-17 'O' rings fit HL-1-17 and HL-2-17 holders, pack of 10
HLP-O general purpose 2 mm plugs, pack of 5
HLP-1 1 mm brass pins for HL-1 holders, pack of 3
HLP-2 2 mm brass pins for HL-2 holders, pack of 3
HLS-001 pierced rubber seals fit HL-1 and HL-2 holders, pack of 10
For All U-type and Non-U-type Holders
HLA-003 Ag/AgCl pellet assemblies, pack of 3
1 mm diameter AgCl pellet on the end of an Ag wire. The Ag wire is insulated from the
electrode solution by a wax-sealed Teflon tube.
HLA-005 Ag wires, pack of 5
Ag wires used in all holders. The 0.25 mm wires are 50 mm long.
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